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Cell Analysis Facilities

The state-of-the-art Trinity Cell Analysis Technologies in the School of Biochemistry and Immunology is a consolidated research technology service, incorporating world-class infrastructure and facilities.
It is led by the Director of Research Technology (Dr Barry Moran) and includes cytometry, histology, microscopy, imaging and analytical instrumentation, with associated expertise. Facilities include the Flow Cytometry Facility, the Microscopy and Imaging Facility, and the Biological Transmission Electron Microscopy (bioTEM) Facility. The cores are housed in custom-built laboratory space within the Trinity Biomedical Sciences Institute, each with an experienced manager. Additional specialist technologies in the School include Seahorse instrumentation, Mabtech FluoroSpot and ELISpot, the Samplix Xdrop microfluidic encapsulating system, as well as the Agilent Ultra High Field 800 MHz Nuclear Magnetic Resonance (NMR) system.

Flow Cytometry Facility

Flow cytometry is a high-speed, high-parameter single cell analysis technology. The Flow Cytometry Facility, established in 2005, is the best equipped cytometry core in Ireland. The facility houses best-in-class instrumentation, including a cell sorter (BD FACSAria Fusion), an imaging cytometer (Amnis ImageStream X Mark II), a full-spectrum cytometer (Cytek Aurora) and four conventional flow cytometers (BD LSRFortessa, BD FACSCanto II, BD Accuri C6 & Agilent’s Advanteon).

The lab is available to all researchers and serves over 130 research groups, with ~ 200 regular users per month. The facility manager, Dr Barry Moran, has considerable experience and expertise and works with researchers in all aspects of their cytometry projects, from experimental & panel design, to data acquisition, onto analysis and presentation.

Microscopy and Imaging Centre

The Microscopy and Imaging Facility within the School of Biochemistry and Immunology supports histology and light microscopy technologies within the school, and its instrumentation and services are available to all researchers. The instruments include STED, confocal, spinning disk, upright and invertedmicroscopes, as well as histology preparation tools. The facility manager has considerable experience and expertise, working with researchers in all aspects of their imaging projects.

Biological Transmission Electron Microscopy (bioTEM) Facility

Biological Transmission Electron Microscopy (TEM) is a vital research tool, providing insight into the mechanisms of cells, tissue, organisms and bio-molecules. Biological TEM is indispensable in structural biology, virology, and materials science, complementing the other imaging and analytical techniques in the School. The facility features a JEOL JEM-1400 120Kv TEM, which is ideally suited to life sciences applications. The facility manager, Mr Neal Leddy, has considerable experience, providing expert support in experimental design, sample prep, instrument operation and image acquisition.

Brief introduction of the CL3 lab