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You are here Research > Cell Analysis Facilities > Flow Cytometry Facility > Instrumentation

Instrumentation

The Flow Cytometry Facility is equipped with one flow sorter, one imaging cytometer, four analysers, an in vivo fluorescence & luminescence imager (Biospace Lab PhotonIMAGER RT), an Automated Cell Counter (Luna Cell Counter), and a fluorescence microscope (Partec Cyscope).

BD FACSAria Fusion Cell Sorter:

View the machine on the BD Biosciences website.

BD FACSAria Fusion Cell Sorter

The BD FACSAria Fusion High Performance Cell Sorter combines a top of the range high speed cell sorter with best-in-class biosafety. Housed in a Class II Type A2 biosafety cabinet, the sorter performs with unrivalled sensitivity and resolution. With 4 nozzle sizes (70, 85, 100, & 130 µm), 4 lasers (405, 488, 561, & 640 nm) and 16 fluorescence parameters, this provides a vast array of options for the researcher.

The sorter is capable of up to 4 sorts simultaneously into a variety of different receptacles. Also, single cell deposition into most standard 80mm x 120 mm multi-well tray formats (e.g. 96-well plate) and onto standard slides is routine with the ACDU system. Both sample and collection tubes can be kept at a fixed temperature, which leads to maximal viability.

To view fluorescence channels go to Fluorochromes tab.

Amnis ImageStream X Mk II:

View the machine on the Luminex website

Amnis ImageStream X Mk II

The Amnis ImageStream X Mark II, the first such instrument installed in Ireland, is a powerful platform that combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. At rates of thousands of cells per second, the instrument produces multiple high-resolution images of each individual cell directly in flow, with up to 10 fluorescent markers (405, 488, 561, 642 nm lasers) and sensitivity exceeding conventional flow cytometers. This unique technology enables a broad range of applications that cannot be achieved with existing instrumentation within conventional flow cytometry and confocal microscopy.

Imaging cytometry has a wide variety of applications, in particular:

• cell morphology
• cell signalling and molecular translocation
• co-localising and trafficking
• autophagy
• DNA damage & repair
• oncology
• microbiology
• internalisation
• cell-cell interactions
• rare cell investigation
• cell cycle & mitosis
• stem cell differentiation
• parasitology
• oceanography

In addition to the hardware, the platform is supported by powerful analysis software (IDEAS software), which combines image analysis, statistical rigour, and visual confirmation. Hundreds of parameters are calculated for every single cell, including fluorescence intensity, fluorescence localisation, cell shape, cell texture and numerous other morphologic and photometric features; while a wide range of statistical metrics reveal variations.

BD LSRFortessa:

View the machine on the BD Biosciences website.

BD LSRFortessa

Features include 4 lasers (405, 488, 561 & 640 nm) and 16 fluorescence parameters, with maximal sensitivity and resolution, and up to 40,000 events per second. To view fluorescence channels go to Fluorochromes tab.

BD FACSCanto II:

View the machine on the BD Biosciences website.

BD FACSCanto II

Features include 3 lasers (405, 488 & 633 nm) and 8 fluorescence parameters, with high sensitivity and resolution, and up to 10,000 events per second.

To view fluorescence channels go to Fluorochromes tab.

Beckman Coulter CyAn ADP:

Beckman Coulter CyAn ADP

Features include 3 lasers (405, 488 & 633 nm) and 9 fluorescence parameters, with high sensitivity and resolution, and up to 50,000 events per second.

To view fluorescence channels go to Fluorochromes tab.

BD Accuri C6:

View the machine on the BD Biosciences website.

BD Accuri C6

Features include 2 lasers (488 & 633 nm) and 4 fluorescence parameters, with high sensitivity and resolution, and up to 10,000 events per second.

To view fluorescence channels go to Fluorochromes tab.