Common Fluorescent Probes

Spectral properties of similar dyes

Dye Name	Colour	Excitation Maxima	Emission Maxima
Hoechst/DAPI	Blue	350 nm	461 nm
ECFP	Cyan	439 nm	476 nm
EGFP	Green	484 nm	507 nm
Alexa 488	Green	495 nm	519 nm
FITC	Green	495 nm	521 nm
DyLight 488	Green	493 nm	518 nm
Cy2 dye	Green	492 nm	510 nm
EYFP	Yellow	514 nm	527 nm
DyLight 549	Yellow	562 nm	576 nm
Alexa 555	Orange	555 nm	565 nm
Cy3 dye	Orange	550 nm	570 nm
Tetra Methyl Rhodamine (TRITC)	Orange	550 nm	570 nm
Alexa 546	Orange	556 nm	573 nm
TagRFP	Red-Orange	555 nm	584 nm
Rhodamine RedX	Red-Orange	570 nm	590 nm
Alexa 555	Red-Orange	555 nm	565 nm
Alexa 568	Red-Orange	578 nm	603 nm
mRFP1	Red-Orange	584 nm	607 nm
DyLight 594	Red-Orange	593 nm	613 nm
Alexa 594	Red	590 nm	617 nm
DyLight 594	Red	591 nm	616 nm
Texas Red	Red	596 nm	620 nm
Alexa 610	Red	612 nm	628 nm
Alexa 633	Far Red	632 nm	647 nm
Alexa 647	Far Red	650 nm	668 nm
Cy5	Far Red	649 nm	670 nm
DyLight 649	Far Red	652 nm	670 nm