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Frederick Sheedy
Ussher Assistant Professor, Biochemistry


I recently established my independent research group within the School of Biochemistry & Immunology at Trinity College, Dublin (TCD), Ireland, examining macrophage function in health and disease. I pioneered miRNA research in the field of innate immunity during my graduate studies in the O'Neill Lab, resulting in a Nature Immunology first author publication and this emerging field was highlighted by a Nature Reviews Immunology article, which I co-wrote. I obtained a postdoctoral fellowship from the American Heart Association to study the molecular mechanisms of inflammation in disease at NYU. This transformative work was published in Nature Immunology and alongside my recent work on tuberculosis, funded by a Starting Investigator grant from Science Foundation Ireland, fueled my interest in macrophage-driven disease. My recent work published in Cell Metabolism, J Immunology and AJRCMB (as last author), has uncovered the regulation of anti-microbial function by metabolism and through a partnership with Kerry Group, I have begun to examine the potential for β-glucan training of immunity. Obtaining SFI Frontier funding will establish myself as an international leader in the newly emerging field of Innate Immune Training.

Publications and Further Research Outputs

Peer-Reviewed Publications

Gleeson LE, O'Leary SM, Ryan D, McLaughlin AM, Sheedy FJ, Keane JM., Cigarette Smoking Impairs the Bioenergetic Immune Response to Mycobacterium Tuberculosis Infection., American journal of respiratory cell and molecular biology, 2018 Journal Article, 2018 DOI

Ni Cheallaigh Cliona, Sheedy Frederick J., Shields Up: Systemic Protection Provided by microRNA-21 During Sepsis?, 45 , (7 ), 2017 Journal Article, 2017 URL

Hackett, E.E., Sheedy, F.J., MiR-21 alters circulating Treg function in vascular disease-hope for restoring immunoregulatory responses in atherosclerosis?, Annals of Translational Medicine, 5, (1), 2017 Journal Article, 2017

Gleeson LE, Sheedy FJ, Palsson-McDermott EM, Triglia D, O'Leary SM, O'Sullivan MP, O'Neill LA, Keane J, Cutting Edge: Mycobacterium tuberculosis Induces Aerobic Glycolysis in Human Alveolar Macrophages That Is Required for Control of Intracellular Bacillary Replication., Journal of immunology (Baltimore, Md. : 1950), 196, (6), 2016, p2444-2449 Journal Article, 2016 DOI TARA - Full Text URL

Ní Cheallaigh, C., Sheedy, F.J., Harris, J., Muñoz-Wolf, N., Lee, J., West, K., McDermott, E.P., Smyth, A., Gleeson, L.E., Coleman, M., Martinez, N., Hearnden, C.H., Tynan, G.A., Carroll, E.C., Jones, S.A., Corr, S.C., Bernard, N.J., Hughes, M.M., Corcoran, S.E., O'Sullivan, M., Fallon, C.M., Kornfeld, H., Golenbock, D., Gordon, S.V., O'Neill, L.A., Keane, J. Lavelle, E.C, A Common Variant in the Adaptor Mal Regulates Interferon Gamma Signaling., Immunity, 44, (2), 2016, p368 - 379 Journal Article, 2016 TARA - Full Text URL

Gleeson, L.E., Sheedy, F.J., Metabolic reprogramming & inflammation: Fuelling the host response to pathogens, Seminars in Immunology, 28, (5), 2016, p450-468 Journal Article, 2016

Ouimet, M., Koster, S., Sakowski, E., Ramkhelawon, B., Van Solingen, C., Oldebeken, S., Karunakaran, D., Portal-Celhay, C., Sheedy, F.J., Ray, T.D., Cecchini, K., Zamore, P.D., Rayner, K.J., Marcel, Y.L., Philips, J.A., Moore, K.J., Mycobacterium tuberculosis induces the MIR-33 locus to reprogram autophagy and host lipid metabolism, Nature Immunology, 17, (6), 2016, p677-686 Journal Article, 2016

Sheedy, F.J., Turning 21: Induction of miR-21 as a key switch in the inflammatory response, Frontiers in Immunology, 6, (JAN), 2015 Journal Article, 2015 TARA - Full Text

Ouimet, M., Ediriweera, H.N., Mahesh Gundra, U., Sheedy, F.J., Ramkhelawon, B., Hutchison, S.B., Rinehold, K., Van Solingen, C., Fullerton, M.D., Cecchini, K., Rayner, K.J., Steinberg, G.R., Zamore, P.D., Fisher, E.A., Loke, P., Moore, K.J., MicroRNA-33-dependent regulation of macrophage metabolism directs immune cell polarization in atherosclerosis, Journal of Clinical Investigation, 125, (12), 2015, p4334-4348 Journal Article, 2015

Palsson-McDermott EM, Curtis AM, Goel G, Lauterbach MA, Sheedy FJ, Gleeson LE, van den Bosch MW, Quinn SR, Domingo-Fernandez R, Johnson DG, Jiang J, Israelsen WJ, Keane J, Thomas C, Clish C, Vanden Heiden M, Xavier RJ, O'Neill LA, Pyruvate Kinase M2 Regulates Hif-1alfa Activity and IL-1ß Induction and Is a Critical Determinant of the Warburg Effect in LPS-Activated Macrophages., Cell metabolism, 21, (1), 2015, p65-80 Journal Article, 2015 DOI TARA - Full Text

Ramkhelawon, B., Hennessy, E.J., Ménager, M., Ray, T.D., Sheedy, F.J., Hutchison, S., Wanschel, A., Oldebeken, S., Geoffrion, M., Spiro, W., Miller, G., McPherson, R., Rayner, K.J., Moore, K.J., Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity, Nature Medicine, 20, (4), 2014, p377-384 Journal Article, 2014

Fernandez-Hernando C, Moore KJ, Rayner KJ, Sheedy FJ, Suarez Y, 'miR-33 inhibitors and uses thereof to decrease inflammation', US Patent and Trading Office, PCT/US2012/035855 , 2014 Patent, 2014 URL

Sheedy, F.J., Grebe, A., Rayner, K.J., Kalantari, P., Ramkhelawon, B., Carpenter, S.B., Becker, C.E., Ediriweera, H.N., Mullick, A.E., Golenbock, D.T., Stuart, L.M., Latz, E., Fitzgerald, K.A., Moore, K.J., CD36 coordinates NLRP3 inflammasome activation by facilitating intracellular nucleation of soluble ligands into particulate ligands in sterile inflammation, Nature Immunology, 14, (8), 2013, p812-820 Journal Article, 2013

Sheedy, F.J., Moore, K.J., IL-1 signaling in atherosclerosis: Sibling rivalry, Nature Immunology, 14, (10), 2013, p1030-1032 Journal Article, 2013

Moore, K.J., Sheedy, F.J., Fisher, E.A., Macrophages in atherosclerosis: A dynamic balance, Nature Reviews Immunology, 13, (10), 2013, p709-721 Journal Article, 2013

O'Neill, L.A., Sheedy, F.J., McCoy, C.E., MicroRNAs: The fine-tuners of Toll-like receptor signalling, Nature Reviews Immunology, 11, (3), 2011, p163-175 Journal Article, 2011

Hennessy EJ, Sheedy FJ, Santamaria D, Barbacid M, O'Neill LA., Toll-like receptor 4 (TLR4) down-regulates microRNA-107 increasing macrophage adhesion via cyclin-dependent kinase 6, Journal of Biological Chemistry, 286, (29), 2011, p25531-9 Journal Article, 2011 TARA - Full Text DOI URL

Rayner, K.J., Sheedy, F.J., Esau, C.C., Hussain, F.N., Temel, R.E., Parathath, S., Van Gils, J.M., Rayner, A.J., Chang, A.N., Suarez, Y., Fernandez-Hernando, C., Fisher, E.A., Moore, K.J., Antagonism of miR-33 in mice promotes reverse cholesterol transport and regression of atherosclerosis, Journal of Clinical Investigation, 121, (7), 2011, p2921-2931 Journal Article, 2011

Rayner, K.J., Esau, C.C., Hussain, F.N., McDaniel, A.L., Marshall, S.M., Van Gils, J.M., Ray, T.D., Sheedy, F.J., Goedeke, L., Liu, X., Khatsenko, O.G., Kaimal, V., Lees, C.J., Fernandez-Hernando, C., Fisher, E.A., Temel, R.E., Moore, K.J., Inhibition of miR-33a/b in non-human primates raises plasma HDL and lowers VLDL triglycerides, Nature, 478, (7369), 2011, p404-407 Journal Article, 2011

Durrani O, Banahan K, Sheedy FJ, McBride L, Ben-Chetrit E, Greiner K, Vaughan RW, Kondeatis E, Hamburger J, Fortune F, Stanford MR, Murray PI, O'Neill LA, Wallace GR, TIRAP Ser180Leu polymorphism is associated with Behcet's disease., Rheumatology , 50, (10), 2011, p1760-5 Journal Article, 2011 DOI

Sheedy FJ, Palsson-McDermott E, Hennessy EJ, Martin C, O'Leary JJ, Ruan Q, Johnson DS, Chen Y, O'Neill LA., Negative regulation of TLR4 via targeting of the proinflammatory tumor suppressor PDCD4 by the microRNA miR-21, Nature Immunology, 11, (2), 2010, p141-147 Journal Article, 2010 DOI

McCoy CE, Sheedy FJ, Qualls JE, Doyle SL, Quinn SR, Murray PJ, O'Neill LA, IL-10 inhibits miR-155 induction by Toll-like receptors., The Journal of Biological Chemistry, 285, (27), 2010, p20492-20498 Journal Article, 2010 DOI TARA - Full Text URL

Sheedy FJ, O'Neill LA., Adding fuel to fire: microRNAs as a new class of mediators of inflammation., Annals of the Rheumatic Diseases, 67 , (Suppl 3), 2009, piii50 - iii55 Journal Article, 2009 DOI URL

Sheedy FJ, Marinou I, O'Neill LA, Wilson AG., The Mal/TIRAP S180L and TLR4 G299D polymorphisms are not associated with susceptibility to or severity of rheumatoid arthritis., Ann Rheum Dis., 2008 Journal Article, 2008 DOI URL

Sheedy F. and O'Neill L.A., The Troll in Toll: Mal and Tram as bridges for TLR2 and TLR4 signaling, Journal of Leukocyte Biology, 82, (2), 2007, p196-203 Journal Article, 2007 DOI TARA - Full Text

Research Expertise


  • Title
    • Wellmune trains monocytes to increase innate immune memory & resistance
  • Funding Agency
    • Enterprise Ireland
  • Date From
    • 01/06/2018
  • Date To
    • 31/05/2020
  • Title
    • Targeting microRNA to drive a successful host response to Mycobacterium tuberculosis
  • Funding Agency
    • Science Foundation Ireland
  • Date From
    • 09/2014
  • Date To
    • 09/2018
  • Title
    • Measuring monocyte function as a marker of cardiovascular disease and immune activation
  • Summary
    • Atherosclerosis, a leading cause of cardiovascular disease, is triggered by recruitment of macrophages to areas of lipid deposition along vessel walls. The macrophages are recruited as precursor monocytes and differentiate locally to proinflammatory macrophages. Evidence suggests the type of monocyte recruited is affected by numerous factors. This project proposes to develop technologies to profile monocyte precursors in circulation by examining key mRNA and protein expression as biomarkers. This can be applied to approaches to monitor monocytes in disease susceptible patients.
  • Funding Agency
    • Irish Heart Foundation
  • Date From
    • 02/2017
  • Date To
    • 02/2018
  • Title
    • Metabolic control of tumor associated macrophage reprogramming by microRNA-21
  • Funding Agency
    • Irish Research Council
  • Date From
    • 01/10/2017
  • Date To
    • 30/09/2021
  • Title
    • Sensing of lipids on macrophages by CD36 alters tuberculosis immune responses
  • Funding Agency
    • Wellcome Trust/TCD
  • Date From
    • 01/06/2017
  • Date To
    • 31/05/2022
  • Title
    • IFN-gamma inhibits the maturation of anti-inflammatory microRNAs during macrophage activation
  • Funding Agency
    • Irish Research Council
  • Date From
    • 01/10/2019
  • Date To
    • 30/09/2021


Bacteriology and bacterial pathogenesis; Host, Pathogen interactions; Immune system; Immunochemistry and immunogenetics; Immunodeficiency diseases; Immunology, Immunotherapy; Infectious diseases; Innate immunology; Lipid metabolism; Metabolism and metabolic diseases; Molecular Biology; Regulatory methods of gene expression; RNA processing, stability and degradation; Tumour immunology and immunotherapy; Vaccines; Vascular Biology, Thrombosis


Awards and Honours

Irish Heart Foundation Stroke Prevention Research Bursary Award 02/2017

Senior Research Fellow, Trinity College Dublin 01/2014

Researcher of the Year, Trinity Institute of Molecular Medicine 11/2013

Highest achieving undergraduate student in first year of novel BSc Genetics, UCC 06/2004


Irish Society of Immunology 2014 – 2017

American Heart Association - ATVB Council 2010 – 2013

Trinity Biochemical Society 2005 – 2017