Skip to main content

Trinity College Dublin, The University of Dublin

Trinity Menu Trinity Search



Professor Andrew Bowie
Professor of Innate Immunology, Biochemistry

Biography

Andrew Bowie obtained his PhD in Biochemistry from Trinity College Dublin in 1997. After postdoctoral training with Prof Luke O'Neill, he was appointed as lecturer in the Department of Pharmacology in UCD, before returning to TCD in 2001 to establish the first and only Immunology undergraduate degree course in Ireland, which he coordinated from 2002 - 2006. He served as Director of Research in the School of Biochemistry and Immunology from 2005 - 2009, and as Head of Immunology from 2011 - 2017. In 2008 he was elected a Fellow of TCD, and in 2014 a member of the Royal Irish Academy. He is actively involved in both undergraduate and postgraduate Immunology teaching, and has graduated 14 PhD students to date. His research discoveries and scholarly activities have significantly contributed to TCD's global reputation as a place of excellence for Immunology teaching and research and in 2017 he was awarded the Irish Society for Immunology Public Lecture Medal for his outstanding contribution to Irish Immunology. He is a global leader in the research fields of innate immunity and viral evasion, and his research occupies a unique space at the interface between virology, immunology and disease pathogenesis. He has raised research funding of >€9m (direct costs to PI) since 2001, from national and international funding agencies including Science Foundation Ireland, the EU and the US National Institutes of Health, and also from private industry. Work in his laboratory has shed light on how pattern recognition receptors (PRRs) recognise pathogens, leading to the induction of interferons and cytokines, which control infection locally as well as coordinating the adaptive immune response. He also investigates how PRRs and other innate immune sensors drive inflammation, and discovered one of the key cellular sensors of DNA in 2010, IFI16, which has subsequently been shown to be critical in understanding viral pathogenesis of diverse viruses including HIV and herpes viruses. He has recently identified novel roles for a number of innate immune proteins in regulating inflammation via cytokine modulation, and is currently exploring their mechanisms of action. His seminal discoveries have redefined our understanding of how pathogens engage with human cells, and he has regularly published research papers, opinion pieces and reviews in leading international journals including Nature Immunology, Immunity, The Journal of Experimental Medicine, Nature Reviews Immunology, PNAS, PLoS Pathogens and The EMBO Journal.

Publications and Further Research Outputs

Peer-Reviewed Publications

Carty M, Bowie AG., SARM: from immune regulator to cell executioner., Biochemical pharmacology, 2019 Journal Article, 2019 DOI

Carty, M. and Kearney, J. and Shanahan, K.A. and Hams, E. and Sugisawa, R. and Connolly, D. and Doran, C.G. and Muñoz-Wolf, N. and GÌrtler, C. and Fitzgerald, K.A. and Lavelle, E.C. and Fallon, P.G. and Bowie, A.G., Cell Survival and Cytokine Release after Inflammasome Activation Is Regulated by the Toll-IL-1R Protein SARM, Immunity, 50, (6), 2019, p1412-1424.e6 Journal Article, 2019 DOI

Bowie, A.G., Harnessing poxviral know-how for anti-cytokine therapies, Journal of Biological Chemistry, 294, (13), 2019, p5228-5229 Journal Article, 2019 DOI

Bowie, A.G., Self-RNA sentinels signal viral invasion news-and-views, Nature Immunology, 19, (1), 2018, p4-5 Journal Article, 2018 DOI

Dunphy G, Flannery SM, Almine JF, Connolly DJ, Paulus C, Jønsson KL, Jakobsen MR, Nevels MM, Bowie AG, Unterholzner L., Non-canonical Activation of the DNA Sensing Adaptor STING by ATM and IFI16 Mediates NF-"B Signaling after Nuclear DNA Damage., Molecular cell, 71, (5), 2018, p745-760.e5 Journal Article, 2018 DOI

Cooke G, Kamal I, Strengert M, Hams E, Mawhinney L, Tynan A, O' Reilly C, O' Dwyer DN, Kunkel SL, Knaus UG, Shields DC, Moller DR, Bowie AG, Fallon PG, Hogaboam CM, Armstrong ME, Donnelly SC., Toll-like receptor 3 L412F polymorphism promotes a persistent clinical phenotype in pulmonary sarcoidosis., QJM, 2018, p217-224 Journal Article, 2018 URL

Dempsey A, Keating SE, Carty M, Bowie AG., Poxviral protein E3-altered cytokine production reveals that DExD/H-box helicase 9 controls Toll-like receptor-stimulated immune responses., The Journal of biological chemistry, 293, (39), 2018, p14989-15001 Journal Article, 2018 DOI

Brady, G., Haas, D.A., Farrell, P.F., Pichlmair, A. and Bowie, A.G. , Molluscum Contagiosum Virus protein MC005 inhibits NFκB activation by targeting NEMO-regulated IKK activation, The Journal of Virology, 91, (15), 2017, p00545-17 Journal Article, 2017 DOI TARA - Full Text

Sisquella, X. and Ofir-Birin, Y. and Pimentel, M.A. and Cheng, L. and Abou Karam, P. and Sampaio, N.G. and Penington, J.S. and Connolly, D. and Giladi, T. and Scicluna, B.J. and Sharples, R.A. and Waltmann, A. and Avni, D. and Schwartz, E. and Schofield, L. and Porat, Z. and Hansen, D.S. and Papenfuss, A.T. and Eriksson, E.M. and Gerlic, M. and Hill, A.F. and Bowie, A.G. and Regev-Rudzki, N., Malaria parasite DNA-harbouring vesicles activate cytosolic immune sensors, Nature Communications, 8, (1), 2017, p1985- Journal Article, 2017 TARA - Full Text DOI

Mahony R, Gargan S, Roberts KL, Bourke N, Keating SE, Bowie AG, O'Farrelly C, Stevenson NJ, A novel anti-viral role for STAT3 in IFN-alpha signalling responses. , Cellular and Molecular Life Sciences, 74, (9), 2017, p1755 - 1764 Journal Article, 2017 DOI TARA - Full Text

Almine J., Almine J., O'Hare C., O'Hare C., Dunphy G., Dunphy G., Haga I., Naik R., Atrih A., Connolly D., Taylor J., Kelsall I., Bowie A., Beard P., Beard P., Unterholzner L., Unterholzner L., IFI16 and cGAS cooperate in the activation of STING during DNA sensing in human keratinocytes, Nature Communications, 8, 2017, p14392 Journal Article, 2017 DOI TARA - Full Text

Harte, C., Gorman, A.L., McCluskey, S., Carty, M., Bowie, A.G., Scott, C.J., Meade, K.G. and Lavelle, E.C., Alum activates the bovine NLRP3 inflammasome, Frontiers in Immunology, 8, 2017, p1494- Journal Article, 2017 DOI

Brady G, Boggan L, Bowie A, O'Neill L.A.J, Erratum: Schlafen-1 causes a cell cycle arrest by inhibiting induction of cyclin D1 (The Journal of Biological Chemistry (2005) 280 (30723-30734) DOI: 10.1074/jbc.M500435200), Journal of Biological Chemistry, 291, (48), 2016, p25278- Journal Article, 2016 DOI URL

Carroll, E.C., Jin, L., Mori, A., Muñoz-Wolf, N., Oleszycka, E., Moran, H.B.T., Mansouri, S., McEntee, C.P., Lambe, E., Agger, E.M., Andersen, P., Cunningham, C., Hertzog, P., Fitzgerald, K.A., Bowie, A.G., Lavelle, E.C., The Vaccine Adjuvant Chitosan Promotes Cellular Immunity via DNA Sensor cGAS-STING-Dependent Induction of Type I Interferons, Immunity, 44, (3), 2016, p597 - 608 Journal Article, 2016 DOI

Shirey, K.A, Lai, W., Pletneva, L.M., Patel, M., Pang, C., Kurt-Jones, E., Lipsky, M., Roger, T., Calandra, T., Tracey, K., Al Abed, Y., Bowie, A.G., Fasano, A., Dinarello, C., Gusovsky, F., Blanco, J.C.G., and Vogel S.N., Novel Strategies for Targeting Innate Immune Responses to Influenza , Mucosal Immunology , 9, 2016, p1173 - 1182 Journal Article, 2016 DOI

Brady G, Haas DA, Farrell PJ, Pichlmair A, Bowie AG, Poxvirus Protein MC132 from Molluscum Contagiosum Virus Inhibits NF-B Activation by Targeting p65 for Degradation., Journal of virology, 89, (16), 2015, p8406-15 Journal Article, 2015 TARA - Full Text DOI

Cox, D. J., Field, R. H., Williams, D. G., Baran, M., Bowie, A. G., Cunningham, C. and Dunne, A. , DNA sensors are expressed in astrocytes and microglia in vitro and are upregulated during gliosis in neurodegenerative disease, GLIA, 63, (5), 2015, p812-825 Journal Article, 2015 DOI TARA - Full Text

Wang, H., El Maadidi, S., Fischer, J., Grabski, E., Dickhöfer, S., Klimosch, S., Flannery, S.M., Filomena, A., Wolz, O-O., Schneiderhan-Marra, N., Löffler, M.W., Wiese, M., Pichulik, T., Müllhaupt, B., Semela, D., Dufour, J-F., the East-German and Swiss Hepatitis C Virus Study Groups, Bochud, P-Y., Bowie, A.G., Kalinke, U., Berg, T., Weber, A.N.R. , A frequent hypofunctional IRAK2 variant is associated with reduced spontaneous hepatitis C virus clearance, Hepatology, 62, 2015, p1375 - 1387 Journal Article, 2015 DOI

Dempsey A, Bowie AG, Innate immune recognition of DNA: A recent history., Virology, 479-480, 2015, p146-52 Journal Article, 2015 DOI TARA - Full Text

Brady, Gareth Bowie, Andrew G., Innate immune activation of NFκB and its antagonism by poxviruses, Cytokine & Growth Factor Reviews, 2014 Journal Article, 2014 TARA - Full Text DOI

Andrew Bowie, Barry Harrington, Tatyana Lysakova, Brian Keogh, 'Vaccinia virus protein A46 peptide and use thereof', EPO, US Patent No. 8,722,052, 2014 Patent, 2014 Other

Wang H, Flannery SM, Dickhöfer S, Huhn S, George J, Kubarenko AV, Lascorz J, Bevier M, Willemsen J, Pichulik T, Schafmayer C, Binder M, Manoury B, Paludan SR, Alarcon-Riquelme M, Bowie AG, Försti A, Weber AN, A coding IRAK2 protein variant compromises Toll-like receptor (TLR) signaling and is associated with colorectal cancer survival., The Journal of biological chemistry, 289, (33), 2014, p23123-31 Journal Article, 2014 TARA - Full Text DOI

Gürtler, C., Carty, M., Kearney, J., Schattgen, S.A., Ding, A., Fitzgerald, K.A. and Bowie, A.G. , SARM regulates CCL5 production in macrophages by promoting the recruitment of transcription factors and RNA polymerase II to the Ccl5 promoter, Journal of Immunology, 192, 2014, p4821 - 4832 Journal Article, 2014 TARA - Full Text

Gürtler C, Carty M, Kearney J, Schattgen SA, Ding A, Fitzgerald KA, Bowie AG, SARM regulates CCL5 production in macrophages by promoting the recruitment of transcription factors and RNA polymerase II to the Ccl5 promoter., Journal of immunology (Baltimore, Md. : 1950), 192, (10), 2014, p4821-32 Journal Article, 2014 TARA - Full Text DOI

Stack J, Doyle SL, Connolly DJ, Reinert LS, O'Keeffe KM, McLoughlin RM, Paludan SR, Bowie AG, TRAM is required for TLR2 endosomal signaling to type I IFN induction., Journal of immunology (Baltimore, Md. : 1950), 193, (12), 2014, p6090-102 Journal Article, 2014 TARA - Full Text DOI

Verstak B, Stack J, Ve T, Mangan M, Hjerrild K, Jeon J, Stahl R, Latz E, Gay N, Kobe B, Bowie AG, Mansell A, The TLR signaling adaptor TRAM interacts with TRAF6 to mediate activation of the inflammatory response by TLR4., Journal of leukocyte biology, 96, (3), 2014, p427-36 Journal Article, 2014 DOI TARA - Full Text

Stack, J., Doyle, S.L., Connolly, D.J., (...), Paludan, S.R., Bowie, A.G. , TRAM is required for TLR2 endosomal signaling to type I IFN induction, Journal of Immunology, 193, (12), 2014, p6090-6102 Journal Article, 2014 DOI TARA - Full Text

Viral Infections and the DNA Sensing Pathway: Lessons from Herpesviruses and Beyond in, editor(s)Ishii, K.J. and Tang, J.C.T. , Biological DNA Sensor: the Impact of Nucleic Acids on Diseases and Vaccinology, Elsevier, 2014, pp171 - 203, [Paludan, S.R. & Bowie, A.G. ] Book Chapter, 2014

Connolly DJ, Bowie AG, The emerging role of human PYHIN proteins in innate immunity: Implications for health and disease., Biochemical pharmacology, 2014 Journal Article, 2014 TARA - Full Text DOI

Carty M, Reinert L, Paludan SR, Bowie AG, Innate antiviral signalling in the central nervous system., Trends in immunology, 35, (2), 2014, 79-87 Journal Article, 2014 DOI

Bowie AG, Rad50 and CARD9, missing links in cytosolic DNA-stimulated inflammation., Nature immunology, 15, (6), 2014, p534-6 Journal Article, 2014

Stack J, Hurst TP, Flannery SM, Brennan K, Rupp S, Oda S, Khan AR, Bowie AG, Poxviral protein A52 stimulates p38 mitogen-activated protein kinase (MAPK) activation by causing tumor necrosis factor receptor-associated factor 6 (TRAF6) self-association leading to transforming growth factor ß-activated kinase 1 (TAK1) recruitment., The Journal of biological chemistry, 288, (47), 2013, p33642-53 Journal Article, 2013 TARA - Full Text DOI

Mansur DS, Maluquer de Motes C, Unterholzner L, Sumner RP, Ferguson BJ, Ren H, Strnadova P, Bowie AG, Smith GL, Poxvirus Targeting of E3 Ligase ß-TrCP by Molecular Mimicry: A Mechanism to Inhibit NF-"B Activation and Promote Immune Evasion and Virulence., PLoS pathogens, 9, (2), 2013, pe1003183 Journal Article, 2013 DOI TARA - Full Text

Paludan, S.R., Bowie, A.G., Horan, K.A., Fitzgerald, K.A., Recognition of herpesviruses by the innate immune system, Nature Reviews Immunology, 13, 2013, p614 Journal Article, 2013 DOI

Horan KA, Hansen K, Jakobsen MR, Holm CK, Søby S, Unterholzner L, Thompson M, West JA, Iversen MB, Rasmussen SB, Ellermann-Eriksen S, Kurt-Jones E, Landolfo S, Damania B, Melchjorsen J, Bowie AG, Fitzgerald KA, Paludan SR, Proteasomal degradation of herpes simplex virus capsids in macrophages releases DNA to the cytosol for recognition by DNA sensors., Journal of immunology (Baltimore, Md. : 1950), 190, (5), 2013, p2311-9 Journal Article, 2013 TARA - Full Text DOI

Bowie AG, Removing the TREX1 safety net: oxidized DNA overcomes immune silencing by exonuclease TREX1., Immunity, 39, (3), 2013, p423-5 Journal Article, 2013 DOI TARA - Full Text

Paludan, S., Bowie, A., Immune Sensing of DNA, Immunity , 38, (5), 2013, p870-880 Journal Article, 2013 DOI

O'Neill, L.A.J., Golenbock, D., Bowie, A.G., The history of Toll-like receptors-redefining innate immunity, Nature Reviews Immunology, 13, (6), 2013, p453-460 Journal Article, 2013 DOI TARA - Full Text

Gürtler, C., Bowie, A.G., Innate immune detection of microbial nucleic acids, Trends in Microbiology, 21, (8), 2013, p413-420 Journal Article, 2013 DOI TARA - Full Text

Bowie, A.G., The STING in the Tail for Cytosolic DNA-Dependent Activation of IRF3, Science Signalling, 5, 2012, ppe5 Journal Article, 2012 DOI

Tanveer R, Gowran A, Noonan J, Keating SE, Bowie AG, Campbell VA, The endocannabinoid, anandamide, augments Notch-1 signaling in cultured cortical neurons exposed to amyloid-ß and in the cortex of aged rats., The Journal of biological chemistry, 287, (41), 2012, p34709-21 Journal Article, 2012 DOI

Jin T, Perry A, Jiang J, Smith P, Curry JA, Unterholzner L, Jiang Z, Horvath G, Rathinam VA, Johnstone RW, Hornung V, Latz E, Bowie AG, Fitzgerald KA, Xiao TS, Structures of the HIN domain:DNA complexes reveal ligand binding and activation mechanisms of the AIM2 inflammasome and IFI16 receptor., Immunity, 36, (4), 2012, p561-571 Journal Article, 2012 DOI

Bowie, A.G., Innate sensing of bacterial cyclic dinucleotides: More than just STING, Nature Immunology, 13, (12), 2012, p1137-1139 Journal Article, 2012 DOI TARA - Full Text

Pichlmair A, Kandasamy K, Alvisi G, Mulhern O, Sacco R, Habjan M, Binder M, Stefanovic A, Eberle CA, Goncalves A, Bürckstümmer T, Müller AC, Fauster A, Holze C, Lindsten K, Goodbourn S, Kochs G, Weber F, Bartenschlager R, Bowie AG, Bennett KL, Colinge J, Superti-Furga G, Viral immune modulators perturb the human molecular network by common and unique strategies., Nature, 487, (7408), 2012, p486-490 Journal Article, 2012 DOI

Stack J, Bowie AG, Poxviral protein A46 antagonizes Toll-like receptor 4 signaling by targeting BB loop motifs in Toll-IL-1 receptor adaptor proteins to disrupt receptor:adaptor interactions., The Journal of biological chemistry, 287, (27), 2012, p22672-22682 Journal Article, 2012 DOI TARA - Full Text

Leow-Dyke S, Allen C, Denes A, Nilsson O, Maysami S, Bowie AG, Rothwell NJ, Pinteaux E, Neuronal toll-like receptor 4 signaling induces brain endothelial activation and neutrophil transmigration in vitro., Journal of neuroinflammation, 9, 2012, p230 Journal Article, 2012 DOI TARA - Full Text

Paludan, S.R., Levy, O. & Bowie, A.G., Toll2011 at Lago di Garda: studying danger sensors by the guard towers at the lake, Nature Immunology, 12, 2011, p805 - 808 Journal Article, 2011

Unterholzner, L. & Bowie, A.G., Innate DNA Sensing Moves to the Nucleus, Cell Host and Microbe, 9, 2011, p351 - 353 Journal Article, 2011 TARA - Full Text DOI

O'Neill LA, Bowie AG, The powerstroke and camshaft of the RIG-I antiviral RNA detection machine., Cell, 147, (2), 2011, p259-261 Journal Article, 2011 DOI

Leonie Unterholzner, Rebecca P. Sumner, Marcin Baran, Hongwei Ren, Daniel S. Mansur, Nollaig M. Bourke, Felix Randow, Geoffrey L. Smith, Andrew G. Bowie, Vaccinia Virus Protein C6 Is a Virulence Factor that Binds TBK-1 Adaptor Proteins and Inhibits Activation of IRF3 and IRF7, PLoS Pathogens, 7, (9, e1002247), 2011 Journal Article, 2011 TARA - Full Text

Keating, S.E., Baran, M & Bowie, A.G., Cytosolic DNA sensors regulating type I interferon induction, Trends in Immunology, 32, 2011, p574 - 581 Journal Article, 2011 TARA - Full Text DOI

Paludan, S.R., Bowie, A.G., Horan, K.A., Fitzgerald, K.A., Recognition of herpesviruses by the innate immune system, Nature Reviews Immunology, 11, 2011, p143 - 154 Journal Article, 2011 TARA - Full Text DOI

Sinead M. Flannery, Sinead E. Keating, Joanna Syzmak and Andrew G. Bowie, HUMAN INTERLEUKIN-1 RECEPTOR ASSOCIATED KINASE-2 (IRAK-2) IS ESSENTIAL FOR TOLL-LIKE RECEPTOR-MEDIATED TRANSCRIPTIONAL AND POSTTRANSCRIPTIONAL REGULATION OF TNFα, Journal of Biological Chemistry, 286, 2011, p23688 - 23697 Journal Article, 2011 TARA - Full Text DOI

Michael Carty, Andrew G. Bowie, Evaluating the role of Toll-like Receptors in diseases of the Central Nervous system, Biochemical Pharmacology, 81, (7), 2011, p825-837 Journal Article, 2011 TARA - Full Text

Carty, M., Bowie, A.G, Activation of host pattern recognition receptors by viruses, Clinical and Experimental Immunology, 161, (3), 2010, p397-406 Journal Article, 2010 TARA - Full Text DOI

Unterholzner, L., Keating, S.E., Baran, M., Horan, K.A., Jensen, S.B., Sharma, S., Sirois, C.M., (...), Bowie, A.G., IFI16 is an innate immune sensor for intracellular DNA, Nature Immunology, 11, 2010, p997-1004 Journal Article, 2010 TARA - Full Text DOI

Sinead Flannery and Andrew G. Bowie, The interleukin-1 receptor-associated kinases: critical regulators of innate immune signalling, Biochemical Pharmacology, 80, (12), 2010, p1981-1991 Journal Article, 2010 TARA - Full Text

Mulhern, O., Bowie, A.G., Unexpected roles for DEAD-box protein 3 in viral RNA sensing pathways , European Journal of Immunology, 40, (4), 2010, p933-935 Journal Article, 2010 TARA - Full Text DOI

Brennan, K., Bowie, A.G., Activation of host pattern recognition receptors by viruses, Current Opinion in Microbiology, 13, (4), 2010, p503-507 Journal Article, 2010 DOI

Lysakova-Devine, T., Keogh, B., Harrington, B., Nagpal, K., Halle, A., Golenbock, D.T., Monie, T. & Bowie, A.G. , Viral Inhibitory Peptide of TLR4, a Peptide Derived from Vaccinia Protein A46, Specifically Inhibits TLR4 by Directly Targeting MyD88 Adaptor-Like and TRIF-Related Adaptor Molecule, The Journal of Immunology, 185, 2010, p4261 - 4271 Journal Article, 2010 DOI TARA - Full Text

Carty M, Bowie AG, Recent insights into the role of Toll-like receptors in viral infection., Clinical and experimental immunology, 161, (3), 2010, p397-406 Journal Article, 2010 DOI TARA - Full Text

O'Neill, L.A.J., Bowie, A.G., Sensing and Signaling in Antiviral Innate Immunity , Current Biology , 20, (7), 2010, pR328-R333 Journal Article, 2010 DOI TARA - Full Text

Toll-like receptor 3 in, editor(s)Hansel T.T., Barnes P.J. , Progress in Respiratory Research, Karger, 2010, pp73-79 , [Carty, M., Bowie, A.] Book Chapter, 2010 DOI

Bowie, A.G, TRAF3: Uncovering the Real but Restricted Role in Human, Immunity, 33, (3), 2010, p293-295 Journal Article, 2010 TARA - Full Text DOI

Characterisation of viral proteins that inhibit TLR signal transduction in, editor(s)O'Neill, L.A.J and McCoy, C. , Toll-Like Receptors, Methods of Molecular Biology, Humana Press, 2009, [Stack, J & Bowie, A.G.] Book Chapter, 2009

Keating, SE, Bowie, AG, The role of non-degradative ubiquitination in interleukin-1 and Toll-like receptor signalling., The Journal of Biological Chemistry, 284, (13), 2009, p8211 - 8215 Journal Article, 2009 URL DOI TARA - Full Text

Role of Toll-Like Receptors in the Innate Immune Response to RNA Viruses in, editor(s)Brasier, A.R. et al. , Cellular Signaling and Innate Immune Responses to RNA Virus Infections, ASM Press, 2009, [Keating, S.E. & Bowie A.G.] Book Chapter, 2009

Uncovering novel gene function in TLR signalling using siRNA in, editor(s)O'Neill, L.A.J and McCoy, C. , Toll-Like Receptors, Methods of Molecular Biology, Humana Press, 2009, [Carty, M., Keating, S. & Bowie, A.G. ] Book Chapter, 2009

Kalverda AP, Thompson GS, Vogel A, Schröder M, Bowie AG, Khan AR & Homans SW, Poxvirus K7 protein adopts a Bcl-2 fold: Biochemical mapping of its interactions with human DEAD box RNA helicase DDX3., Journal of Molecular Biology, 385, (3), 2009, p843-853 Journal Article, 2009 DOI URL

Bowie, AG, Unterholzner, L, Viral evasion and subversion of pattern-recognition receptor signalling., Nature Reviews Immunology, 8, (12), 2008, p911 - 922 Journal Article, 2008 DOI URL

Modulation of Innate Immune Signalling Pathways by Viral Proteins in, editor(s)Fallon, P. , Pathogen-Derived Immunomodulatory Molecules, 2008, [Mulhern, O., Harrington, B. & Bowie, A.G.] Book Chapter, 2008

Bowie, A.G., TRIM-ing down Tolls, Nature Immunology, 9, (4), 2008, p348 - 350 Journal Article, 2008 URL DOI

Toll-like receptor 3 in, New Drugs and Targets for Respiratory Diseases, Prous Science, 2008, [Carty, M. & Bowie, A.G. ] Book Chapter, 2008

Unterholzner, L. & Bowie, A.G. , The interplay between viruses and innate immune signalling: recent insights and therapeutic opportunities, Biochemical Pharmacology, 75, (3), 2008, p589 - 602 Journal Article, 2008 URL DOI

Hurst, T & Bowie, A.G, Innate immune signalling pathways: lessons from vaccinia virus, Future Virology, 3, (2), 2008, p147 - 156 Journal Article, 2008 DOI URL

Schröder, M, Baran, M, Bowie, AG, Viral targeting of DEAD box protein 3 reveals its role in TBK1/IKKepsilon-mediated IRF activation., The EMBO journal, 27, (15), 2008, p2147 - 2157 Journal Article, 2008 DOI URL

Keating, S.E., Maloney, G.M., Moran, E.M. & Bowie, A.G, IRAK-2 participates in multiple toll-like receptor signalling pathways to NFkappaB via activation of TRAF6 ubiquitination, The Journal of Biological Chemistry, 282, (46), 2007, p33435 - 33443 Journal Article, 2007 DOI URL

Andrew Bowie, Luke O'Neil, Julianne Stack, Geoff Smith, Ismar Haga, 'Orthopoxvirus vectors, genes and products thereof (A46R protein)', EPO, WO2004/031225, 2007 Patent, 2007 Other

Bowie, A.G., Recent advances in understanding the role of Toll-like receptors in anti-viral immunity, Clinical and Experimental Immunology, 147, (2), 2007, p217 - 226 Journal Article, 2007 URL URL DOI

Innate Immune Signalling Pathways and Viral Recognition: New Perspectives in, editor(s)Gavin McAlpine , Focus on Signal Transduction Research, Nova Science Publishers, 2007, pp173 - 188, [Ismar R Haga & Andrew G Bowie] Book Chapter, 2007

O'Neill LA and Bowie AG, The family of five: TIR domain containing adapters in TLR signalling, Nat Rev Immunol, 7, 2007, p353-364 Journal Article, 2007 URL DOI

Bowie, A.G. & Fitzgerald, K.F. , RIG-I; Tri-ing to discriminate between self and non-self RNA, Trends in Immunology, 28, (4), 2007, p147 - 150 Journal Article, 2007 URL DOI

Marshall-Clarke,S., Downes, J.E., Haga, I.R., Bowie, A.G., Borrow, P., Pennock, J.L. Grencis, R.K. & Rothwell, P, Polyinosic acid is a ligand for Toll-like receptor 3, Journal of Biological Chemistry, 282, (34), 2007, p24759 - 24766 Journal Article, 2007 DOI URL

Andrew Bowie, Martina Schroeder, Geoff Smith, Stuart Lucas, 'Compositions and methods for modulating an immune response (K7 protein)', EPO, WO 2008/023077 , 2006 Patent, 2006 Other

Andrew Bowie, Michael Carry, Martina Schroeder, Paul Moynagh, 'Compositions and methods for modulating an immune response (SARM)', IPO, WO 2007/096854, 2006 Patent, 2006 Other

Boughan, P.K., Argent, R.H., Body-Malapel, M., Park, J-H., Ewings, K.E., Bowie, A.G., Ong, S.J., Cook, S.J., Sorensen, O.E., Manzo, B.A., Klein, N.J. Nuñez, G., Atherton, J.C. & Bajaj-Elliott, M., Nucleotide-binding oligomerisation domain-1 (NOD-1) and epidermal growth factor receptor (EGFR): critical regulators of Ò-defensins during Helicobacter Pylori infection, The Journal of Biological Chemistry, 281, (17), 2006, p11637 - 11648 Journal Article, 2006 URL DOI

Carty, M., Goodbody, R., Schröder, M., Stack, J., Moynagh, P.N. & Bowie, A.G. , The human adaptor SARM negatively regulates adaptor protein TRIF-dependent Toll-like receptor signalling, Nature Immunology, 7, (10), 2006, p1074 - 1081 Journal Article, 2006 DOI URL

Brady, G, Boggan, L, Bowie, AG & O'Neill, LAJ, Schlafen-1 causes cell cycle arrest by inhibiting induction of cyclin D1, The Journal of Biological Chemistry, 280, 2005, p30723 - 30734 Journal Article, 2005 URL

Schroeder, M & Bowie AG, TLR3 in antiviral immunity: key player or bystander?, Trends in Immunology, 26, (9), 2005, p462 - 468 Journal Article, 2005 URL DOI

Stack, J. Haga, I.R., Schroeder, M., Bartlett, N.W., Maloney, G., Reading, P.C., Fitzgerald, K.A., Smith, G.L. & Bowie, A.G, Vaccinia virus protein A46R targets multiple Toll-like-interleukin-1 receptor adaptors and contributes to virulence, The Journal of Experimental Medicine, 201, (6), 2005, p1007 - 1018 Journal Article, 2005 URL DOI TARA - Full Text

Carroll, T.P., Greene, C.M., Taggart, C.C., Bowie, A.G., O'Neill, S.J. & McElvaney, N.G., Viral inhibition of IL-1 and neutrophil elastase-induced inflammatory responses in bronchial epithelial cells, The Journal of Immunology, 175, (11), 2005, p7594 - 7601 Journal Article, 2005 URL

O'Toole D, Abde;-Latif AM, Long A, Windle HJ, Murphy AM, Bowie A, O'Neill LA, Weir DG, Kelleher D, Low pH and Helicobacter pylori increase nuclear factor kappa B binding in gastric epithelial cells: a common pathway for epithelial cell injury?, Journal of Cellular Biochemistry, 96, (3), 2005, p589 - 588 Journal Article, 2005 URL DOI

Bowie, A.G. & Haga, I.R. , The role of Toll like receptors in the host response to viruses. , Molecular Immunology, 42, 2005, p859 - 867 Journal Article, 2005 URL

Toll-like receptors as key sensors of viral infection in, editor(s)L.A.J. O'Neill and E. Brint , Toll-like Receptors in Inflammation, 2005, pp143 - 171, [Keating, S.E. and Bowie, A.G.] Book Chapter, 2005

Haga, I.H. and Bowie, A.G., Evasion of Innate Immunity by Vaccinia Virus, Parasitology, 130, 2005, pS11 - S25 Journal Article, 2005 DOI URL

Melchjorsen, J, Jensen, S.B., Malmgaard,L., Rasmussen, S.B., Weber, F., Bowie, A.G., Matikainen, S. & Paludan, S.R. , Activation of Innate Defense against a Paramyxovirus Is Mediated by RIG-I and TLR7 and TLR8 in a Cell-Type-Specific Manner, The Journal of Virology, 79, (20), 2005, p12944 - 12951 Journal Article, 2005 DOI URL

Maloney, G, Schroeder, M & Bowie, AG, Vaccinia virus protein A52R activates p38 mitogen-activated protein kinase and potentiates lipopolysaccharide-induced interleukin-10, The Journal of Biological Chemistry, 280, (280), 2005, p30838 - 30844 Journal Article, 2005 DOI

Bowie, A.G., Zhan, J. & Marshall, W.L., Viral Appropriation of Apoptotic and NF-kappaB Signalling Pathways, Journal of Cell Biology, 91, (6), 2004, p1099 - 1108 Journal Article, 2004 DOI URL

DiPerna, G., Stack, J., Bowie, A.G., Boyd, A., Kotwal, G., Zhang, Z., Arvikar, S., Latz, E., Fitzgerald, K.A. & Marshall, W.L. , Poxvirus protein N1L targets the I-kappaB Kinase complex, inhibits signaling to NF-kappaB by the Tumor Necrosis Factor superfamily of receptors, and inhibits NF-kappaB and IRF3 signaling by Toll-like receptors. , The Journal of Biological Chemistry, 279, (35), 2004, p36570 - 36578 Journal Article, 2004 URL DOI

Malmgaard, L., Melchjorsen, J., Bowie, A.G., Mogensen, S.C. & Paludan, S.R. , Viral activation of macrophages through TLR-dependent and -independent pathways., The Journal of Immunology, 173, (11), 2004, p6890 - 6898 Journal Article, 2004 URL

Harte, MT, Haga, I, Maloney, G, Gray, P, Reading, PC, Bartlett, N, Smith, GL, Bowie, AG and O'Neill, LAJ , The poxvirus protein A52R targets Toll-like receptor signaling complexes to suppress host defence, Journal of Experimental Medicine, 197, (3), 2003, p343 - 351 Journal Article, 2003 DOI TARA - Full Text

Andrew Bowie, Luke O'Neill, Mary Harte, Geoff Smith, Ismar Haga, Geraldine Maloney , 'Orthopoxvirus vectors, genes and products thereof (A52R protein). ', EPO, WO 2004/022762, 2003 Patent, 2003 Other

L. A. O'Neill, K. A. Fitzgerald and A. G. Bowie, The Toll-IL-1 receptor adaptor family grows to five members, Trends in immunology, 24, (6), 2003, p286-90 Journal Article, 2003 URL URL DOI

Modulation of Toll-like receptor signalling by viruses in, editor(s)C.D O'Connor and D.G.E Smith , SGM symposium 62: Microbial subversion of host cells, Cambridge, Cambridge University Press, 2003, pp109 - 128, [Bowie, A. ] Book Chapter, 2003

K. A. Fitzgerald, E. M. Palsson-McDermott, A. G. Bowie, C. A. Jefferies, A. S. Mansell, G. Brady, E. Brint, A. Dunne, P. Gray, M. T. Harte, D. McMurray, D. E. Smith, J. E. Sims, T. A. Bird and L. A. O'Neill, Mal (MyD88-adapter-like) is required for Toll-like receptor-4 signal transduction, Nature, 413, (6851), 2001, p78-83 Journal Article, 2001 URL URL DOI

C. Jefferies, A. Bowie, G. Brady, E. L. Cooke, X. Li and L. A. O'Neill, Transactivation by the p65 subunit of NF-kappaB in response to interleukin-1 (IL-1) involves MyD88, IL-1 receptor-associated kinase 1, TRAF-6, and Rac1, Molecular and Cellular Biology, 21, (14), 2001, p4544-4552 Journal Article, 2001 DOI

O'Neill, LAJ and Bowie, A (eds.), Interleukin protocols, New Jersey, The Humana Press Inc, 2001 Book, 2001

A. Bowie, E. Kiss-Toth, J. A. Symons, G. L. Smith, S. K. Dower and L. A. O'Neill, A46R and A52R from vaccinia virus are antagonists of host IL-1 and toll-like receptor signaling, Proceedings of the National Academy of Sciences of the United States of America, 97, (18), 2000, p10162-10167 Journal Article, 2000 DOI TARA - Full Text URL

K. A. Fitzgerald, A. G. Bowie, B. S. Skeffington and L. A. O'Neill, Ras, protein kinase C zeta, and I kappa B kinases 1 and 2 are downstream effectors of CD44 during the activation of NF-kappa B by hyaluronic acid fragments in T-24 carcinoma cells, Journal of Immunology , 164, (4), 2000, p2053-2063 Journal Article, 2000 URL

A. G. Bowie and L. A. O'Neill, Vitamin C inhibits NF-kappa B activation by TNF via the activation of p38 mitogen-activated protein kinase, Journal of Immunology, 165, (12), 2000, p7180-7188 Journal Article, 2000 URL TARA - Full Text

A. Bowie and L. A. O'Neill, The interleukin-1 receptor/Toll-like receptor superfamily: signal generators for pro-inflammatory interleukins and microbial products, Journal of Leukocyte Biology, 67, (4), 2000, p508-514 Journal Article, 2000 URL TARA - Full Text

A. Bowie and L. A. O'Neill, Oxidative stress and nuclear factor-kappaB activation: a reassessment of the evidence in the light of recent discoveries, Biochemical pharmacology, 59, (1), 2000, p13-23 Journal Article, 2000 DOI URL URL

McGowan AJ, Bowie AG, O'Neill LA, Cotter TG., The production of a reactive oxygen intermediate during the induction of apoptosis by cytotoxic insult., Experimental Cell Research, 238, (1), 1998, p248 - 256 Journal Article, 1998 DOI URL

A. G. Bowie, P. N. Moynagh and L. A. O'Neill, Lipid peroxidation is involved in the activation of NF-kappaB by tumor necrosis factor but not interleukin-1 in the human endothelial cell line ECV304. Lack of involvement of H2O2 in NF-kappaB activation by either cytokine in both primary and transformed endothelial cells, The Journal of biological chemistry, 272, (41), 1997, p25941-25950 Journal Article, 1997 URL

Bowie, A., Owens, D., Collins, P., Johnson, A. and Tomkin, G.H. , Glycosylated low density lipoprotein is more sensitive to oxidation: implications for the diabetic patient? , Atherosclerosis , 102, (1), 1993, p63 - 67 Journal Article, 1993 DOI URL

Non-Peer-Reviewed Publications

Bowie, A.G., Insights from vaccinia virus into Toll-like receptor signalling proteins and their regulation by ubiquitin: role of IRAK-2, Biochemistry Society Transactions, 36, (3), 2008, pp449-452 Conference Paper, 2008 URL

M. Schröder and A. G. Bowie, An arms race: Innate anti-viral responses and counter-acting viral strategies., Biochemistry Society Transactions, Biochemistry Society Transactions, 35, (6), 2007, pp1512-1514 Conference Paper, 2007 URL

Bowie, A. and O'Neill, L.A.J., Vitamin C inhibits NF kappa B activation in endothelial cells., Biochemistry Society Transactions, 25, (1), 1997, pp131S. Conference Paper, 1997

Bowie, A. and O'Neill, L.A.J., Studies into the mechanism of NF kappa B activation by IL1, TNF and H2O2 in primary and transformed endothelial cells., Biochemistry Society Transactions, 25, (1), 1997, pp125S Conference Paper, 1997

A. Bowie, P. N. Moynagh and L. A. O'Neill, Mechanism of NF kappa B activation by interleukin-1 and tumour necrosis factor in endothelial cells, 24, (1), 1996, pp2S Conference Paper, 1996 URL URL

A. Bowie, P. N. Moynagh and L. A. O'Neill, The human endothelial cell line ECY304 as a model of endothelial cell activation by interleukin-1, 23, (1), 1995, pp109S Conference Paper, 1995 URL URL

Brennan, P., Bowie, A. and O'Neill, L.A.J., The Effect of Thiol Modifiers on the Activation of NF kappa B by Interleukin-1., 21, (4), 1993, pp390S Conference Paper, 1993

Research Expertise

Description

My research focuses on innate immune sensing and signalling mechanisms, and their modulation by viruses. Specifically we are looking at how viruses interact with, and seek to subvert innate immune signalling pathways, such Toll-like receptors (TLRs) and RIG-I-like receptors (RLRs). TLRs and RLRs represent host immune detection systems, in that they have been shown to be involved in recognising pathogens and activating the innate immune response. Through both bioinformatics and functional screens we have identified many poxviral proteins which can antagonise innate immune signalling, and current on-going investigations are seeking to understand the mechanism of action of these proteins and to identify and characterise host target proteins. Further, peptides derived from inhibitory viral proteins are being tested as a route to developing novel anti-inflammatory drugs. Work in my laboratory has shed light on how pattern recognition receptors (PRRs) recognise pathogens, leading to the induction of interferons and cytokines, which control infection locally as well as coordinating the adaptive immune response. We also investigate how PRRs and other innate immune sensors drive inflammation through the recognition of nucleic acid such as mislocalised self-DNA. I have recently identified novel roles for a number of innate immune proteins in regulating inflammation via cytokine modulation, and am currently exploring their mechanisms of action

Keywords

Biochemistry; Cellular Biology; CYTOKINES; DNA SENSING; DNA transcription and translation; Eukaryotic gene regulation; Host, Pathogen interactions; Immune system; Immunology, Immunotherapy; Infectious diseases; Inflammation; Innate Immunity; INTERLEUKIN-1 SIGNAL TRANSDUCTION; Intra and intercellular signalling; Molecular Biology; NF-kappaB; PATTERN RECOGNITION RECEPTORS; Protein sequence, function and expression; Regulatory methods of gene expression; Signal Transduction; Toll-Like Receptors; Viral Evasion; Virology and viral pathogenesis; VIRUSES

Recognition

Awards and Honours

Highly cited researcher (Clarivate, top 1% by citations) 2018

Irish Society for Immunology Public Lecture Award Medal 2017

Elected to the Royal Irish Academy 2014

Elected Fellow of TCD 2008

Sheldon Wolff Prize in Cytokine Research 1999

Placed first in class (B.A. (Mod)). 1993

Memberships

Royal Irish Academy 15 March 2014

The International Cytokine and Interferon Society

American Society for Biochemistry and Molecular Biology

American Society for Microbiology