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Dr. Michael Monaghan
Associate Professor, Mechanical, Manuf & Biomedical Eng

Biography

Dr. Michael Monaghan is an Associate Professor in Biomedical Engineering at Trinity College Dublin, the University of Dublin. His group's primary research areas are in biomaterials (in particular electroconductive biomaterials), advanced processing and rational design of biomaterials, cardiac tissue regeneration, and real-time imaging of extracellular matrix components and metabolism in differentiation and disease. He leads a number of interdiciplinary projects between other academic groups and industry ranging from immunometabolism, evaluation of fibrosis, biomaterial synthesis and induced pluripotent stem cell derivitisation of cardiac organoids.

Dr. Monaghan performed his Postdoctoral research in Germany as a recipient of a prestigious Marie Curie Individual Fellowship. His research was coordinated between the Department of Cell and Tissue Engineering in the Fraunhofer Institute for Interfacial Engineering and Biotechnology and the Department of Women's Health in University Clinic Tubingen. During this period he has published a number of key papers in the field of human valvulogenesis, embryonic stem cell research, cardiomyocyte differentiation, biomaterials and non-invasive optical characterisation (Raman microspectroscopy, fluorescent lifetime imaging (FLIM), multiphoton and second harmonic generation (SHG) imaging). Dr. Monaghan received both his B.Eng (Biomedical) and Ph.D. (Biomedical Engineering) from the National University of Ireland Galway (NUIG). During his Ph.D. Dr. Monaghan received a number of research awards such as travel awards from the European Molecular Biology Organisation (EMBO) and the German Academic Exchange Service (DAAD), and the 2015 Julia Polak European Doctorate Award in recognition of the achievements made during his Ph.D.

Dr. Monaghan is actively involved in the tissue engineering and regenerative medicine international society (TERMIS); previously as Chair of the EU Student and Young Investigator Section, and recently promoted full EU Council Member. He is council member of Matrix Biology Ireland and acts as treasurer of this society. More details of his groups resources and research can be found on the groups website.

Lab Website: www.monaghanlab.com

Publications and Further Research Outputs

Peer-Reviewed Publications

Monaghan MG, Non-invasive classification of macrophage polarisation by 2P-FLIM and machine learning, Meeting of the European Chapter of the Tissue Engineering and Regenerative Medicine International Society, Manchester, UK, March 2023, 2023 Conference Paper, 2023

Monaghan MG, Crosslinked extracellular matrix biomaterials functionalized with 2D-nanomaterial MXenes for cardiac tissue engineering drive iPSC-derived cardiomyocyte maturation, Meeting of the European Chapter of the Tissue Engineering and Regenerative Medicine International Society, Manchester. UK, March 2023, 2023 Conference Paper, 2023

Meenakshi Suku, Lesley Forrester, Manus Biggs, Michael Monaghan, Functional cardiac organoids modeling the inflammatory environment of myocardial infarction , Meeting of the European Chapter of the Tissue Engineering and Regenerative Medicine International Society, Manchester, UK, March 2023, 2023 Conference Paper, 2023

Nuno G.B. Neto, Sinead O"Rourke, Mimi Zhang, Hannah Fitzgerald, Aisling Dunne, Michael Monaghan, Non-Invasive classification of macrophage polarisation by 2P-FLIM and machine learning, Microscopy Society of Ireland 2022 Meeting, Galway, Ireland, 2022 Conference Paper, 2022

Solazzo, Matteo, Hartzell, Linette, O"Farrell, Ciara, Monaghan, Michael G., Beyond Chemistry: Tailoring Stiffness and Microarchitecture to Engineer Highly Sensitive Biphasic Elastomeric Piezoresistive Sensors, ACS Applied Materials & Interfaces, 14, (17), 2022, p19265-19277 Journal Article, 2022 DOI

Leikeim, Anna, Wußmann, Maximiliane, Schmidt, Freia F., Neto, Nuno G. B., Benz, Franziska, Tiltmann, Kendra, Junger, Corinna, Monaghan, Michael G., Schilling, Bastian, Groeber-Becker, Florian K., A preclinical model of cutaneous melanoma based on reconstructed human epidermis, Scientific Reports, 12, (1), 2022 Journal Article, 2022 DOI

Leahy, L.M. Woods, I., Gutierrez-Gonzalez, J, Monaghan, M. G, Dervan, A., O"Brien, F.J., DEVELOPMENT OF AN ELECTROCONDUCTIVE, 3D-PRINTED SCAFFOLD DESIGNED TO PROMOTE AXONAL REGROWTH AFTER SPINAL CORD INJURY, European Society for Biomaterials Annual Meeting 2022, Bordeaux, France, September 2022, 2022 Conference Paper, 2022

Nuno Neto, Sinead O'Rourke, Mimi Zhang, Hannah Fitzgerald, Aisling Dunne and Michael Monaghan, Non-Invasive classification of macrophage polarisation by 2P-FLIM and machine learning, eLife, 11, 2022, pe77373 Journal Article, 2022

Solazzo, Matteo, Monaghan, Michael G., Hydrophilic, conductive and flexible piezoresistive PEDOT:PSS 3D sensors with tunable microarchitecture and crosslinked using a PEGylated crosslinker, Synthetic Metals, 290, 2022, p117157 Journal Article, 2022 DOI

Lianne Shanley, Olwyn Mahon, Sinead O'Rourke, Nuno Neto, Michael Monaghan, Daniel Kelly, Aisling Dunne, PARTICLE SIZE ALTERS MACROPHAGE METABOLISM: IMPLICATIONS FOR BONE TISSUE ENGINEERING, Irish Society for Immunology 2022 Annual Meeting, Maynooth, Co. Kildare, 1-2nd September 2022, 2022 Conference Paper, 2022

Leahy, L.M., Woods, I., Gutierrez-Gonzalez, J., Monaghan, M. G, Dervan, A. O"Brien, F.J, DEVELOPMENT OF AN ELECTROCONDUCTIVE, 3D-PRINTED SCAFFOLD DESIGNED TO PROMOTE AXONAL REGROWTH AFTER SPINAL CORD INJURY, Meeting of the European Chapter of the Tissue Engineering and Regenerative Medicine International Society, Krakow, Poland, July 2022, 2022 Conference Paper, 2022

Mary Rooney, Nuno Neto, Michael Monaghan, Emmeline Hill, Richard K. Porter, Conditionally immortalised equine skeletal muscle cell lines for in vitro analysis, Biochemistry and Biophysics Reports, 2022 Journal Article, 2022

Monaghan MG, Non-Invasive classification of macrophage polarisation by 2P-FLIM and machine learning, Meeting of the European Chapter of the Tissue Engineering and Regenerative Medicine International Society, Krakow, Poland, July 2022, 2022 Conference Paper, 2022

Fitzgerald, H.K.; O'Rourke, S.A.; Desmond, E.; Neto, N.G.B.; Monaghan, M.G.; Tosetto, M.; Doherty, J.; Ryan, E.J.; Doherty, G.A.; Nolan, D.P.; Fletcher, J.M.; Dunne, A, The Trypanosoma brucei-Derived Ketoacids, Indole Pyruvate and Hydroxyphenylpyruvate, Induce HO-1 Expression and Suppress Inflammatory Responses in Human Dendritic Cells, Antioxidants, 11, 2022, p164- Journal Article, 2022 DOI

Floudas A, Gorman A, Neto N, Monaghan MG, Elliott Z, Fearon U, Marzaioli V., Inside the Joint of Inflammatory Arthritis Patients: Handling and Processing of Synovial Tissue Biopsies for High Throughput Analysis., Frontiers in medicine, 9, 2022, p830998 Journal Article, 2022 DOI

Michael Monaghan, Optimization, Prediction and Smart Design for Anisotropically and Mechanically Matching Biomaterial Scaffolds Using Melt-Electrowriting, Gordon Research Conference: Bioinspired Materials, Les Diablerets, Switzerland, June 2022, 2022 Conference Paper, 2022

Michael Monaghan, Machine Learning Optimization, Prediction and Smart Design for Biomaterial Scaffolds Using Melt-Electrowriting, Biofabrication 2022- International Society for Biofabrication Meeting, Montecatini Terme, Italy, September 2022, 2022 Invited Talk, 2022

O"Rourke S., Solazzo M., Dunne A., Monaghan M.G, External delivery of electrical stimulation promotes M2 macrophage polarisation: Implications in regenerative tissue engineering, Trinity Centre for Biomedical Engineering Annual Research Day. , TCD, Dublin, 6th of December 2022, 2022 Conference Paper, 2022

Sinead A. O'Rourke, Nuno G.B. Neto, Eimear Devilly, Lianne C Shanley, Hannah K Fitzgerald, Michael G Monaghan, Aisling Dunne, INVESTIGATING METABOLIC REPROGRAMMING OF MACROPHAGES AS A NOVEL THERAPEUTIC STRATEGY FOR ATHEROSCLEROSIS, Irish Society for Immunology 2022 Annual Meeting, Maynooth, Co. Kildare, 1-2nd September 2022, 2022 Meeting Abstract, 2022

Solazzo M, Monaghan MG, ELECTROCONDUCTIVE SCAFFOLDS FOR IN VITRO CARDIAC MODELS, Meeting of the European Chapter of the Tissue Engineering and Regenerative Medicine International Society, Krakow, Poland, July 2022, 2022 Conference Paper, 2022

Neto N., O'Rourke S., Zhang M., Dunne A., Monaghan M, Single-cell metabolic profiling of macrophage polarization by fluorescence lifetime imaging microscopy (FLIM), 6th European Congress for Immunology Meeting, Online, 1-4th September, 2021 Oral Presentation, 2021

Coulter, Fergal B., Levey, Ruth E., Robinson, Scott T., Dolan, Eimear B., Deotti, Stefano, Monaghan, Michael, Dockery, Peter, Coulter, Brian S., Burke, Liam P., Lowery, Aoife J., Beatty, Rachel, Paetzold, Ryan, Prendergast, James J., Bellavia, Gabriella, Straino, Stefania, Cianfarani, Francesca, Salamone, Monica, Bruno, Carmelo M., Moerman, Kevin M., Ghersi, Giulio, Duffy, Garry P., O'Cearbhaill, Eoin D., Soft Tissue Implants: Additive Manufacturing of Multi"Scale Porous Soft Tissue Implants That Encourage Vascularization and Tissue Ingrowth (Adv. Healthcare Mater. 14/2021), Advanced Healthcare Materials, 10, (14), 2021, p2170063 Journal Article, 2021 DOI

Saha, Uttariyo, Nairn, Rory, Keenan, Orla, Monaghan, Michael G., A Deeper Insight into the Influence of the Electric Field Strength When Melt"Electrowriting on Non"Planar Surfaces, Macromolecular Materials and Engineering, 2021, p2100496 Journal Article, 2021 DOI

Fitzgerald H., Williams D. G., Campbell N. K., Barry P, O'Rourke S. A. Neto N., Monaghan M. G., Nolan D. P., Dunne A, Aromatic ketoacids represent novel therapies for autoimmune diseases through activation of the Nrf2/HO-1 pathway and suppression of pro-inflammatory responses in primary human immune cells, British Society for Immunology 2021 Congress, Edinburgh, 30th November, 2021 Meeting Abstract, 2021

Perottoni S.*, Neto N., di Nitto C., Dmitriev R., Raimoundi M.T., Monaghan M, ., Intracellular label-free detection of mesenchymal stem cell metabolism within a perivascular niche-on-a-chip, European Society for Biomechanics, Milan, Italy (and online), 11-14th July, 2021 Oral Presentation, 2021

Floudas A, Neto N, Orr C, Canavan M, Gallagher P, Hurson C, Monaghan MG, Nagpar S, Mullan RH, Veale DJ, Fearon U., Loss of balance between protective and pro-inflammatory synovial tissue T-cell polyfunctionality predates clinical onset of rheumatoid arthritis., Annals of the rheumatic diseases, 2021 Journal Article, 2021 DOI

Meenakshi Suku, Lesley Forrester, Manus Biggs, Michael G. Monaghan, Resident macrophages and their potential in cardiac tissue engineering, Tissue Engineering Part B Reviews, 2021 Journal Article, 2021 TARA - Full Text

Shanley L., Mahon O. R., O'Rourke S. A., Neto N., Monaghan M. G., Kelly D., Dunne A, Particle size alters macrophage metabolism: Implications for bone tissue engineering, British Society for Immunology 2021 Congress, Edinburgh, 30th November, 2021 Meeting Abstract, 2021

Perottoni S, Neto NGB, Di Nitto C, Dmitriev RI, Raimondi MT, Monaghan MG., Intracellular label-free detection of mesenchymal stem cell metabolism within a perivascular niche-on-a-chip., Lab on a chip, 21, (7), 2021, p1395-1408 Journal Article, 2021 DOI

Neto N., Perottoni S., di Nitto C., Dmitriev R., Raimoundi M.T., Monaghan M, ., Intracellular label-free detection of mesenchymal stem cell metabolism within a perivascular niche-on-a-chip, Termis Workshop: Cell-Matrix Interaction and Mechanobiology in Regenerative Medicine, Online, 11-12th June, 2021 Oral Presentation, 2021

Solazzo M, Monaghan MG., Structural crystallisation of crosslinked 3D PEDOT:PSS anisotropic porous biomaterials to generate highly conductive platforms for tissue engineering applications., Biomaterials science, 9, (12), 2021, p4317-4328 Journal Article, 2021 DOI

Old Drugs, New Tricks - Redefining Therapeutic Strategies For Tissue Regeneration., Advanced Drug Delivery Reviews- Elsevier, 2021, - Critical Edition (Chapter), 2021 DOI

ORourke S., Neto N., Devilly E., Shanley L., Dunne A., Monaghan M. G, Targeting metabolism in cardiovascular disease: investigating metabolic reprogramming of macrophages in atherosclerosis as a novel therapeutic strategy, British Society for Immunology 2021 Congress, Edinburgh, 30th November, 2021 Meeting Abstract, 2021

Perottoni S.*, Neto N., di Nitto C., Dmitriev R., Raimoundi M.T., Monaghan M, Intracellular label-free detection of mesenchymal stem cell metabolism within a perivascular niche-on-a-chip, European Society for Biomechanics, Milan, Italy (and online), 11-14th July, 2021 Oral Presentation, 2021

Olvera D., Sohrabi Molina M., Hendy G., Monaghan M.G., Electroconductive Melt Electrowritten Patches Matching the Mechanical Anisotropy of Human Myocardium, Advanced Functional Materials, 2020 Journal Article, 2020 DOI TARA - Full Text

Ukkelman IA, Neto N, Papkovsky DB, Monaghan MG, Dmitriev RI., A deeper understanding of intestinal organoid metabolism revealed by combining fluorescence lifetime imaging microscopy (FLIM) and extracellular flux analyses., Redox biology, 30, 2020, p101420 Journal Article, 2020 TARA - Full Text DOI

Ozaki, Ema, Gibbons, Luke, Neto, Nuno GB, Kenna, Paul, Carty, Michael, Humphries, Marian, Humphries, Pete, Campbell, Matthew, Monaghan, Michael, Bowie, Andrew and Doyle, Sarah L, SARM1 deficiency promotes rod and cone photoreceptor cell survival in a model of retinal degeneration, Life Science Alliance, 3, (5), 2020 Journal Article, 2020 TARA - Full Text DOI

Seeing Is Believing: Noninvasive Microscopic Imaging Modalities for Tissue Engineering and Regenerative Medicine in, editor(s)Heinz Redl, Jeff Gimble, Darja Marolt Presen, Richard Oreffo and Susanne Wolbank , Tissue Engineering and Regeneration. Cell Engineering and Regeneration, Switzerland, Springer, 2020, ppAccepted- - In Press, [Neto N, Dmitriev R, Monaghan MG] Book Chapter, 2020

Floudas A, Neto N, Marzaioli V, Murray K, Moran B, Monaghan MG, Low C, Mullan RH, Rao N, Krishna V, Nagpal S, Veale DJ, Fearon U., Pathogenic, glycolytic PD-1+ B cells accumulate in the hypoxic RA joint., JCI insight, 5, (21), 2020 Journal Article, 2020 DOI

Extrusion-based additive manufacturing techniques for biomedical applications in, editor(s)Dermot Brabazon , Encyclopedia of Materials: Composites, Philadelphia, Elsevier, 2020, ppIn Press - [Tadayyon G, Kelly DJ, Monaghan MG] Book Chapter, 2020

Floudas A., Neto N., Vivianna M., Murray K., Moran B., Monaghan M., Candice L., Mullan R.H., Krishna V., Nagpal S., Veale D.J., Fearon U, Pathogenic, Glycolitic PD-1+ cells accumulate in the Hypoxic RA joint, 8th Annual Meeting of the International Cytokine and Interferon Society, Seattle, USA, 1-4th November 2020, 2020 Oral Presentation, 2020

Olvera D, Monaghan MG, Electroactive biomaterial-based biosensors for detection and drug delivery , Advanced Drug Delivery Reviews, 2020 Journal Article, 2020

O'Rourke SA, Dunne A, Monaghan MG., The Role of Macrophages in the Infarcted Myocardium: Orchestrators of ECM Remodeling., Frontiers in cardiovascular medicine, 6, 2019, p101 Journal Article, 2019 DOI TARA - Full Text

Solazzo, M.*, Monaghan, M. G, Minimally invasive 3D electroconductive biomaterial platforms for cardiac tissue engineering applications, 25th Bioengineering in Ireland 2019, Limerick, Ireland, 18-19th January, 2019 Oral Presentation, 2019

Solazzo M, O'Brien FJ, Nicolosi V, Monaghan MG., The rationale and emergence of electroconductive biomaterial scaffolds in cardiac tissue engineering., APL Bioeng, 3, (4), 2019, p041501 Journal Article, 2019 DOI TARA - Full Text

O'Rourke S., Dunne A., Monaghan, M.G, The Influence of Electroconductive Materials on Macrophage Phenotype, 25th Bioengineering in Ireland 2019, Limerick, Ireland, 2019 Oral Presentation, 2019

Dolan, E.B. and Hofmann, B. and de Vaal, M.H. and Bellavia, G. and Straino, S. and Kovarova, L. and Pravda, M. and Velebny, V. and Daro, D. and Braun, N. and Monahan, D.S. and Levey, R.E. and O'Neill, H. and Hinderer, S. and Greensmith, R. and Monaghan, M.G. and Schenke-Layland, K. and Dockery, P. and Murphy, B.P. and Kelly, H.M. and Wildhirt, S. and Duffy, G.P., A bioresorbable biomaterial carrier and passive stabilization device to improve heart function post-myocardial infarction, Materials Science and Engineering C, 103, (109751), 2019 Journal Article, 2019 DOI

Dolan E. B., Varela C. E., Mendez K., Whyte W., Levey R. E., Robinson S. T., Maye E., O†Dwyer J., Beatty R., Rothman A., Fan Y., Hochstein J., Rothenbucher S. E., Wylie R., Starr J. R., Monaghan M., Dockery P., Duffy G. P., Roche E. T., An actuatable soft reservoir modulates host foreign body response , Sci. Robotics , 4 , (33 ), 2019, peaax7043 - Journal Article, 2019 URL DOI

Solazzo, M., Wong S., Zhussupbekova A., Krukiewicz K., Fleisher K., Biggs M. J. P., Monaghan, M. G. , Engineered electroconductive biomaterial platforms for cardiac tissue engineering applications, Sir Bernard Crossland Symposium 2019, Belfast, 17-18th April, 2019 Oral Presentation, 2019

Neto N., Greaney C., Olvera D., Duffy C., Hoey D., Monaghan M.G, Discerning the Effect of Substrate directed differentiation on Mesenchymal Stromal Cell Metabolic Activity using FLIM, 25th Bioengineering in Ireland 2019, , Limerick Ireland, 18-19th January, 2019 Oral Presentation, 2019

Solazzo M, Krukiewicz K, Zhussupbekova A, Fleischer K, Biggs MJ, Monaghan MG., PEDOT:PSS interfaces stabilised using a PEGylated crosslinker yield improved conductivity and biocompatibility., J Mater Chem B, 7, (31), 2019, p4811-4820 Journal Article, 2019 TARA - Full Text DOI

Solazzo, M., Zhussupbekova A., Krukiewicz K., Fleisher K., Biggs M. J. P., Monaghan, M. G, Minimally invasive 3D electroconductive biomaterial platforms for cardiac tissue engineering applications, Tissue Engineering and Regenerative Medicine International Society (TERMIS) EU Chapter meeting, Rhodes, Greece, 27- 31st of May, 2019 Oral Presentation, 2019

Shen N, Riedl JA, Carvajal-Berrio DA, Davis Z, Monaghan MG, Layland SL, Hinderer S, Schenke-Layland K. , A flow bioreactor system compatible with real-time two-photon fluorescence lifetime imaging microscopy., Biomedical Materials, 13, 2018, p024101- Journal Article, 2018 DOI

Solazzo, M., Monaghan, M. G, An electroconductive 3D porous PEDOT:PSS scaffold for cardiac tissue engineering applications, 24th Bioengineering in Ireland 2018, Dublin, 26-27th January, 2018 Oral Presentation, 2018

Monaghan M.G, Holeiter M, Brauchle E, Layland S.L, Lu Y, Deb A, Pandit A, Nsair A, Schenke-Layland K, Exogenous miR-29B delivery via a hyaluronan-based injectable system yields functional maintenance of the infarcted myocardium, Tissue Engineering Part A, 24, 2018, p57 - 67 Journal Article, 2018 DOI

Neto N., Greaney C., Olvera D., Duffy C., Hoey D., Monaghan M.G, Discerning the Effect of Substrate directed differentiation on Mesenchymal Stromal Cell Metabolic Activity using FLIM, Matrix Biology Ireland Galway Meeting, Galway, Ireland, 21st November , 2018 Oral Presentation, 2018

Lakner P.H*, Monaghan M. G*, M, Möller Y, Olayioye M.A, Schenke-Layland K. , Applying a phasor approach analysis of multiphoton fluorescence lifetime imaging microscopy measurements to probe the metabolic activity of three-dimensional in vitro cell culture models., Nature Scientific Reports, 7, 2017, p42730- Journal Article, 2017 DOI TARA - Full Text

Lotz C, Schmid F.F, Oechsle E, Monaghan M, Walles H, Groeber-Becker F.K, A crosslinked collagen hydrogel matrix resisting contraction to facilitate full-thickness skin equivalents, ACS Appl. Mater. Interfaces, 9, 2017, p20417 - 20425 Journal Article, 2017 DOI

Lakner P, Möller Y, Olayioye M, Brucker S.Y, Schenke-Layland K, Monaghan M.G., Multiphoton Microscopy in the Biomedical Sciences XVI, Proc SPIE9712, SPIE BioS Multiphoton Microscopy in the Biomedical Sciences XVI, San Francisco, 14th March 2016, edited by Ammasi Periasamy; Peter T. C. So; Karsten König , 2016, pp97120X Conference Paper, 2016 DOI

Molina-Arce C, Brauchle E, Monaghan M.G, Holeiter M, Schenke-Layland K. , Characterization of extracellular matrix in murine scar tissues with near infrared Raman microspectroscopy, Innovation Match MX, Guadalajara, Mexico, 6-8th April , 2016 Oral Presentation, 2016

Brauchle E, Knopf A, Bauer H, Shen N, Linder S, Monaghan MG, Ellwanger K, Layland SL, Brucker SY, Nsair A, Schenke-Layland K, Non-invasive Chamber-Specific Identification of Cardiomyocytes in Differentiating Pluripotent Stem Cells., Stem cell reports, 6, (2), 2016, p188-99 Journal Article, 2016 DOI

Monaghan MG, Kroll S, Brucker SY, Schenke-Layland K, Enabling Multiphoton and Second Harmonic Generation Imaging in Paraffin-Embedded and Histologically Stained Sections., Tissue engineering. Part C, Methods, 22, (6), 2016, p517-23 Journal Article, 2016 DOI

Lakner P, Möller Y, Olayioye M, Brucker S.Y, Schenke-Layland K, Monaghan, M.G, A Phasor approach analysis of multiphoton FLIM measurements of three-dimensional cell culture models, SPIE Photonics West meeting , San Francisco, USA, 13-18th February , 2016 Oral Presentation, 2016

Monaghan M.G, Holeiter M, Layland S.L, Schenke-Layland K, Cardiomyocyte generation from somatic sources " current status and future directions , Current Opinion in Biotechnology , 40, 2016, p49 - 55 Journal Article, 2016 DOI

Monaghan MG, Linneweh M, Liebscher S, Van Handel B, Layland SL, Schenke-Layland K, Endocardial-to-mesenchymal transformation and mesenchymal cell colonization at the onset of human cardiac valve development., Development (Cambridge, England), 143, (3), 2016, p473-82 Journal Article, 2016 DOI

Dash, B.C., Thomas, D., Monaghan, M., Carroll, O., Chen, X., Woodhouse, K., O'Brien, T., Pandit, A., An injectable elastin-based gene delivery platform for dose-dependent modulation of angiogenesis and inflammation for critical limb ischemia, Biomaterials, 65, 2015, p126-139 Journal Article, 2015 DOI

Monaghan M, Brauchle E, Carvajal Berrio D, Deb A, Pandit A, Nsair A, Schenke-Layland K, Biomaterial delivery of exogenous miR-29b to the infarcted myocardium yields improved myocardial recovery, the World Congress of the Tissue Engineering and Regenerative Medicine International Society, Boston, MA, USA, 8-11th September , 2015 Oral Presentation, 2015

Groeber, F., Engelhardt, L., Egger, S., Werthmann, H., Monaghan, M., Walles, H., Hansmann, J., Impedance Spectroscopy for the Non-Destructive Evaluation of In Vitro Epidermal Models, Pharmaceutical Research, 32, (5), 2015, p1845-1854 Journal Article, 2015 DOI

Browne, S., Monaghan, M.G., Brauchle, E., Berrio, D.C., Chantepie, S., Papy-Garcia, D., Schenke-Layland, K., Pandit, A., Modulation of inflammation and angiogenesis and changes in ECM GAG-activity via dual delivery of nucleic acids, Biomaterials, 69, 2015, p133-147 Journal Article, 2015 DOI

Optical reprogramming and opticalcharacterization of cells using femtosecond lasers in, editor(s)König K, Ostendorf A , Biomedical Applications in Optically-Induced Nanostructures for Biomedical and Technical Applications, De Gruyter , 2015, pp159 - 178, [1. Uchugonova A, Augspurger C, Monaghan M, Schenke-Layland K, Konig K] Book Chapter, 2015 URL

Schesny, M.K., Monaghan, M., Bindermann, A.H., Freund, D., Seifert, M., Eble, J.A., Vogel, S., Gawaz, M.P., Hinderer, S., Schenke-Layland, K., Preserved bioactivity and tunable release of a SDF1-GPVI bi-specific protein using photo-crosslinked PEGda hydrogels, Biomaterials, 35, (25), 2014, p7180-7187 Journal Article, 2014 DOI

Maurer M, Monaghan M, Möller Y, Olayioye M, Schenke-Layland K, Single-cell transfection within a 3D tumor model using optoporation, Biomedical Engineering Society Annual Meeting, San Antonio, Texas, USA, 22-25th October, 2014 Oral Presentation, 2014

Monaghan, M., Augspurger, C., Brauchle, E., Lakner, P., Breunig, G., Konig, K., Schenke-Layland, K., Fluorescent live time imaging from pluripotency to differentiation in mouse embryonic stem cells reveals endogenous autofluorescence profiles, Journal of Tissue Engineering and Regenerative Medicine, 8, 2014, p300-301 Journal Article, 2014 DOI

Monaghan, Michael, Browne, Shane, Schenke-Layland, Katja, Pandit, Abhay, A Collagen-based Scaffold Delivering Exogenous MicroRNA-29B to Modulate Extracellular Matrix Remodeling, Molecular Therapy, 22, (4), 2014, p786-796 Journal Article, 2014 DOI

Monaghan M, Browne S, Schenke-Layland K, Pandit A, A Collagen-based Scaffold Delivering Exogenous microRNA-29B to Modulate Extracellular Matrix Remodeling , Meeting of the German Matrix Biology Society (DGMB), Regensburg, Germany, 20-24th March , 2014 Oral Presentation, 2014

Michael Monaghan, An injectable collagen scaffold delivering exogenous microRNA as a therapy to modulate extracellular matrix remodelling , NUI Galway, 2013 Thesis, 2013 URL

Energy Regeneration systems in cell free protein synthesis in vitro in, editor(s)Bethaz C, Li Puma V , New Research on Protein Synthesis, Nova Science Publishers, Inc, 2013, pp67 - 76, [2. Kahlig A, Schwedhelm I, Monaghan M, Thein M, Hansmann J] Book Chapter, 2013 URL

Monaghan M, Browne S, Wang W, Pandit A, Delivery and efficacy of interfering RNA via a crosslinked collagen hydrogel in an excisional in vivo model and a human pre‐clinical model to reduce fibrosis, 3rd International Conference , Wurzburg, Germany, 23-25th May, 2012 Oral Presentation, 2012

Monaghan, M., Greiser, U., Wall, J.G., O'Brien, T., Pandit, A., Interference: An alteRNAtive therapy following acute myocardial infarction, Trends in Pharmacological Sciences, 33, (12), 2012, p635-645 Journal Article, 2012 DOI

Monaghan M, Browne S, Wang W, Pandit A, Delivery and efficacy of interfering RNA via a crosslinked collagen hydrogel in an excisional in vivo model and a human pre‐clinical model to reduce fibrosis, World Congress of Tissue Engineering and Regenerative Medicine International Society, Vienna, Austria, 5-8th September, 2012 Oral Presentation, 2012

Monaghan, M., Pandit, A., RNA interference therapy via functionalized scaffolds, Advanced Drug Delivery Reviews, 63, (4), 2011, p197-208 Journal Article, 2011 DOI

Monaghan M, Greiser U, Cao H, Wang W, Pandit A, A ligand enhanced dendritic PEGylated poly (2-(dimethylamino) ethyl diacrylate) as a vehicle of microRNA, Drug Delivery and Translational Research, 2, 2011, p406 - 414 Journal Article, 2011 DOI

Monaghan M, Browne S, Wang W, Pandit A, Scaffold-mediated non-viral inhibition of collagen type I and type III in cardiac fibroblasts, European Society of Biomaterials Conference, Dublin, Ireland, 4-9th September , 2011 Oral Presentation, 2011

Monaghan, M. Browne, S. Wang, W. Pandit, A.(ed.), Scaffold mediated non-viral inhibition of collagen type I and type III in cardiac fibroblasts, 2011 Proceedings of a Conference, 2011 URL

Monaghan, M. Holladay, C. Pandit, A.(ed.), A crosslinked collagen type i biomaterial reservoir for non-viral gene delivery in vivo, 2011 Proceedings of a Conference, 2011

Monaghan M, Mathew A, Greiser U, Wang W, Pandit A, Scaffold mediated delivery of microRNA immunopolyplexes, European Society of Biomaterials Conference, Tampere, Finland, 11-15th September, 2010 Oral Presentation, 2010

Mathew A, Monaghan M, Wang W, Pandit A. , A linear-dendritic hybrid poly (2-(dimethylamino) ethyl methacrylate) as a highly effective gene delivery vector, the European Society of Biomaterials Conference, Tampere, Finland, 11-15th September, 2010 Oral Presentation, 2010

Dash B C, Mahor S, Monaghan M, Woodhouse K A, Pandit A, Elastin like protein based hollow spheres for gene delivery, Tissue Engineering and Regenerative Medicine Conference, Galway, Ireland, 14-17th June, 2010 Oral Presentation, 2010

Monaghan M, Mathew A, Greiser U, Wang W, Pandit A, Scaffold mediated delivery of microRNA immunopolyplexes, Tissue Engineering and Regenerative Medicine Conference, Galway, Ireland, 14-17th June, 2010 Oral Presentation, 2010

Dash B.C, Mahor S, Monaghan M, Woodhouse K.A, Pandit A, Elastin like protein based hollow spheres for gene delivery, European Society of Biomaterials Conference, Tampere, Finland, 11-15th September, 2010 Oral Presentation, 2010

Kelly J.L, Doody J, Zeugolis D, Monaghan M, Kelly G, Devocelle M, Kilcoyne M, Joshi L, Pandit A, Collagen fibers modified with polysialic mimetic peptide are a suitable material for use as a synthetic peripheral nerve graft, Irish Association of Plastic Surgery Meeting, Dublin, IReland, 26th November, 2010 Oral Presentation, 2010

Dash, B.C., Réthoré, G., Monaghan, M., Fitzgerald, K., Gallagher, W., Pandit, A., The influence of size and charge of chitosan/polyglutamic acid hollow spheres on cellular internalization, viability and blood compatibility, Biomaterials, 31, (32), 2010, p8188-8197 Journal Article, 2010 DOI PURL

Matthew A, Réthoré G, Dash B C, Monaghan M, Pandit, A, Chitosan/polyglutamic acid nanospheres: DNA encapsulation and release, cellular uptake and blood interaction studies, UK Society for Biomaterials Conference, Antrim, UK, 2009 Oral Presentation, 2009

Réthoré G, Monaghan M, Dash B C, Pandit A, Chitosan-polyglutamic acid biodegradable nanospheres show a blood compatibility response that is dictated by size and surface charge, 2nd World Congress of the Tissue Engineering and Regenerative Medicine International Society, Seoul, Republic of Korea, 11-15th September, 2009 Oral Presentation, 2009

Non-Peer-Reviewed Publications

Meenakshi Suku, Lesley Forrester, Manus Biggs, Michael Monaghan, Tuning macrophage polarization to model myocardial infarction in the generation of functional cardiac organoids, 28th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Enfield Ireland, January 2023, 2023 Conference Paper, 2023

Sara Corbezzolo, Meenakshi Suku, Locke Davenport Huyer, Milica Radisic Michael G. Monaghan, Melt electrowriting of synclastically curved patches compensating the mechanical anisotropy of the infarcted myocardium, 28th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Enfield, Ireland, January 2023, 2023 Conference Paper, 2023

Monaghan Michael, MXene and Conductive Polymers in Tissue Engineering, Joint 2D Nanomaterials meeting between RCD and Royal College of Surgeons in Ireland, RCSI, Dublin, Ireland, October 2022, 2022 Invited Talk, 2022

Michael Monaghan, Engineering Non-invasive Tools to Profile Cell Metabolism in Differentiation and Disease, Tissue and Cell Engineering Society (TCES) Annual Meeting (joint with the EPSRC Funded Centres for Doctoral Training, Birmingham, UK, 14th June 2022, 2022 Invited Talk, 2022

Michael Monaghan, MXene and Conductive Polymers for Tissue Engineering, Ghent Bioengineering Research Seminar, Ghent, Belgium , October 2022, 2022, Ruslan Dmitriev Invited Talk, 2022

O'Rourke S., Neto N., Devilly E., Shanley L., Dunne A., Monaghan M. G, ., Immunometabolism in atherosclerosis: Investigating cholesterol crystals as potent drivers of metabolic reprogramming and M1 macrophage polarisation, 34th Conference of the European Macrophage and Dendritic Cell Society, Online, 24-25th June , 2021 Meeting Abstract, 2021

Shanley L., Mahon O. R., O'Rourke S. A., Neto N., Monaghan M. G., Kelly D., Dunne A, Particle size alters macrophage metabolism: Implications for bone tissue engineering, 6th European Congress for Immunology Meeting, Online, 1-4th September , 2021 Meeting Abstract, 2021

Fitzgerald H., Williams D. G., Campbell N. K., Barry P, O'Rourke S. A. Neto N., Monaghan M. G., Nolan D. P., Dunne A, Trypanosoma brucei secreted aromatic ketoacids activate the Nrf2/HO-1 pathway and suppress pro-inflammatory responses in primary murine and human immune cells, . 34th Conference of the European Macrophage and Dendritic Cell Society. Virtual conference , Online, 24-25th June, 2021 Meeting Abstract, 2021

Shanley L., Mahon O. R., O'Rourke S. A., Neto N., Monaghan M. G., Kelly D., Dunne A, Particle size alters macrophage metabolism: Implications for bone tissue engineering, 34th Conference of the European Macrophage and Dendritic Cell Society, Online, 24-25th June , 2021 Meeting Abstract, 2021

O'Rourke S., Neto N., Devilly E., Shanley L., Dunne A., Monaghan M. G, Immunometabolism in atherosclerosis: Investigating cholesterol crystals as potent drivers of metabolic reprogramming and M1 macrophage polarisation, 6th European Congress for Immunology Meeting, Online, 1-4th September , 2021 Meeting Abstract, 2021

Suku. M., Forrester. L., Biggs. M, Monaghan, M. G, Tuning of Macrophage Polarization in the generation of functional cardiac organoids, EPSRC-SFI Joint Student Day, Online, 7th September, 2021 Oral Presentation, 2021

O'Rourke S., Neto N., Devilly E., Shanley L., Dunne A., Monaghan M. G, Inflammation in atherosclerosis: Investigating cholesterol crystals as potent drivers of metabolic reprogramming and M1 macrophage polarisation, Termis Workshop: Cell-Matrix Interaction and Mechanobiology in Regenerative Medicine, Online, 11-12th June, 2021 Oral Presentation, 2021

Monaghan MG, ELectroconductive Biomaterial Patches and Scaffolds to Match the Mechanical Anisotropy of Organ-specific Tissues, Beacon Mini Symposium, Ireland-Canada University Foundation, Online, 2021, 2021 Invited Talk, 2021

Monaghan MG, Applying novel design and non-invasive investigations to advance the fields of tissue engineering and optics, School of Pharmacy Seminar Series at TCD, Virtual, 2020, 2020, School of Pharmacy, TCD Invited Talk, 2020

O'Rourke S., Neto N., Dunne A., Monaghan M. G., , Immune modulation in atherosclerosis: Investigating cholesterol crystals as potent drivers of metabolic reprogramming and M1 macrophage polarisation, Drexel Immune Modulation and Engineering Symposium, , Philadelphia, U.S.A, 11-13th November , 2020 Oral Presentation, 2020

Neto N, O'Rourke S., Vishwanathan A., Dunne A., O'Farrelly C., Monaghan M. G., , Metabolic Profiling of Macrophage Polarization by Fluorescence Lifetime Imaging Microscopy, Immunometabolism Symposium, Dublin, 5th April , 2019 Oral Presentation, 2019

Solazzo, M.*. Monaghan, M. G, Engineered electroconductive biomaterial platforms for cardiac tissue engineering applications, TBSI Research Symposium 2019, Dublin, 4th September , 2019 Oral Presentation, 2019

Michael Monaghan, Non-invasive, nonlinear microscopy in the evaluation of extracellular matrix and cell metabolic activity, ORS Musculoskeletal Summer Symposium, Dublin, 6-7th July, 2017, Dvid Hoey Invited Talk, 2017

Monaghan M, Browne S, Wang W, Pandit A, Scaffold-mediated non-viral inhibition of collagen type I and type III in cardiac fibroblasts, World Conference in Regenerative Medicine, Leipzig, Germany, 2-4th November, 2012 Oral Presentation, 2012

Monaghan M, Pandit A, Development of a 3D co-culture model to interpret the biological response of vascular biomaterials in vitro, , National Centre for Biomedical Engineering Science Research Day, Galway, Ireland, Galway, Ireland, March , 2008 Meeting Abstract, 2008

Research Expertise

Description

The focus of the Monaghan lab (www.monaghanlab.com) is the development of novel biomaterial design-based strategies to regenerate or repair damaged tissues and restore biomechanical function using informed and optimised design approaches. Core Research Areas: . Cardiac tissue engineering and cell derivitisation from multi/pluripotent stem cell sources . Macrophage assessment and inclusion in advanced biomaterial culture platforms. . In silico predictive modelling and optimisation of biomaterial geometries . Electroconductive biomaterial synthesis, fabrication and applications . Advanced non-linear microscopy using multiphoton microscopy for non-invasive imaging of extracellular matrix . Advanced fluoresecent lifetime imaging microscopy (FLIM) in the assessment of cellular metabolism during disease, differentiation and regeneration. . Melt-electrowriting development, optimisation and application Our lab is leading new approaches for biomaterial fabrication and design using advanced biomaterials, tissue engineering, biofabrication and in silico predictive modelling. This workis creating a paradigm shift in which the scientific community, industry partners and clinical investigators approach biomaterial design. Our lab is recognised internationally as world class where we am regularly invited to deliver keynote talks at major international meetings, and our papers have received awards for being most downloaded in our field. Members of the team have also been awarded prizes at national/international level in recognition of the scientific quality of our labs work. I have coordinated and elevated the Med3DP initiative over the past five years (www.med3dp.com) which develops medical devices for healthcare using 3D printing technology, demonstrating research led teaching and student led learning. Our lab also has collaborative funding with small-medium enterprises such as Spraybase® (Bioprinting; next generation implantable devices) and I am a funded investigator in the SFI funded centres AMBER and CURAM. My work spans across and engages with several disciplines which includes Physics and Chemistry, Computer Science, Biochemistry and Immunology, Pharmacy, Surgery, Oncology and Endocrinology. This is strongly reflective in our illustrious repertoire of publications. Link to Google Scholar: https://scholar.google.com/citations?user=X1mgIbEAAAAJ&hl=en&oi=sra

Projects

  • Title
    • Engineering animal-free accurate models of the annulus fibrosis in vitro using morphometrical data applied to the 4D deposition of melt electrowritten fibres to achieve true anisotropy and lamellar orientations
  • Summary
    • Disease and debilitation of the intervertebral disc poses enormous burden on patient quality life and a significant cost in terms of clinical and economy costs. Tissue engineering is one promising approach to develop therapies for the intervertebral disc but requires the use of animal models to capture the mechanical environment of the intervertebral disc. This project will engineer, and 3D print an accurate in vitro model of the intervertebral disc and benchmark it with ex vivo ovine models which are standardly used in the field. This 3D printing technology offers a superior approach to achieve this; melt electrowriting will accurately capture the ellipsoidal geometry and alternating lamellar orientation of the annulus fibrosis. We will optimise the design and fabrication of this model using melt electrowriting and verify its suitability using mechanical testing and in vitro compatibility testing using human and sheep cells of the intervertebral disc. This approach will lead to the establishment of accurate in vitro models to optimise therapies of the intervertebral disc, minimise our reliance on animal models and lead to more `human-specific" models.
  • Funding Agency
    • Irish Research Council
  • Date From
    • Sept 2022
  • Date To
    • Dec 2023
  • Title
    • Enabling Melt ElectroWriting of Electroconductive, Geometrically Defined 3D Biomaterials to Promote the Maturation of Cardiomyocytes for Cardiac Regeneration
  • Funding Agency
    • Enterprise Ireland
  • Date From
    • December 2017
  • Date To
    • January 2020
  • Title
    • Doctoral Network for a Shared Excellence of Fluorescent Lifetime Imaging Microscopy in Biomedical Applications
  • Summary
    • In flIMAGIN3D, which centres on fluorescent lifetime imaging microscopy applied to biomedical platforms in 3D; ten talented Doctoral Candidates (DCs) will be recruited, trained and supervised to cohesively establish the most robust, user accessible and powerful FLIM-based platform-never achieved before, and apply it to a wide range of applications in the biological sciences. flIMAGIN3D will generate ten DCs)at the cutting edge of FLIM-based biological imaging who will spearhead, and go onto catalyse clusters of FLIM excellence across Europe; each intertwined within a collaborative network that will extend beyond the time-frame of the program. This doctoral network will train talented doctoral candidates in the essential theories underpinning FLIM , and through the individual projects and extensive secondments in this network; elevate their projects and skills, and that of their secondment hosts to research excellence in FLIM flIMAGIN3D addresses a significant knowledge gap in the field of photonics in biology by involving biomedical scientists in each and every aspect of development and design, and cognisant of the wide variety of current platforms that require adaptability to such modalities. In flIMAGIN3D: bringing together representatives with different applications and backgrounds, we will identify and overcome barriers to FLIM imaging by developing the next generation of scientists who will develop a FLIM platform capable of extracting the greatest amount of information possible in a stream-lined, accurate and user-friendly manner in order to understand biology in disease, differentiation, development and interventions at a high resolution. To achieve this goal, the trained scientists will develop cheaper advanced hardware components, with user friendly software that can build precise libraries for accurate machine learning classifications and plug-and-play platforms and sensors to facilitate a wide range of applications.
  • Funding Agency
    • European Commission
  • Date From
    • January 2023
  • Date To
    • December 2026
  • Title
    • Development of minimally invasive neuromodulatory electroconductive hydrogel in combination with physical stimuli for spinal cord regeneration
  • Summary
    • Spinal cord injury (SCI) is a debilitating health conditions affecting circa 27.04 million people globally including 6.4 million Europeans with serious impact on the quality of life and socio-economic status of the patients. Existing regenerative and rehabilitation approaches are largely palliative and neuroprotective, targeting the spared neural tissue after injury with limited efficacy in regeneration. While the spinal cord has poor regenerative ability, the degenerative processes further complicate the SCI pathophysiology. Hence, no regenerative and rehabilitation approaches, individually, have resulted in complete and near complete functional recovery to date. To fully restore the injured spinal cord's functionality, a coordinated combinational approach addressing particular degenerative processes is necessary. Herein, the fellow proposes to develop a regenerative rehabilitation combinational approach to maximize the functional regenerative outcome after SCI using minimally invasive injectable electroconductive hydrogels (ECHs) functionalized with neurotrophins (NTs) and anti-inflammatory cytokines in combination with electrical stimulation (ES) and near infrared (NIR) laser stimulation. ES and NIR laser through the ECH will be used to stimulate the neurons and glial cells to enhance regeneration and neuroplasticity as well as for on demand delivery of biologics to target the inhibitory processes. This proposal describes the project's multidisciplinary nature, execution and management of the project by the fellow and the multiple collaborators who will each provide the fellow with cutting-edge research skills. The goals of the fellow's project place specific focus to ensure that the training of the fellow brings him to a level of competitive scientific excellence at international level.
  • Funding Agency
    • European Commission
  • Date From
    • September 2021
  • Date To
    • August 2023
  • Title
    • The role of macrophage polarization in the generation of functional engineered mature myocardium organoids
  • Summary
    • It is the purpose of this PhD project to intertwine the fields of bioengineering, biochemistry and immunology, by combining macrophages; key cells of the innate immune system, with cardiac organoids to achieve a more physiologically relevant model and achieve a diseased 'heart-attack-on-a-dish' organoid model as a patient specific pharmacological testing platform.
  • Funding Agency
    • Trinity College Dublin Faculty of Engineering Maths and Sciences Intrafaculty PhD College Award
  • Date From
    • September 2018
  • Date To
    • August 2022
  • Title
    • Cell-Specific Metabolic FLIM Profiling of the Ulcerative Colitis Microenvironment to Establish an Association with Patient Treatment Response
  • Summary
    • Non-invasive FLIM could reliably establish a statistically significant relationship between the FLIM characteristics of UC biopsies and UC patient response. The specific aims of this research project are to employ FLIM to characterise the microenvironment of lesions in UC. This includes measurement of the mean fluorescent decay times, contributing lifetimes and ratios of NADH and FAD within cellular populations dominating the UC microenvironment in a spatio-temporal manner. This will pave the way towards launching a FLIM imaging core facility that can reliably categorise cell specific metabolic profiles within tissue biopsies from patients diagnosed with UC, and provide recommendations for treatment regimens.
  • Funding Agency
    • Trinity College Dublin Provosts PhD Project Award
  • Date From
    • September 2018
  • Date To
    • August 2022
  • Title
    • Feasibility and scope of PEDOT:PSS dry electrodes for Connected Health: Monitoring Fitness and Wellbeing"
  • Funding Agency
    • Enterprise Ireland
  • Date From
    • Dec 2022
  • Date To
    • May 2023
  • Title
    • CardiacEire: An Ireland-centered approach unifying stakeholders in research to address cardiac disease in young people
  • Summary
    • Sudden Adult Death Syndrome (SADS) is an event that devastates communities and families, often without any warning. Given the devastation of this disease whereby seemingly young healthy individuals die suddenly; there is a strong drive both nationally and internationally to uncover the causes of this, particularly when heart failure is the cause. Specific causes of heart-related SADS are known , however there is still much more to be done. This proposal aims to gather all interested parties in Ireland, from research labs, the clinic, affected families and governmental policy-makers to have a unified approach as international players addressing this disease.
  • Funding Agency
    • Irish Research Council- New Foundations
  • Title
    • Recapitulating electrical, mechanical and extracellular-matrix cues via multifunctional biomaterials to engineer cardiac tissue
  • Funding Agency
    • SFI-HRB-Wellcome Trust
  • Date From
    • January 2017
  • Date To
    • Septebmer 2019
  • Title
    • 3D Electroconductive biomaterial scaffolds as cardioinductive matrices for cardiac tissue engineering
  • Summary
    • Heart attacks lead to the death of functional cells in heart muscle (cardiomyocytes; CMs) which cannot be replaced. Every sixth man and seventh woman will die from a heart attack or related complications, and there is no way to regenerate this heart muscle. CMs in vivo are exposed to a unique environment defined by the local extracellular matrix (ECM), dynamic mechanical forces via the beating of the heart and electrical action potentials being generated by the heart's electrical conduction system. This proposal is a unique engineering approach to recapitulate a cardio-inductive platform based on mechanical, electrical and ECM cues to generate an efficient culture system to culture CMs in vitro and also to increase efficiencies of generating functional CMs from progenitor cells. This research aims to generate the following outcome: a cardio-inductive biomaterial system that has appropriate mechanical electroconductive and biocompatibility properties that can facilitate the generation of cardiac organoids in vitro using primary cells and efficiently facilitate cardiomyogenic differentiation from progenitor cells. These advances will not only improve quality of life for the patient but provide tools to study disease and perform pharmaceutical research.
  • Funding Agency
    • Irish Research Council
  • Date From
    • 01-SEP-19
  • Date To
    • 31-AUG-21
  • Title
    • bioBRILLOUIN EI Support
  • Funding Agency
    • Enterprise Ireland
  • Date From
    • July 2022
  • Date To
    • Dec 2022
  • Title
    • Conductive materials for regeneration of the infarcted myocardium
  • Summary
    • Every sixth man and seventh woman will die from a heart attack or related complications Heart attacks lead to the death of functional cells in heart muscle (cardiomyocytes; CMs) which cannot be replaced. CMs in vivo are exposed to a unique environment defined by the local extracellular matrix (ECM), dynamic mechanical forces via the beating of the heart and electrical action potentials being generated by the heart's electrical conduction system. Electroconductive biomaterials in heart tissue engineering have the potential to be a building block for advanced therapeutic medical devices and also to achieve functional cardiac tissue in vitro. This project is a unique materials engineering approach to develop a cardio-inductive platform based on mechanical, electrical and ECM cues to generate the next generation of biomaterials. This research aims to generate the following outcome: a cardio-inductive biomaterial system derived from advanced nanomaterials that has appropriate mechanical, electroconductive and biocompatibility properties that can facilitate the generation of cardiac organoids in vitro using primary cells and efficiently facilitate cardiomyogenic differentiation from progenitor cells. These advances will not only improve quality of life for patients, but also provide tools to study disease and perform pharmaceutical research. This PhD program will develop a 3D biomaterial scaffold using synthetic nanomaterials together with naturally derived ECM that is biocompatible, suitably electroconductive and cardio-inductive towards facilitating the culture of cardiac organoids in vitro and enhancing stem cell cardiomyogenesis in vitro. Methods will include materials chemical characterisation techniques, cell culture, biochemical assays, mechanical testing, stem cell culture, immunohistochemistry, instrumentation. Support and training are provided throughout all stages.
  • Funding Agency
    • SFI
  • Date From
    • 01-Oct-19
  • Date To
    • 31-Sep-23
  • Title
    • Tuning macrophage polarization to model myocardial infarction in the generation of functional cardiac organoids'
  • Summary
    • It is the purpose of this PhD project to intertwine the fields of bioengineering, biochemistry and immunology, by combining macrophages; key cells of the innate immune system, with cardiac organoids to achieve a more physiologically relevant model and achieve a diseased 'heart-attack-on-a-dish' organoid model as a patient specific pharmacological testing platform. The generation of cardiac organoids can be achieved by recapitulating, as close as possible, the native microenvironment of the myocardium. Therefore, conductive polymers have gained interest as biomaterials for cardiac tissue engineering due to their suitable conductivity properties4. Very few reports have explored the interaction of macrophages and electroconductive biomaterials, and there are no reports regarding their introduction into cardiac organoids, or recapitulation of disease in a dish models
  • Funding Agency
    • EPSRC-SFI Joint Funding
  • Date From
    • 01-Oct-19
  • Date To
    • 31-Sep-23
  • Title
    • Enhancement of an Olympus FV1000MPE multiphoton microscope in Trinity Biomedical Sciences Institute (TBSI) for the application of Fluorescent Lifetime Imaging Microscopy (FLIM)
  • Summary
    • Enhancement of an Olympus FV1000MPE multiphoton microscope in Trinity Biomedical Sciences Institute (TBSI) for the application of Fluorescent Lifetime Imaging Microscopy (FLIM)
  • Funding Agency
    • SFI Funding internally allocated by TCD
  • Date From
    • 01-Feb-17
  • Date To
    • N/a
  • Title
    • Research Boost Program
  • Funding Agency
    • TCD Dean of Research
  • Date From
    • November 2020
  • Date To
    • September 2021
  • Title
    • Materials in Tissue Engineering
  • Funding Agency
    • International Association for the Exchange of Students for Technical Experience (IAESTE)
  • Date From
    • 01/06/2019
  • Date To
    • 31/08/2019
  • Title
    • Coordination Support - ERC version
  • Funding Agency
    • Enterprise Ireland
  • Date From
    • April 2022
  • Date To
    • March 2023
  • Title
    • Resist-AMR Antimicrobial Resistance: Engineering Natural, One- Health, Systems Thinking Solutions to a Manmade Global Disaster
  • Summary
    • Antimicrobials are critical resources for human, animal and plant health. With emergence of antimicrobial resistance and lack of new antimicrobials, we face an unprecedented global environmental, food security and human health threat. Applying a multidisciplinary approach, the team including 4 PhD projects and expertise from plant scientists, clinical and environmental microbiologists, geneticists, antimicrobial resistance specialists, computer scientists/statisticians, bioengineers and sociologists, will study environmental and human `resistomes" from agricultural and clinical settings and analyse agricultural stakeholders" practices and policies to identify institutional reform implications.
  • Funding Agency
    • TCD Alumni
  • Date From
    • March 2023
  • Date To
    • Feb 2027

Keywords

2ND HARMONIC GENERATION; 3D Imaging Analysis; Bioengineering and radiologic imaging; Biomaterials; COLLAGEN; CONFOCAL IMAGING; Confocal photoluminescence imaging; DRUG TARGETING; DRUG-DELIVERY SYSTEM; EXTRACELLULAR MATRIX (ECM); GENE SILENCING; GENE-THERAPY; Regenerative Medicine; Tissue Engineering

Recognition

Representations

Elected Treasurer- Matrix Biology Ireland 2017 Meeting 30 Nov 2016

Elected Council Member -Tissue Engineering and Regenerative Medicine International Society (EU Chapter) 01-Jan-2018

Reviewer for leading journals in the fields of Biomaterials and Tissue Engineering (selection only): Biomaterials, RSC Biomaterials Science, Advanced Optical Materials, ACS Biomaterials Science and Engineering, Macromolecular Materials and Engineering, Small, Macromolecular Bioscience, Advanced Functional Materials, Advanced NanoBiomed Research, Advanced Materials Technologies, Journal of Controlled Release, Molecular Therapy, Advanced Healthcare Materials, Tissue Engineering Part A, Tissue Engineering Part B, Acta Biomaterialia, ACS Applied Materials and Interfaces, Advanced Drug Delivery Reviews, Lab on a Chip, Nature Scientific Reports, Journal of Tissue Engineering and Regenerative Medicine, npj 2D Materials and Applications 2015-Present

External Examiner PhD Thesis Progress- Royal College of Surgeons in Ireland 2017

Grant Reviewer for National and International Granting Bodies Including: European Research Council ADVANCED GRANTS European Research Council Starting Grants Wellcome Trust (2020-Present) MRC-AMED Regenerative Medicine and Stem Cell Research Initiative (2020- Present) Israel Science Foundation (2020-Present) Marie Sklowdowska-Curie Individual Fellowships (2019-Present) Freiburg Institute for Advanced Studies Deutsche Forschungsgemeinschaft (DFG) (2029-Present) Canada Foundation for Innovation (CFI) through the John R. Evans Leaders Fund (2021) 2018

Awards and Honours

Senior author on paper awarded the 'Engineers Ireland Biomedical Research Medal' from the Society of Engineers Ireland. Awarded to Dr Matteo Solazzo 2022

European Doctoral Award, Issued by the European Society of Biomaterials 2015

Deutscher Akademischer Austausch Dienst, Short Term Travel Award to conduct research in University Hospital Tübingen 2012

EMBO Short Term Travel Award to conduct research in Fraunhofer IGB Stuttgart 2011

Roche Best Poster Award, NCBES Research day 2008

NUI Galway College of Engineering and Informatics Research Fellow 2007

University Scholar (Based on end of term results) NUI Galway 2005

Memberships

Royal Academy of Medicine in Ireland (RAMI)- Bioengineering Section 2017 – Present

MBI- Matrix Biology Ireland (elected council member and Society Treasurer) 2016 – Present

ESB- European Society for Biomaterials 2010 – Present

TERMIS - Tissue Engineering and Regenerative Medicine Society 2009 – Present

DGMB-German Matrix Biology Society 2013 – 2016

Funded Investigator: SFI Funded Centre AMBER (Advanced Materials and BioEngineering Research) 2019 – Present

Funded Investigator: SFI Funded Centre CÚRAM (Designing The Next Generation of 'Smart' Medical Devices) 2019 – Present