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Dr. David Hoey
Associate Professor, Mechanical, Manuf & Biomedical Eng

Biography

Dr. David Hoey is an Associate Professor in the School of Engineering and Principal Investigator (PI) in the Trinity Centre for Biomedical Engineering and the Advanced Materials and Bioengineering Research (AMBER) Centre at Trinity College Dublin (TCD). He is also Director of the Discipline of Biomedical Engineering at TCD and Honorary Associate Professor in the University of Limerick, Ireland. He leads a multidisciplinary research group focusing on musculoskeletal mechanobiology, mechanotransduction, and materials for regeneration in which he has pioneered research on the role of the primary cilium in loading-induced bone formation. This fundamental research underpins a more translational programme aimed at developing novel mechanotherapeutics and biomaterials that mimic the beneficial effect of biophysical stimuli to treat large bone defects and orthopaedic diseases such as osteoporosis. He has extensive experience in mechanobiology and tissue engineering and is also a PI on a major bioprinting initiative at Trinity to develop next generation implantable devices and tissues for orthopaedic applications. His laboratory has been awarded >€3.4million as Principal Investigator, including two European Research Council awards (Starting grant 2013; PoC grant 2019). He is also PI on several commercial projects developing novel biomaterials for musculoskeletal and vascular repair.

Publications and Further Research Outputs

Peer-Reviewed Publications

Man, K., Barroso, I.A., Brunet, M., Peacock, B., Federici, A.S., Hoey, D.A., Cox, S.C., Controlled Release of Epigenetically-Enhanced Extracellular Vesicles from a GelMA/Nanoclay Composite Hydrogel to Promote Bone Repair, International Journal of Molecular Sciences, 23, (2), 2022, p1-22 Journal Article, 2022 DOI

Brady, R.T., O'Brien, F.J., Hoey, D.A., The Impact of the Extracellular Matrix Environment on Sost Expression by the MLO-Y4 Osteocyte Cell Line, Bioengineering, 9, (1), 2022, p1-35 Journal Article, 2022 DOI

Luo, L., Foster, N.C., Man, K., Brunet, M., Hoey, D.A., Cox, S.C., Kimber, S.J., El Haj, A.J., Hydrostatic pressure promotes chondrogenic differentiation and microvesicle release from human embryonic and bone marrow stem cells, Biotechnology Journal, 2022, p1-11 Journal Article, 2022 URL

M. Sarmast. Sh., S.George., A.B. Dayang Radiah., D.A. Hoey., N. Abdullah., S. Kamarudin, Synthesis of bioactive glass using cellulose nano fibre template, Journal of the Mechanical Behavior of Biomedical Materials, 130, (105174), 2022 Journal Article, 2022

Man K, Brunet MY, Federici AS, Hoey DA, Cox SC., An ECM-Mimetic Hydrogel to Promote the Therapeutic Efficacy of Osteoblast-Derived Extracellular Vesicles for Bone Regeneration., Frontiers in bioengineering and biotechnology, 10, 2022, p829969 Journal Article, 2022 DOI

Man, K., Louth, S., Brunet, M., Hoey D.A., Cox, S.C., , The influence of scaffold architecture and composition on osteoblast-derived extracellular vesicles therapeutic efficacy for bone regeneration, 6th World Congress of the Tissue Engineering and Regenerative Medicine International Society, The Netherlands, 2021 Conference Paper, 2021

Whelan, I., Burdis, R., Moeendarbary, E., Hoey, D.A., Kelly, D.J., , Vascularising developing bone organoids on a chip to model endochondral ossification and fracture healing , 6th World Congress of the Tissue Engineering and Regenerative Medicine International Society, The Netherlands, 2021 Conference Paper, 2021

Federici, A., Kelly, D.J., Hoey, D.A., , Development of bio-inspired Melt-Electrowritten scaffolds for vascular regeneration, 6th World Congress of the Tissue Engineering and Regenerative Medicine International Society, The Netherlands, 2021 Conference Paper, 2021

Man K, Brunet MY, Fernandez-Rhodes M, Williams S, Heaney LM, Gethings LA, Federici A, Davies OG, Hoey D, Cox SC., Epigenetic reprogramming enhances the therapeutic efficacy of osteoblast-derived extracellular vesicles to promote human bone marrow stem cell osteogenic differentiation., Journal of extracellular vesicles, 10, (9), 2021, pe12118 Journal Article, 2021 DOI

Anushree Dwivedi, Patrick A. Kiely, David A. Hoey, Mechanically stimulated osteocytes promote the proliferation and migration of breast cancer cells via a potential CXCL1/2 mechanism, Biochemical and Biophysical Research Communications, 534, 2021, p14--20 Journal Article, 2021 TARA - Full Text

Nieuwoudt M, Woods I, Eichholz KF, Martins C, McSweeney K, Shen N, Hoey DA., Functionalization of Electrospun Polycaprolactone Scaffolds with Matrix-Binding Osteocyte-Derived Extracellular Vesicles Promotes Osteoblastic Differentiation and Mineralization., Annals of biomedical engineering, 2021, p1 - 15 Journal Article, 2021 DOI

Man, K., Brunet, M., Fernandez-Rhodes, M., Williams, S., Davies, O., Federici, A., Hoey D.A., Cox, S.C., , Epigenetic reprogramming enhances the therapeutic efficacy of osteoblast-derived extracellular vesicles for bone regeneration, 6th World Congress of the Tissue Engineering and Regenerative Medicine International Society, The Netherlands, 2021 Conference Paper, 2021

Ahern DP, McDonnell JM, Riffault M, Evans S, Wagner SC, Vaccaro AR, Hoey DA, Butler JS., A meta-analysis of the diagnostic accuracy of hounsfield units on computed topography relative to dual-energy X-ray absorptiometry for the diagnosis of osteoporosis in the spine surgery population., The spine journal : official journal of the North American Spine Society, 2021 Journal Article, 2021 DOI TARA - Full Text

Whelan IT, Moeendarbary E, Hoey DA, Kelly DJ., Biofabrication of vasculature in microphysiological models of bone., Biofabrication, 13, (3), 2021 Journal Article, 2021 TARA - Full Text DOI

Hoey. D.A., Mechano-biologically inspired vesicle-based therapeutics and materials for bone regeneration, WebEVTalk, YouTube, 2021 Invited Talk, 2021

Man K, Brunet MY, Louth S, Robinson TE, Fernandez-Rhodes M, Williams S, Federici AS, Davies OG, Hoey DA, Cox SC., Development of a Bone-Mimetic 3D Printed Ti6Al4V Scaffold to Enhance Osteoblast-Derived Extracellular Vesicles' Therapeutic Efficacy for Bone Regeneration., Frontiers in bioengineering and biotechnology, 9, 2021, p757220 Journal Article, 2021 DOI

Brunet, M.Y., Man, K., Williams, S., Davies, O., Hoey D.A., Jones, M-C., Cox, S.C., , Isolation and characterisation of osteoblast-derived extracellular vesicles for the development of bio-inspired osteogenic proteoliposomes., TRAIN-EV International Conference, Dublin, Ireland, 2021 Conference Paper, 2021

Kian F. Eichholz, Angelica Federici, Mathieu Riffault, Ian Woods, Olwyn R. Mahon, Lorraine O"Driscoll, David A. Hoey, Extracellular Vesicle Functionalized Melt Electrowritten Scaffolds for Bone Tissue Engineering, Advanced NanoBiomed Research, 2021, p2100037 Journal Article, 2021 DOI

Andromachi Pouikli, Swati Parekh, Monika Maleszewska, Chrysa Nikopoulou, Maarouf Baghdadi, Ignacio Tripodi, Kat Folz-Donahue, Yvonne Hinze, Andrea Mesaros, David Hoey, Patrick Giavalisco, Robin Dowell, Linda Partridge, Peter Tessarz, Chromatin remodeling due to degradation of citrate carrier impairs osteogenesis of aged mesenchymal stem cells, Nature Aging, 2021 Journal Article, 2021 DOI

Man, K., Louth, S., Brunet, M., Hoey D.A., Cox, S.C., , The influence of 3D printed scaffold architecture on osteoblast-derived extracellular vesicles therapeutic efficacy for bone repair, International Society of Extracellular Vesicles, Virtual, 2021 Conference Paper, 2021

Riffault, M., Johnson, G.P., Javaheri, B., Pitsillides, A., Hoey D.A., , Loss of IFT88/primary cilia in leptin receptor expressing stromal cells selectively attenuates loading-induced endosteal bone formation, Orthopaedic Research Society, Virtual, 2021 Conference Paper, 2021

Geoghegan IP, McNamara LM, Hoey DA., Estrogen withdrawal alters cytoskeletal and primary ciliary dynamics resulting in increased Hedgehog and osteoclastogenic paracrine signalling in osteocytes., Scientific reports, 11, (1), 2021, p9272 Journal Article, 2021 DOI TARA - Full Text

Man, K., Brunet, M., Fernandez-Rhodes, M., Williams, S., Davies, O., Federici, A., Hoey D.A., Cox, S.C., , Epigenetic reprogramming enhances the therapeutic efficacy of osteoblast-derived extracellular vesicles for bone regeneration, International Society of Extracellular Vesicles, Virtual, 2021 Conference Paper, 2021

Johnson GP, Fair S, Hoey DA, Primary cilium-mediated MSC mechanotransduction is dependent on Gpr161 regulation of hedgehog signalling., Bone, 2021 Journal Article, 2021 TARA - Full Text

Hoey. D.A., Mechano-biologically inspired vesicle-based therapeutics and materials for bone regeneration, Beacon Mini Symposium, Virtual, 2021, Ireland-Canada University Foundation Invited Talk, 2021

Eichholz, K., Woods, I., Riffault, M., Johnson, G.P., Shen, N., Corrigan, M., Labour, M.N., Wynne, K., Lowery, M., O'Driscoll, L., Hoey D.A., , Therapeutic delivery of mechanically activated osteocyte-derived extracellular vesicles (MAEVs) for bone regeneration, Orthopaedic Research Society, Arizona, US, 2020 Conference Paper, 2020

Mathieu Riffault, Gillian P Johnson, Madeline M Owen, Behzad Javaheri, Andrew A Pitsillides, David A Hoey, Loss of Adenylyl Cyclase 6 in Leptin Receptor"Expressing Stromal Cells Attenuates Loading"Induced Endosteal Bone Formation, JBMR Plus, 2020 Journal Article, 2020 TARA - Full Text

Khatib, N., Hoey, D.A., Nowlan, N., , Which cell mechanosensors are most critical to normal development of cartilage and ossification in the developing mouse hindlimb , EMBO Workshop - Limb Development: New Tools for a Classic Model System, Barcelona, Spain, 2020 Conference Paper, 2020

Riffault, M., Johnson, G.P., Owen, M., Javaheri, B., Pitsillides, A., Hoey D.A., , Leptin Receptor expressing stromal cells give rise to a bone lining cell population that mediates loading induced bone formation via adenylyl cyclase 6., Orthopaedic Research Society, Arizona, US. , 2020 Conference Paper, 2020

Kian F. Eichholz and Stanislas Von Euw and Ross Burdis and Daniel J. Kelly and David A. Hoey, Development of a New Bone"Mimetic Surface Treatment Platform: Nanoneedle Hydroxyapatite (nnHA) Coating, Advanced Healthcare Materials, 2020, p2001102 Journal Article, 2020 TARA - Full Text

Parmentier L, Riffault M, Hoey DA., Utilizing Osteocyte Derived Factors to Enhance Cell Viability and Osteogenic Matrix Deposition within IPN Hydrogels., Materials (Basel, Switzerland), 13, (7), 2020 Journal Article, 2020 TARA - Full Text DOI

Hoey. D.A., Mechanotherapeutics - Targeting Mechanobiological Cues within the Stem Cell Niche to drive Osteogenesis and Bone Regeneration, School of Pharmacy Seminar Series, Virtual, 2020, School of Pharmacy, University of Dublin, Trinity College Invited Talk, 2020

Eichholz KF, Woods I, Riffault M, Johnson GP, Corrigan M, Lowry MC, Shen N, Labour M, Wynne K, O'Driscoll L, Hoey DA., Human bone marrow stem/stromal cell osteogenesis is regulated via mechanically activated osteocyte-derived extracellular vesicles, Stem Cells Translational Medicine , 2020 Journal Article, 2020 TARA - Full Text DOI

Hoey. D.A., Melt Electrospun Written Scaffolds with Optimised Fibrous and Mineral Architectures to enhance human MSC Osteogenesis for Bone Regeneration, Tissue Engineering and Regenerative Medicine International Society EU Meeting, Rhodes, Greece, 2019, Tissue Engineering and Regenerative Medicine International Society Invited Talk, 2019

Corrigan MA, Coyle S, Eichholz KF, Riffault M, Lenehan B, Hoey DA., Aged Osteoporotic Bone Marrow Stromal Cells Demonstrate Defective Recruitment, Mechanosensitivity, and Matrix Deposition., Cells, tissues, organs, 207, (2), 2019, p83-96 Journal Article, 2019 DOI

Geoghegan IP, Hoey DA, McNamara LM., Integrins in Osteocyte Biology and Mechanotransduction., Current osteoporosis reports, 17, (4), 2019, p195-206 Journal Article, 2019 DOI

Primary Cilia Mechanobiology in, editor(s)Neibur, G , Mechanobiology, Elsevier, 2019, [Ahern D.A., McFie, M.R., Thompson, C.L., Duffy, M.P., Butler, J.S., Hoey, D.A., ] Book Chapter, 2019

Hoey, D.A., Targeting Mechanobiological Cues within the Stem Cell Niche to drive Osteogenesis and Bone Regeneration, 16th International Symposium on Computer Methods in Biomechanics and Biomedical Engineering, New York, US, 2019, 16th International Symposium on Computer Methods in Biomechanics and Biomedical Engineering Invited Talk, 2019

Brennan CM, Eichholz KF, Hoey DA., The effect of pore size within fibrous scaffolds fabricated using melt electrowriting on human bone marrow stem cell osteogenesis., Biomedical materials (Bristol, England), 14, (6), 2019, p065016 Journal Article, 2019 DOI TARA - Full Text

Whelan, I.T., Moeendarbary, E., Hoey D.A., Kelly, D.J., , A Microphysiological Model of Vasculature and MSC-derived tissue interaction, Organ on a Chip Meeting, The Netherlands, 2019 Conference Paper, 2019

Geoghegan IP, Hoey DA, McNamara LM., Estrogen deficiency impairs integrin "vß3-mediated mechanosensation by osteocytes and alters osteoclastogenic paracrine signalling., Scientific reports, 9, (1), 2019, p4654 Journal Article, 2019 TARA - Full Text DOI

Cilia Mediated Signalling in, editor(s)Mone Zaidi , Encyclopaedia of Bone Biology, Elsevier, 2019, [Johnson, G.J., Riffault, M., Woods, I., Hoey, D.A., ] Book Chapter, 2019

Hoey. D.A., Targeting Mechanobiological Cues within the Stem Cell Niche to drive Osteogenesis and Bone Regeneration, Healthcare Technologies Institute Seminar Series, University of Birmingham, Birmingham, UK, 2019, University of Birmingham, Birmingham, UK Invited Talk, 2019

M. A. Corrigan, T. M. Ferradaes, M. Riffault, D. A. Hoey, Ciliotherapy Treatments to Enhance Biochemically- and Biophysically-Induced Mesenchymal Stem Cell Osteogenesis: A Comparison Study, Cellular and Molecular Bioengineering, 12, (1), 2019, p53--67 Journal Article, 2019 TARA - Full Text

Stavenschi E, Hoey DA., Pressure-induced mesenchymal stem cell osteogenesis is dependent on intermediate filament remodeling., FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33, (3), 2019, p4178-4187 Journal Article, 2019 DOI TARA - Full Text

Greaney, C., Duffy, C., Hoey D.A., Monaghan, M.G., , Discerning the effect if substrate sirected differentiation on mesenchymal stromal cell metabolic activity using FLIM, European Orthopaedic Research Society, Galway, Ireland, 2018 Conference Paper, 2018

Brennan C., Eichholz, K., Hoey, D.A., , Melt Electrospun Written Constructs for Skeletal Tissue Regeneration: The Effect of Pore Size. , Orthopaedic Research Society, New Orleans, US, 2018 Conference Paper, 2018

Johnson, G.P., Stavenschi, E., Eichholz, K., Corrigan, M., Hoey D.A., , The primary cilium as a potential cAMP responsive mechanosensory in mesenchymal stem cells, World Congress in Biomechanics, Dublin, Ireland, 2018 Conference Paper, 2018

Eichholz, K., Johnson, G.P., Corrigan, M., Labour, M.N., Wynne, K., Hoey D.A., , Quantitative analysis of the osteocyte secretome following oscillatory fluid shear stimulation , Orthopaedic Research Society, New Orleans, US, 2018 Conference Paper, 2018

Eichholz, K., Hoey D.A., , Melt electrospun written constructs for skeletal tissue regeneration: The effect of fibre architecture on YAP signalling and hMSC differentiation , World Congress in Biomechanics, Dublin, Ireland, 2018 Conference Paper, 2018

Michele A. Corrigan, Gillian P. Johnson, Elena Stavenschi, Mathieu Riffault, Marie-Noelle Labour, David A. Hoey, TRPV4-mediates oscillatory fluid shear mechanotransduction in mesenchymal stem cells in part via the primary cilium, Scientific Reports, 8, (1), 2018 Journal Article, 2018 TARA - Full Text

Johnson, G.P., Stavenschi, E., Ecihholz, K., Corrigan, M., Hoey D.A., , The primary cilium as a potential cAMP responsive mechanosensory in mesenchymal stem cells. , European Orthopaedic Research Society, Galway, Ireland, 2018 Conference Paper, 2018

Gillian P. Johnson, Elena Stavenschi, Kian F. Eichholz, Michele A. Corrigan, Sean Fair, David A. Hoey, Mesenchymal stem cell mechanotransduction is cAMP dependent and regulated by adenylyl cyclase 6 and the primary cilium, Journal of Cell Science, 2018, pjcs.222737 Journal Article, 2018 TARA - Full Text

Geoghegan, I.P., Hoey, D.A., McNamara, L., , The role of integrin αvβ3 in osteocyte mechanotransduction during estrogen deficiency, World Congress in Biomechanics, Dublin, Ireland, 2018 Conference Paper, 2018

Hoey, D.A., , Loading-Induced Bone Formation: A Role for the Skeletal Stem Cell Primary Cilium, European Orthopaedic Research Society, Galway, Ireland, 2018, European Orthopaedic Research Society Invited Talk, 2018

Hoey. D.A., Skeletal Stem Cell Contributions to the Mechanoadaptation of Bone: Direct and Indirect Biophysical Regulation, Gordon Research Conference in Musculoskeletal Biology and Bioengineering, Proctor Academy, US, 2018, Gordon Research Conference Invited Talk, 2018

Marie-Noelle Labour, Marina Walsh, Marie Cavaignac, Kian Eichholz, Eamonn deBarra, David A. Hoey, Electrospun Poly-D-L-lactic acid fibrous scaffolds as a delivery vehicle for calcium phosphate salts to promote in situ mineralisation and bone regeneration, Journal of Biomaterials and Tissue Enginering, 8, 2018, p1 - 12 Journal Article, 2018 TARA - Full Text

Corrigan, M., Ferradaes, T., Hoey, D.A., , Can modulating the primary cilium influence bone formation: A comparison of ciliotherapy treatments on stem cell osteogenesis, Orthopaedic Research Society, New Orleans, US, 2018 Conference Paper, 2018

Dwivedi, A., McCumiskey, M., Hoey, D.A., Kiely, P.A., , Deciphering factors involved in breast cancer etastasis to the one, Irish Association for Cancer Research , Ireland, 2018 Conference Paper, 2018

Corrigan, M., Labour, M.N., Riffault, M., Hoey, D.A.,, TRPV4-mediates oscillatory fluid shear mechanotransduction in Mesenchymal Stem Cells in part via the primary cilium, World Congress in Biomechanics, Dublin, Ireland, 2018 Conference Paper, 2018

Riffault, M., Johnson, G., Javaheri, B., Pitsillides, A., Hoey D.A., , Characterisation of Leptin-Receptor as a marker to study skeletal stem cell contributions to bone mechanoadaptation in vivo, Gordon Research Conference, Musculoskeletal Biology and Bioengineering, US, 2018 Conference Paper, 2018

Woods, I., Shen, N., Eichholz, K., Hoey D.A., , Mechanically activated osteocyte extracellular vesicles promote osteogenic differentiation of human skeletal stem cells, World Congress in Biomechanics, Dublin, Ireland, 2018 Conference Paper, 2018

Riffault, M., Hoey, D.A., , Role of ATP signalling in primary cilium mediated mechanotransduction in human skeletal stem cells, Orthopaedic Research Society, New Orleans, US, 2018 Conference Paper, 2018

Whelan, I.T., Hoey D.A., Kelly, D.J., , Development of a vascularised bone on a chip , Organ on Chip Meeting, The Netherlands, 2018 Conference Paper, 2018

Eichholz KF, Hoey DA, Mediating human stem cell behaviour via defined fibrous architectures by melt electrospinning writing., Acta biomaterialia, 2018 Journal Article, 2018 TARA - Full Text DOI

Geoghegan, I.P., Hoey, D.A., McNamara, L., , The role of integrin αvβ3 in osteocyte mechanotransduction during estrogen deficiency, Orthopaedic Research Society, New Orleans, US, 2018 Conference Paper, 2018

Hoey, D.A., Stem Cell Contributions to Bone Mechanoadaptation: Direct and Indirect Biophysical Regulation, World Congress in Biomechanics, Dublin, Ireland, 2018, World Congress in Biomechanics Invited Talk, 2018

Johnson, G.P., Stavenschi, E., Eichholz, K., Corrigan, M., Hoey D.A., The primary cilium as a potential cAMP responsive mechanosensory in mesenchymal stem cells. , Orthopaedic Research Society, New Orleans, US, 2018 Conference Paper, 2018

Riffault, M., Hoey, D.A., , Role of ATP signalling in primary cilium mediated mechanotransduction in human skeletal stem cells., World Congress in Biomechanics, Dublin, Ireland, 2018 Conference Paper, 2018

Kelly NP, Flood HD, Hoey DA, Kiely PA, Giri SK, Coffey JC, Walsh MT, Direct mechanical characterization of prostate tissue-a systematic review., The Prostate, 2018 Journal Article, 2018 TARA - Full Text

Hoey D.A., , Loading-Induced Bone Formation: A Role for the Skeletal Stem Cell Primary Cilium, European Orthopaedic Research Society, Galway, Ireland, 2018 Conference Paper, 2018

Hoey, D.A., , Stem Cell Contributions to the Mechanoadaptation of Bone, ARI-TCBE-TERG Research Symposium, Dublin, Ireland, 2018, ARI-TCBE-TERG Research Symposium Invited Talk, 2018

Agarwal S, Labour MN, Hoey D, Duffy B, Curtin J, Jaiswal S, Enhanced corrosion resistance and cytocompatibility of biomimetic hyaluronic acid functionalised silane coating on AZ31 Mg alloy for orthopaedic applications., Journal of materials science. Materials in medicine, 2018 Journal Article, 2018 TARA - Full Text

Elena Stavenschi, Michele A. Corrigan, Gillian P. Johnson, Mathieu Riffault, David A. Hoey, Physiological cyclic hydrostatic pressure induces osteogenic lineage commitment of human bone marrow stem cells: a systematic study, Stem Cell Research & Therapy, 9, (1), 2018 Journal Article, 2018

Sankalp Agarwal, Mathieu Riffault, David Hoey, Brendan Duffy, James Curtin, Swarna Jaiswal, Biomimetic Hyaluronic Acid-Lysozyme Composite Coating on AZ31 Mg Alloy with Combined Antibacterial and Osteoinductive Activities, ACS Biomaterials Science & Engineering, 3, (12), 2017, p3244--3253 Journal Article, 2017 TARA - Full Text

Stavenschi, E., Labour, M.-N., Hoey, D.A., Oscillatory fluid flow induces the osteogenic lineage commitment of mesenchymal stem cells: The effect of shear stress magnitude, frequency, and duration, Journal of Biomechanics, 55, 2017, p99-106 Journal Article, 2017

Stavenschi, E., Labour, M.N., Hoey, D.A., , Oscillatory Fluid Flow Induces MSC Osteogenic Lineage Commitment In A Magnitude And Frequency Dependent Manner. , Orthopaedic Research Society, US, 2017 Conference Paper, 2017

Hoey, D.A., Primary Cilium-Mediated Stem Cell Mechanotransduction and Recruitment in Bone, Orthopaedic Research Society Ambassador Symposium, Dublin, Ireland, 2017, Orthopaedic Research Society Ambassador Symposium Invited Talk, 2017

Johnson, G.P., English, A.-M., Cronin, S., Hoey, D.A., Meade, K.G., Fair, S., Genomic identification, expression profiling, and functional characterization of CatSper channels in the bovine, Biology of Reproduction, 97, (2), 2017, p302-312 Journal Article, 2017 TARA - Full Text

Geoghegan, I.P., Hoey, D.A., McNamara, L., , The role of integrin αvβ3 in osteocyte mechanotransduction during estrogen deficiency., Summer Biomechanics, Bioengineering and Biotransport Conference (SB3C2017),, US, 2017 Conference Paper, 2017

Geoghegan, I.P., Hoey, D.A., McNamara, L., , The role of integrin αvβ3 in osteocyte mechanotransduction during estrogen deficiency, Matrix Biology Ireland, Dublin, Ireland, 2017 Conference Paper, 2017

Corrigan, M., Labour, M.N., Riffault, M., Hoey, D.A., , Fluid Shear Induces TRPV4 Mediated Calcium Influx Contributing To Osteogenic Responses In MSCs,, Orthopaedic Research Society, US, 2017 Conference Paper, 2017

Eichholz, K., Hoey, D.A., , Melt Electrospun Written Scaffolds as in-vitro platforms for stem cell culture: The effect of scaffold architecture. , TERMIS-EU, Davos, Switzerland, 2017 Conference Paper, 2017

Stavenschi, E., Hoey, D.A., , Cyclic hydrostatic pressure mediates early osteogenesis via cytoskeletal remodelling in mesenchymal stem cells. , European Society of Biomechanics, Spain, 2017 Conference Paper, 2017

Flanagan AM, Stavenschi E, Basavaraju S, Gaboriau D, Hoey DA, Morrison CG, Centriole splitting caused by loss of the centrosomal linker protein C-NAP1 reduces centriolar satellite density and impedes centrosome amplification., Molecular Biology of the Cell, 28, (6), 2017, p736-745 Journal Article, 2017

Corrigan, M., Labour, M.N., Riffault, M., Hoey, D.A., , TRPV4-mediates oscillatory fluid shear mechanotransduction in Mesenchymal Stem Cells in part via the primary cilium, Matrix Biology Ireland, Dublin, Ireland, 2017 Conference Paper, 2017

The Role of the Primary Cilium in Cellular Mechanotransduction: An Emerging Therapeutic Target in, editor(s)Rawlinson, S. , Mechanobiology: Exploitation for Benefit, Wiley, 2016, pp61-73 , [Eichholz, K.F., Hoey, D.A.] Book Chapter, 2016

Labour, M.N., Coyle, S., Lenehan, B., Christensen, S.T., Hoey, D.A., , TGFβ-1induced recruitment of human mesenchymal stem cells is mediated by the primary cilium in a SMAD3-dependent manner, Musculoskeletal Biology & Bioengineering Gordon Research Conference, US, 2016 Conference Paper, 2016

Electromechanics of bone: Mechanobiological considerations in, editor(s)Tofail, S , Electrically Active Materials for Medical Devices, World Scientific, 2016, pp511-528 , [Coyle, S., Lenehan, B., Hoey, D.A.] Book Chapter, 2016

Sankalp Agarwal, Muhammad Morshed, Marie-Noelle Labour, David Hoey, Brendan Duffy, James Curtin, Swarna Jaiswal, Enhanced corrosion protection and biocompatibility of a PLGA-silane coating on AZ31 Mg alloy for orthopaedic applications, RSC advances, 6, (115), 2016, p113871-113883 Journal Article, 2016 DOI

Johnson, G., Lyons, A., Cronin, S., Hoey, D.A., Fair, S., , CatSper channels regulate bull sperm hyperactivation and rheotaxic response, International Congress on Animal Reproduction, France, 2016 Conference Paper, 2016

Geoghegan, I.P., Hoey, D.A., McNamara, L., , The role of integrin αvβ3 in osteocyte mechanotransduction during estrogen deficiency, Matrix Biology Ireland, Galway, Ireland, 2016 Conference Paper, 2016

Johnson, G., English. A.M., Hoey, D.A., Meade, K.G., Fair, S., , Comparative Genomic Identification and Expression Profiling of CatSper Genes in the Reproductive Tract of the Bull, The Association for Animal Andrology, France, 2016 Conference Paper, 2016

Labour MN, Riffault M, Christensen ST, Hoey DA, TGFß1 - induced recruitment of human bone mesenchymal stem cells is mediated by the primary cilium in a SMAD3-dependent manner., Nature Scientific Reports, 2016 Journal Article, 2016 TARA - Full Text

Labour, M.N., Coyle, S., Lenehan, B., Christensen, S.T., Hoey, D.A., , TGFβ-1induced chemotaxis in human mesenchymal stem cells requires the primary cilium and is defective in osteoporosis, Orthopaedic Research Society, US, 2016 Conference Paper, 2016

Stavenschi, E., Labour, M.N., Hoey, D.A, The Effect Of Static And Dynamic Pressure On Osteoprogenitor And Osteoblast Gene Expression And Cytoskeleton In Vitro, Orthopaedic Research Society,, US, 2016 Conference Paper, 2016

O'Connor Mooney R, Davis NF, Hoey D, Hogan L, McGloughlin TM, Walsh MT, On the Automatic Decellularisation of Porcine Aortae: A Repeatability Study Using a Non-Enzymatic Approach., Cells, Tissues, Organs, 2016 Journal Article, 2016

Hoey, D.A., Deciphering the role of the cilium in skeletal stem cell physiology, Membrane Trafficking, Cilia ad Oxygen Sensing, Tokyo, Japan, 2016, Membrane Trafficking, Cilia ad Oxygen Sensing Committee Invited Talk, 2016

Hoey, D.A., Deciphering the molecular mechanisms of stem cell mechanotransduction: A role for the primary cilium, Irish Mechanobiology Network Meeting, Dublin, Ireland, 2016, Irish Mechanobiology Network Invited Talk, 2016

Johnson, G., Corrigan, M., Hoey, D.A., , The role of Adenylyl Cyclase 6 in Mesenchymal Stem Cell Mechanotransduction, Orthopaedic Research Society, US, 2016 Conference Paper, 2016

Labour, M.N., Hoey, D.A., , TGFβ1 Signalling in Human Mesenchymal Stem Cells is regulated by the Primary Cilium, Orthopaedic Research Society, Las Vegas, US, 2015 Conference Paper, 2015

Brady, R.T., Hoey, D.A., O'Brien, F.J., , Osteocyte Sclerostin Expression Is Directed By Substrate Composition And Dimensionality In Addition To Fluid Flow, Summer Biomechanics, Bioengineering and Biotransport Conference (SB3C2015), , Utah, US, 2015 Conference Paper, 2015

Brady RT, O'Brien FJ, Hoey DA, Mechanically stimulated bone cells secrete paracrine factors that regulate osteoprogenitor recruitment, proliferation, and differentiation., Biochemical and biophysical research communications, 459, (1), 2015, p118 - 123 Journal Article, 2015 TARA - Full Text

Hoey, D.A., Primary Cilium-Mediated Mesenchymal Stem Cell Mechanobiology in Bone, Queens University Belfast ERC Workshop, Queens University Belfast, 2015, Queens University Belfast Invited Talk, 2015

Corrigan, M., Lee, K.L., Labour, M.N., Jacobs, C.R., Hoey, D.A., , Quantification of Fluorescent Calcium Biosensor Signals in Real-time within Discrete Subcellular Compartments of Mesenchymal Stem Cells under physiological loading, Microscopy Ireland, Limerick, Ireland, 2015 Conference Paper, 2015

Labour, M.N., Cavaignac, M., deBarra, E., Hoey, D.A., , Development of a Composite PolyD,L-lactic acid / Calcium Phosphate Electrospun Fibrous Scaffold for Bone Tissue Regeneration, Orthopaedic Research Society, Las Vegas, US, 2015 Conference Paper, 2015

Corrigan, M., Lee, K.L., Labour, M.N., Jacobs, C.R., Hoey, D.A., , Calcium signalling in Response to Fluid Flow within the Primary Cilium Microdomain of Mesenchymal Stem Cells, Orthopaedic Research Society, Las Vegas, US, 2015 Conference Paper, 2015

Stavenschi, E., Labour, M.N., Hoey, D.A., , A Simple In-Vitro Fluid Flow Stimulation Platform To Enhance Osteogenesis Of Human Mesenchymal Stem Cells, Orthopaedic Research Society, Las Vegas, US, 2015 Conference Paper, 2015

Hoey DA, Chen JC, Chua M, Bellon R, Jacobs CR, Mechanical signals promote osteogenic fate through a primary cilia-mediated mechanism., FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2015 Journal Article, 2015 DOI TARA - Full Text

Lee K., Hoey D., Hoey D., Hoey D., Spasic M., Spasic M., Tang T., Tang T., Hammond H., Hammond H., Jacobs C., Adenylyl cyclase 6 mediates loading-induced bone adaptation in vivo, FASEB Journal, 28, (3), 2014, p1157 - 1165 Journal Article, 2014 DOI

Downs M., Nguyen A., Herzog F., Hoey D., Hoey D., Hoey D., Jacobs C., An experimental and computational analysis of primary cilia deflection under fluid flow, Computer Methods in Biomechanics and Biomedical Engineering, 17, (1), 2014, p2 - 10 Journal Article, 2014 DOI

Browne L., O'Callaghan S., Hoey D., Griffin P., McGloughlin T., Walsh M., Correlation of Hemodynamic Parameters to Endothelial Cell Proliferation in an End to Side Anastomosis, Cardiovascular Engineering and Technology, 5, (1), 2014, p110 - 118 Journal Article, 2014 DOI

Brady, R.T., McCoy, R., Hoey, D.A., O'Brien, F.J., , Substrate Stiffness and Fluid Flow Act Synergistically to Direct Osteogenic Differentiation, World Congress of Biomechanics, Boston, US, 2014 Conference Paper, 2014

Hoey, D.A., Impact of the ERC on my Research and Career, Royal Irish Academy (ERC President Visit), Royal Irish Academy, Dublin, Ireland, 2014, Royal Irish Academy Invited Talk, 2014

Hoey, D.A., Research Strategy: Standing Out From the Crowd, University of Limerick Lunchtime Research Seminars, Limerick, Ireland, 2014, University of Limerick Invited Talk, 2014

Espinha L., Espinha L., Hoey D., Hoey D., Fernandes P., Rodrigues H., Jacobs C., Oscillatory fluid flow influences primary cilia and microtubule mechanics, Cytoskeleton, 71, (7), 2014, p435 - 445 Journal Article, 2014 DOI TARA - Full Text

Hoey, D.A., Primary Cilia Mechanobiology: from Molecule to Mouse, Irish Cilia Biology Network, Dublin, Ireland, 2014, Irish Cilia Biology Network Invited Talk, 2014

Brady, R.T., O'Brien, F.J., Hoey, D.A., , Mechanically Stimulated Osteocytes Direct Stem Cell Proliferation and Recruitment: an in Vitro Study, Orthopaedic Research Society Meeting, New Orleans, US, 2014 Conference Paper, 2014

Hoey, D.A., , Discovering Fundamental Mechanobiological Mechanisms to Develop Bioactive Materials for Regenerative Medicine, European Materials Research Society, Warsaw, Poland, 2014 Conference Paper, 2014

Hoey, D.A., Discovering Fundamental Mechanobiological Mechanisms to Develop Bioactive Materials for Regenerative Medicine, European Materials Research Society (Fall Meeting), Warsaw, 2014, European Materials Research Society Invited Talk, 2014

Chen, J.C., Hoey, D.A., Chua, M., Bellon, R., Jacobs, C.R., , Deletion of Primary Cilia in Bone Marrow Cells Attenuates Mechanically Induced Bone Formation. , Orthopaedic Research Society Meeting, New Orleans, US, 2014 Conference Paper, 2014

Hoey, D.A., Primary Cilium-Mediated Mechanobiology in Bone, Queen Mary, University of London Seminar Series, 2013, Queen Mary, University of London Invited Talk, 2013

Kelly, N., Hoey, D.A., Walsh, M.T., Flood, H., , Mechanical Characterisation of the Prostate: Initial Results, Anatomical Society Meeting, Dublin, Ireland, 2013 Conference Paper, 2013

Espinha, L., Hoey, D.A., Fernandes, P.R., Rodrigues, H.C., Jacobs, C.R., , OFF influences the Number of Microtubules at the Base of Primary Cilia, 5th National Congress of Biomechanics,, Espinho, Portugal, 2013 Conference Paper, 2013

Chen, J.C., Hoey, D.A., Jacobs, C.R., , Primary Cilia of Bone Marrow Stromal Cells Mediate Mechanically Induced Osteogenesis, American Society of Bone and Mineral Research, Baltimore, US, 2013 Conference Paper, 2013

Hoey, D.A., , Stem Cell Contributions to Loading-Induced Bone Formation: A Role of the Primary Cilium, CLASS Conway Institute Seminar Series, UCD, Dublin, Ireland, 2013, CLASS Conway Institute, University College Dublin Invited Talk, 2013

Hoey, D.A., My ERC Application: Perspectives and Insights, University of Limerick EU grant Proposals - Maximizing your Success Workshop, Limerick, Ireland, 2013, University of Limerick Invited Talk, 2013

Chen, J.C., Hoey, D.A., Chua, M.C., Bellon, R.B., Jacobs, C.R., , The Role of Primary Cilia in Bone Marrow Stromal Cells in Mechanically Induced Osteogenesis. , New York Stem Cell (NYSTEM) Meeting, New York, US, 2013 Conference Paper, 2013

Hoey, D.A., Bone Mechanobiology - A Role for Mechanics in Treatment Development, University Hospital Limerick Grand Rounds, Limerick, Ireland, 2013, University Hospital Limerick Invited Talk, 2013

Hoey, D.A., My Career in Biomaterials: A case study of challenges that led to opportunities, European Society of Biomaterials Meeting, Young Scientist Workshop "Career Development - Challenges and Opportunities", , Madrid, Spain, 2013, European Society of Biomaterials Invited Talk, 2013

Hoey, D.A., Primary Cilium-mediated Stem Cell Mechanobiology in Bone, Hospital for Special Surgery Seminar Series, New York, US, 2013, Hospital for Special Surgery Invited Talk, 2013

Espinha, L., Jacobs, C.R., Hoey, D.A., Oscillatory Fluid Flow Influences the Number of Microtubules Attached to the Base of Primary Cilia, Orthopedic Research Society, San Francisco, US, 2012 Conference Paper, 2012

Lee, K.L., Hoey, D.A., Tang, T., Hammand, K.H., Jacobs, C.R., , Adenylyl Cyclase 6 Mediates Bone Adaptation In Vivo, Orthopedic Research Society, San Francisco, US, 2012 Conference Paper, 2012

Espinha, L., Jacobs, C.R., Hoey, D.A., , Oscillatory Fluid Flow Influences the Number of Microtubules Attached to the Base of Primary Cilia, European Society of Biomechanics, Lisbon, Portugal, 2012 Conference Paper, 2012

Lee, K.L., Hoey, D.A., Spasic, M., Tang, T., Hammand, K.H., Jacobs, C.R., , Involvement of Adenylyl Cyclase 6 in Mediating Loading induced Bone Formation. , Society for Physical Regulation in Biology and Medicine Conference, Puerto Rico, 2012 Conference Paper, 2012

Hoey, D.A., Downs, M.E., Jacobs, C.R., The mechanics of the primary cilium: An intricate structure with complex function, Journal of Biomechanics, 45, (1), 2012, p17-26 Journal Article, 2012

Hoey, D.A., The Broad Spectrum of Bioengineering: Biomaterials to Mechanobiology, Materials and Surface Science Institute Research Forum, University of Limerick, Ireland, 2012, Materials and Surface Science Institute Invited Talk, 2012

Hoey, D.A., A Role for the Primary Cilium in Bone Mechanobiology, Biomechanics Seminar Series at the National University of Ireland, National University of Ireland, Galway, Ireland, 2012 Invited Talk, 2012

Hoey D., Hoey D., Hoey D., Chen J., Jacobs C., The primary cilium as a novel extracellular sensor in bone, Frontiers in Endocrinology, 3, (JUN), 2012 Journal Article, 2012 DOI

Chen, J.C., Hoey, D.A., Jacobs, C.R., The role of Bone Marrow Stem Cells in Loading Induced Osteogenesis, Society for Physical Regulation in Biology and Medicine Conference, Puerto Rico, 2012 Conference Paper, 2012

Hoey D., Hoey D., Hoey D., Tormey S., Ramcharan S., O'Brien F., O'Brien F., Jacobs C., Primary cilia-mediated mechanotransduction in human mesenchymal stem cells, Stem Cells, 30, (11), 2012, p2561-2570 Journal Article, 2012 DOI

Hoey, D.A., Tormey, S., O'Brien, F.J., Jacobs, C.R., , A Mechanosensory Role for the Primary Cilium in Human Mesenchymal Stem Cells, Orthopedic Research Society, San Francisco, US, 2012 Conference Paper, 2012

Hoey, D.A., Biomaterials and Mechanobiology: Studies in Bone, Materials Ireland Symposium, University of Limerick, 2012, Materials Ireland Invited Talk, 2012

Hoey, D.A., Tormey, S., O'Brien, F.J., Jacobs, C.R., , Fluid flow sensing via the Primary Cilium in Human Mesenchymal Stem Cells, Exciting Biologies: Forces in Biology, Dublin, Ireland, 2012 Conference Paper, 2012

Hoey, D.A., Jacobs, C.R., , Primary Cilia Mediate the Upregulation of COX2 and BMP2 in Response to Oscillatory Fluid Flow in Human Mesenchymal Stem , New York Stem Cell (NYSTEM) Meeting, New York, US, 2011 Conference Paper, 2011

Hoey, D.A., Carette, D., O'Reilly, P., Taylor, D., , The Effect of Stem Geometry in a Porous Bone Cement Mantle, Orthopaedic Research Society, Long Beach, US, 2011 Conference Paper, 2011

Hoey, D.A,, The Primary Cilium: Fact Not Fiction, The Royal College of Surgeons in Ireland, TERG Seminar Series, The Royal College of Surgeons in Ireland, Dublin, Ireland, 2011, The Royal College of Surgeons in Ireland Invited Talk, 2011

Hoey, D.A., Tormey, S., Jacobs, C.R., , A Role for the Primary Cilium in Fluid Flow Mediated Osteogenic Differentiation of Human Mesenchymal Stem Cells, 4th New York Skeletal Biology and Medicine Conference, New York, US, 2011 Conference Paper, 2011

Lee, K.L., Hoey, D.A., Tang, T., Hammand, K.H., Jacobs, C.R., , Adenylyl Cyclase 6 Mediates In Vivo Mechanotransduction In Bone, 4th International Conference on the Mechanics of Biomaterials and Tissues, Hawaii, US, 2011 Conference Paper, 2011

Hoey, D.A., Chen, J.C., Ramcharan, S., Shyer, J., Jacobs, C.R., , The effect of Oscillatory Fluid Flow on the Osteogenic Differentiation of Human Bone Marrow Stromal cells, Society for Physical Regulation in Biology and Medicine Conference, Florida, US, 2011 Conference Paper, 2011

Hoey, D.A., Kelly, D.J., Jacobs, C.R., , Fluid flow stimulation of osteocytes via the primary cilium can enhance osteogenic gene expression in MSCs by a paracrine signaling mechanism, 4th International Conference on the Mechanics of Biomaterials and Tissues, Hawaii, US, 2011 Conference Paper, 2011

Mechanobiology of Primary Cilia in, editor(s)Gefen, A , Cellular and Biomolecular Mechanics and Mechanobiology, 2011, [Kwon, R.Y., Hoey, D.A., Jacobs, C.R., ] Book Chapter, 2011

Hoey D., Jacobs C., On the horizon from the ORS, Journal of the American Academy of Orthopaedic Surgeons, 19, (1), 2011, p59-62 Journal Article, 2011

Kelly, D.J., Hoey, D.A., Jacobs, C.R., , Signals from flow stimulated osteocytes can enhance osteogenic gene expression in mesenchymal stem cells, Orthopaedic Research Society, Long Beach, US, 2011 Conference Paper, 2011

Hoey, D.A., Kelly, D.J., Jacobs, C.R., A role for the primary cilium in paracrine signaling between mechanically stimulated osteocytes and mesenchymal stem cells. , Biochemical and Biophysical Research Communications, 412, (1), 2011, p182-187 Journal Article, 2011 TARA - Full Text

Hoey, D.A., O'Brien, F.J., Jacobs, C.R., , The mechanosensing role of the primary cilium in oscillatory fluid flow driven osteogenic differentiation of human bone marrow stromal cells', 4th International Conference on the Mechanics of Biomaterials and Tissues, Hawaii, US, 2011 Conference Paper, 2011

Lee, K.L., Hoey, D.A., Tang, T., Hammand, K.H., Jacobs C.R, , TRPV4 is Involved in Mechanotransduction in MC3T3 osteoblasts, 4th New York Skeletal Biology and Medicine Conference, New York, US, 2011 Conference Paper, 2011

Downs M., Hoey, D.A., Herzog, F.A., Jacobs, C.R., , Analysis of Primary Cilia Deflection Under Laminar Fluid Flow with 3D Imaging and Advanced Modeling, Biomedical Engineering Society Conference, Austin, US, 2010 Conference Paper, 2010

Hoey, D.A., Geraedts, J., Jacobs, C.R., Determining the mechanisms of primary cilia bending in response to fluid shear stress, Society for Physical Regulation in Biology and Medicine Conference, Arizona, US, 2010 Conference Paper, 2010

Hoey, D.A., Stress Concentrations in Bone Cement: A Critical Distance Approach, Biomedical Engineering Graduate Seminar Series, Columbia University in the City of New York, New York, US, 2010, Columbia University in the City of New York Invited Talk, 2010

Hoey D and Taylor D, The effect of mixing technique on fatigue of bone cement when stress concentrations are present., International Journal of Nano and Biomaterials, 3, 2010, p36 - 48 Journal Article, 2010 DOI

Herzog, F.A., Geraedts, J., Hoey, D., Jacobs, C.R., A mathematical approach to study the bending behavior of the primary cilium, Proceedings of the North East Bioengineering Conference, New York, US, 2010 Conference Paper, 2010

Lee, K.L., Hoey, D.A., Jacobs, C.R., Primary cilia-mediated mechanotransduction in bone, Clinical Reviews in Bone and Mineral Metabolism, 8, (4), 2010, p201-212 Journal Article, 2010

Herzog, F.A., Geraedts, J., Hoey, D.A., Jacobs, C.R., , A Mathematical Approach to Study the Bending Behavior of the Primary Cilium, North East Bioengineering Conference, New York, US, 2010 Conference Paper, 2010

Hoey D and Taylor D, Comparison of the fatigue behaviour of two different forms of PMMA, Fatigue and Fracture of Engineering Materials and Structures, 32, 2009, p261 - 269 Journal Article, 2009 DOI

, The role of stress concentrations in the bone cement mantle, edited by df , (PART A), 2009, pp463-464 Conference Paper, 2009

Hoey D and Taylor D, Statistical distribution of the fatigue strength of porous bone cement. , Biomaterials, 30, 2009, p6309 - 6317 Journal Article, 2009 DOI

Taylor D and Hoey D, High cycle fatigue of welded joints: the TCD experience, International Journal of Fatigue, 31, 2009, p20 - 27 Journal Article, 2009 DOI

Hoey D and Taylor D, Quantitative analysis of the effect of porosity on the fatigue strength of bone cement., Acta Biomaterialia, 5, 2009, p719 - 726 Journal Article, 2009 DOI

Hoey D and Taylor D , Fatigue in porous PMMA: the effect of stress concentrations, International Journal of Fatigue, 30, 2008, p989 - 995 Journal Article, 2008

Hoey, D.A., , The role of stress concentrations in the fatigue strength of porous PMMA, Proceedings of 10th annual Sir Bernard Crossland Symposium, Galway, Ireland, 2007 Conference Paper, 2007

Hoey, D.A., Taylor, D., , The role of porosity in the fatigue strength of bone cement: the importance of pore size and pore distribution, Proceedings of the Second International Conference on the Mechanics of Biomaterials and Tissues, Hawaii, US, 2007 Conference Paper, 2007

Hoey, D.A., Taylor, D., , Using the Theory of Critical Distances with FEA to predict Fatigue Failure in Bone Cement, Proceedings of the European Society of Biomechanics, Dublin, Ireland, 2007, 2007 Conference Paper, 2007

Taylor, D., Hoey, D., Sanz, L., O'Reilly, P., Fracture and fatigue of bone and bone cement: The critical distance approach, 2006, pp1025-1026 Conference Paper, 2006

152. Taylor, D., Hoey, D.A., Sanz, L., O'Reilly, P.,, Fracture and fatigue of bone and bone cement: The critical distance approach., Proceedings of European Conference of Fracture, Alexandroupolis , 2006, 2006 Conference Paper, 2006

Non-Peer-Reviewed Publications

Federici, A., Eichholz, K., Cunniffe, G.M., Kelly, D.J., Hoey, D.A., , Development of bio-inspired scaffolds for vascular tissue engineering applications, 26th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2020 Conference Paper, 2020

Whelan, I., Moeendarbary, E., Hoey, D.A., Kelly, D.J., , A microphysiological model of human bone tissue , 26th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2020 Conference Paper, 2020

Riffault, M., Johnson, G.P., Owen, M., Javaheri, B., Pitsillides, A., Hoey D.A., , Leptin Receptor expressing stromal cells give rise to a bone lining cell population that mediates loading induced bone formation via adenylyl cyclase 6. , 26th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2020 Conference Paper, 2020

Geoghegan, I.P., Hoey, D.A., McNamara, L., , The role of integrin αvβ3 in osteocyte mechanotransduction during estrogen deficiency, 25th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2019 Conference Paper, 2019

Johnson, G., Corrigan, M., Stavenschi, E., Hoey, D.A., , MSC contributions to loading induced bone formation: a role for Adenylyl Cyclase 6. , 25th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2019 Conference Paper, 2019

Eichholz, K., Woods, I., Johnson, G.P., Mahon, O.R., Federici, A., Corrigan, M., Lowery, M., O'Driscoll, L., Hoey D.A., , Mimicking cellular and ECM mechanobiological cues to drive MSC osteogenesis and bone regeneration, 25th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2019 Conference Paper, 2019

Parmentier, L., Hoey D.A., , Upscaling the production of mechanically activated secreted factors and optimising their local delivery via material functionalisation, 25th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2019 Conference Paper, 2019

Federici, A., Eichholz, K., Kelly, D.J., Hoey, D.A., Development of melt electrospun written tubular scaffolds for vascular tissue engineering applications , 25th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2019 Conference Paper, 2019

Riffault, M., Hoey, D.A., , Role of ATP signalling in primary cilium mediated mechanotransduction in human skeletal stem cells. , 24th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2018 Conference Paper, 2018

Corrigan, M., Ferradaes, T., Hoey, D.A., , Can modulating the primary cilium influence bone formation: A comparison of ciliotherapy treatments on stem cell osteogenesis, 24th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2018 Conference Paper, 2018

Shen, N., Woods, I., Hoey D.A., , Mechanically activated osteoclast and osteocyte derived paracrine factors induce angiogenesis stimulation , 24th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2018 Conference Paper, 2018

Eichholz, K., Hoey, D.A., , Melt Electrospun Written Constructs for Skeletal Tissue Regeneration: The Effect of Fibre Architecture., 24th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2018 Conference Paper, 2018

Stavenschi, E., Hoey, D.A., , Cyclic hydrostatic pressure induces human SSC lineage commitment in a magnitude dependent manner. , Orthopaedic Research Society, New Orleans, US, 2018 Conference Paper, 2018

Johnson, G., Corrigan, M., Stavenschi, E., Hoey, D.A., The , The primary cilium as a pontential cAMP responsive mechanosensory in mesenchymal stem cells, 24th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2018 Conference Paper, 2018

Geoghegan, I.P., Hoey, D.A., McNamara, L., , The role of integrin αvβ3 in osteocyte mechanotransduction during estrogen deficiency. , 24th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2018 Conference Paper, 2018

Dwivedi, A., McCumiskey, M., Hoey, D.A., Kiely, P.A., , Deciphering factors involved in breast cancer metastasis to the one, European Association of Cancer Research, Ireland, 2018 Conference Paper, 2018

Whelan, I.T., Hoey D.A., Kelly, D.J., , Development of a vascularised bone on a chip , 24th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2018 Conference Paper, 2018

Woods, I., Shen, N., Eichholz, K., Hoey D.A., , Mechanically stimulated osteocytes coordinate stem cell recruitment via extracellular vesicle cell-cell communication, 24th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2018 Conference Paper, 2018

Eichholz, K., Woods, I., Shen, N., Johnson, G.P., Corrigan, M., Labour, M.N., Wynne, K., Lowery, M., O'Driscoll, L., Hoey D.A., , Quantitative analysis of the osteocyte secretome following oscillatory fluid shear stimulation., European Orthopaedic Research Society, Galway, Ireland, 2018 Conference Paper, 2018

Stavenschi, E., Hoey, D.A., , Cyclic hydrostatic pressure remodels the intermediate filament network and enhances osteogenic gene expression in mesenchymal stem cells, 24th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2018 Conference Paper, 2018

Stavenschi, E., Hoey, D.A., , Cyclic hydrostatic pressure remodels the intermediate filament network and enhances osteogenic gene expression in mesenchymal stem cells, Orthopaedic Research Society, New Orleans, US, 2018 Conference Paper, 2018

Geoghegan, I.P., Hoey, D.A., McNamara, L., , The role of integrin αvβ3 in osteocyte mechanotransduction during estrogen deficiency, European Orthopaedic Research Society, Galway, Ireland, 2018 Conference Paper, 2018

McKee, B., Corrigan, M., Hoey, D.A., , Are Mesenchymal Stem Cells more mechano-sensitive in low oxygen environments such as exists within bone marrow, 23rd Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2017 Conference Paper, 2017

Eichholz, K., Hoey, D.A., , Melt Electrospun Written Scaffolds as in-vitro platforms for stem cell culture: The effect of scaffold architecture on MSC osteogenesis. , 23rd Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland,, 2017 Conference Paper, 2017

Corrigan, M., Riffault, M., Johnson, G., Hoey, D.A., , Targeting of TRPV4 mimics the mechanotransduction response to fluid shear in Mesenchymal Stem Cells. , 23rd Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2017 Conference Paper, 2017

Brennan, C., Eichholz, K., Hoey, D.A., , The effect of pore size melt electrospun written PCL scaffolds on mesenchymal stem cell osteogenic differentiation, 23rd Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2017 Conference Paper, 2017

Geoghegan, I.P., Hoey, D.A., McNamara, L., , The role of integrin αvβ3 in osteocyte mechanotransduction during estrogen deficiency., 23rd Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2017 Conference Paper, 2017

Johnson, G., Corrigan, M., Stavenschi, E., Hoey, D.A., , Primary Cilia mediated MSC mechanotransduction is dependent on adenylyl cyclase 6 and cyclic AMP signalling, 23rd Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2017 Conference Paper, 2017

Geoghegan, I.P., Hoey, D.A., McNamara, L., , The role of integrin αvβ3 in osteocyte mechanotransduction during estrogen deficiency, UL-NUIG Research Day, Galway, 2017 Conference Paper, 2017

Stavenschi E., Hoey, D.A., , Cyclic Hydrostatic Pressure Enhances Osteogenic Gene Expression and Alters the Cytoskeletal Network of Mesenchymal Stem Cells. , 23rd Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2017 Conference Paper, 2017

Riffault, M., Hoey, D.A., , Role of ATP signalling in Fluid Flow-induced osteogenic commitment of mesenchymal stem cells, 23rd Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Ireland, 2017 Conference Paper, 2017

Eichholz, K., Hoey, D.A., , Design and Fabrication of Tissue Engineering Scaffolds using Melt Electrospinning Writing, 22nd Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Galway, Ireland, 2016 Conference Paper, 2016

Corrigan, M., Johnson, G.P., Stavenschi, E., Hoey, D.A., , TRPV4 is required for flow mediated calcium signalling and osteogenic lineage commitment Mesenchymal Stem Cells. , 22nd Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Galway, Ireland, 2016 Conference Paper, 2016

Stavenschi, E., Hoey, D.A., , The Effect Of Static And Dynamic Pressure On Early Osteogenesis in Osteoprogenitors And Osteoblasts In Vitro, 22nd Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Galway, Ireland, 2016 Conference Paper, 2016

Labour, M.N., Coyle, S., Lenehan, B., Christensen, S.T., Hoey, D.A., , TGFβ-1induced chemotaxis in human mesenchymal stem cells requires the primary cilium and is defective in osteoporosis, 22nd Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Galway, Ireland, 2016 Conference Paper, 2016

Eichholz, K., MacGuinness, A., Hoey, D.A., , Design and Fabrication of Tissue Engineering Scaffolds using Melt Electrospinning Writing., Orthopaedic Research Society, US, 2016 Conference Paper, 2016

Johnson, G., Corrigan, M., Hoey, D.A., , The role of Adenylyl Cyclase 6 in Mesenchymal Stem Cell Mechanotransduction, 22nd Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Galway, Ireland, 2016 Conference Paper, 2016

Kelly, N., Mulvihill, J., Hoey, D.A., Giri, S., Flood, H., Walsh, M.T., , Mechanical, histological and biochemical analysis of benign hyperplastic prostate tissue, 21st Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Dublin, Ireland, 2015 Conference Paper, 2015

Stavenschi, E., Labour, M.N., Hoey, D.A., , Enhanced Osteogenic Differentiation Of Human Mesenchymal Stem Cells Using A Simple In-Vitro Fluid Flow Bioreactor , 21st Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Dublin, Ireland, 2015 Conference Paper, 2015

Corrigan, M., Lee, K.L., Labour, M.N., Jacobs, C.R., Hoey, D.A., , Fluid Flow-Induced Bending of the Primary Cilium Triggers a Distinct Intraciliary Calcium Flux in Mesenchymal Stem Cells, 21st Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Dublin, Ireland, 2015 Conference Paper, 2015

Labour, M.N., Christensen, S.T., Hoey, D.A., , Regulation of TGFβ1 Signalling by the Primary Cilium in Mesenchymal Stem Cells, 21st Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Dublin, Ireland, 2015 Conference Paper, 2015

Labour, M.N., Cavaignac, M., deBarra, E., Hoey, D.A., , The development of a Self-mineralizing PolyD,L-lactic acid/Calcium Phosphate electrospun scaffold for bone tissue regeneration , 21st Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Dublin, 2015 Conference Paper, 2015

Eichholz, K.F., Hoey, D.A., , Design of a 3-axis Direct Writing Apparatus for Controlled Deposition of Melt Electrospun Fibres., 21st Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Dublin, Ireland, 2015 Conference Paper, 2015

Johnson, G., Hoey, D.A., , Investigate the expression, localization and role of Adenylyl Cyclase 6 in mesenchymal stem cell osteogenic differentiation, 21st Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Dublin, Ireland, 2015 Conference Paper, 2015

Coyle, S., Corrigan, M., Hoey, D.A., , Isolation and characterisation of Mesenchymal Stem Cells from osteoporotic/osteoarthritic patients. , 21st Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Dublin, Ireland, 2015 Conference Paper, 2015

Eichholz, K.F., Hoey, D.A., , Optimising Scaffold Morphology via the Controlled Deposition of Melt Electrospinning Fibers, University Hospital Limerick Annual Research Symposium, Limerick, Ireland, 2014 Conference Paper, 2014

Stavenschi, E., Hoey, D.A., , Design of a 2D bioreactor for fluid flow stimulation of mesenchymal stem cells: parallel plate flow chamber, 20th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Limerick, Ireland, 2014 Conference Paper, 2014

Kelly, N., Mulvihill, J., Giri, S., Hoey, D.A., Flood, H., Walsh, M.T., , Voiding symptom severity is related to prostate tissue stiffness in Benign Prostatic Hyperplasia, 20th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Limerick, Ireland, 2014 Conference Paper, 2014

Corrigan, M., Lee, K.L., Jacobs, C.R., Hoey, D.A., , Characterisation of calcium signaling within the primary cilium of stem cells using FRET biosensor technology, University Hospital Limerick Annual Research Symposium, Limerick, Ireland, 2014 Conference Paper, 2014

Labour, M.N., Hoey, D.A., , TGFβ-1 induced Stem Cell Migration in Bone is Associated with the Primary Cilium, University Hospital Limerick Annual Research Symposium, Limerick, Ireland, 2014 Conference Paper, 2014

Brady, R.T., O'Brien, F.J., Hoey, D.A., , Mechanically Stimulated Osteocytes and Osteoblasts Direct Stem Cell Migration and Proliferation: an in vitro Study, 20th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Limerick, Ireland, 2014 Conference Paper, 2014

Labour, M.N., Cavaignac, M., deBarra, E., Hoey, D.A., , Development of a composite poly-lactic acid / calcium phosphate electrospun material for bone tissue engineering, University Hospital Limerick Annual Research Symposium, Limerick, Ireland, 2014 Conference Paper, 2014

Eichholz, K.F., Hoey, D.A., , Fabrication of in-vitro micro-vessels for the study of cardiovascular mechanobiology, 20th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Limerick, Ireland, 2014 Conference Paper, 2014

Stavenschi, E., Hoey, D.A, A simple in-vitro fluid flow stimulation platform to enhance osteogenesis of human mesenchymal stem cells, University Hospital Limerick Annual Research Symposium, Limerick, Ireland, 2014 Conference Paper, 2014

Hoey, D.A., Chen, J.C., Jacobs, C.R., , Primary Cilia of Bone Marrow Cells Mediate Mechanically Induced Bone Formation, 20th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Limerick, Ireland, 2014 Conference Paper, 2014

Meaney, C., Kiely, P., Hoey, D.A., , Investigating the Incidence and Role of Primary Cilia in Cancer Models, University Hospital Limerick Annual Research Symposium, Limerick, Ireland, 2014 Conference Paper, 2014

Brady, R.T., O'Brien, F.J., Hoey, D.A., , Novel mechanisms in loading-induced bone formation, University Hospital Limerick Annual Research Symposium, Limerick, Ireland, 2013 Conference Paper, 2013

Brady, R.T., McCoy, R., Hoey, D.A., O'Brien, F.J., , The Combined Effect of Substrate Stiffness and Fluid Flow in Directing Osteogenic Differentiation, 19th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Enfield, Ireland, 2013 Conference Paper, 2013

Eichholz, K.F., Hoey, D.A., , Fabrication of in-vitro micro-vessels for the study of cardiovascular mechanobiology, University Hospital Limerick Annual Research Symposium, Limerick, Ireland, 2013 Conference Paper, 2013

Hoey, D.A., , Primary Cilium-Mediated Stem Cell Mechanobiology in Bone, University Hospital Limerick Annual Research Symposium, Limerick, Ireland, 2013 Conference Paper, 2013

Brady, R.T., McCoy, R., Hoey, D.A., O'Brien, F.J., Elucidation Of The Role Of Biophysical Environment In Directing Differentiation Of Stems Cells, Royal College of Surgeons in Ireland Research Day, Dublin, Ireland, 2013 Conference Paper, 2013

Kelly, N., Hoey, D.A., Walsh, M.T., Flood, H., , Prostate tissue stiffness can be correlated to severity of symptoms in Benign Prostatic Hyperplasia, University Hospital Limerick Annual Research Symposium, Limerick, Ireland, 2013 Conference Paper, 2013

Brady, R.T., Hoey, D.A., O'Brien, F.J., , Mechanically Stimulated Osteocytes Secrete Soluble Factors Which Alter Mesenchymal Stem Cell Proliferation and Migration, 18th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Belfast, Ireland, 2012 Conference Paper, 2012

Brady, R.T., O'Brien, F.J., Hoey, D.A., , Mechanically Stimulated Osteocyte Conditioned Media Alters Mesenchymal Stem Cell Proliferation and Migration, Royal College of Surgeons in Ireland Research Day, 2012 Conference Paper, 2012

Hoey, D.A., Jacobs, C.R., , Human Mesenchymal Stem Cells Utilize the Primary Cilium as a Mechanosensor during Oscillatory Fluid Flow Driven Osteogenic Differentiation, Columbia University Stem Cell Day, New York, US, 2011 Conference Paper, 2011

Hoey, D.A., O'Reilly, P, Taylor, D., , Fatigue behaviour of PMMA vs PMMA based bone cement, Proceedings of the 14th Annual Conference of the section of Bioengineering of the Royal Academy of Medicine in Ireland, Sligo, 2008 Conference Paper, 2008

Hoey D, Taylor D and O'Reilly P, The role of defects in the fatigue strength of bone cement, Bioengineering in Ireland 13, Enniskillen, Northern Ireland, January 2007, edited by Orr J , RAMI, 2007, pp53- Conference Paper, 2007

Hoey D, Taylor D and O'Reilly P, Predicting fatigue failure in bone cement, Bioengineering in Ireland 12, Galway, January 2006, edited by P.McHugh , Royal Academy of Medicine in Ireland, 2006, pp43- Conference Paper, 2006

Research Expertise

Description

The focus of my lab (www.hoeylab.com) is to delineate the importance of mechanics in human physiology and utilise this fundamental information to inform the development of novel strategies for tissue regeneration. Core Research areas: . Musculoskeletal Biomechanics, Mechanobiology, and Mechanotransduction . Primary Cilium Biology, Mechanobiology, and Ciliotherapies . Extracellular Vesicles, Exosomes, and Related Therapies . Biofabrication, 3D Printing, Melt Electrowriting . I have secured considerable competitive research funding in the form of multiple European Research Council grants (Starting-2013; PoC-2018) and a Science Foundation Ireland (SFI) Frontier's grant (2020). This funding facilitated a transformational research program where I am uncovering the role of mechanics and particularly the role of a cellular organelle, the cilium, in bone mechanoadaptation. This very successful program has led to a series of high impact papers (Stem Cells-JIF:6.94, Nature Aging) and established me as a world leader in cilia mechanobiology, best reflected by the many invited keynotes/awards (Gordon conference). This funding has also formed the basis of a new research program on extracellular vesicles (SFI Frontiers-2020). Utilising mechanics to prime the regenerative properties of vesicles, I am discovering novel mechanisms by which exercise promotes health, that I am harnessing therapeutically. I have published extensively in the leading journals in this area (J.EV-JIF:25.8) and recently delivered an invited talk (WebEVTalk). I am also an Investigator (FI) in the world leading SFI AMBER centre. I have collaborative funding with the large multinational company J&J, stemming from exciting technological advancements I have made in Melt Electrowriting. Through fundamental discovery (Advanced Healthcare Materials-JIF:9.93) and commercial exploitation (3xIDFs) I have established myself as pioneer in this area as demonstrated by an invited talk at TERMIS-EU. My research has defined important and transformational research questions across multiple evolving core areas that has resulted in novel therapies and has established me as an international leader in my field.

Projects

  • Title
    • Mechanically Activated Extracellular Vesicles as a Multi-targeted Therapy to Enhance Bone Regeneration
  • Summary
    • Every 3 seconds a person suffers an osteoporosis-related bone fracture, resulting in significant morbidity, mortality, and health-care costs. Osteoporosis arises when there is an imbalance between resorption and formation resulting in net bone loss. Current therapeutics are limited in efficacy and by side-effects. A potent regulator of bone formation and repair is physical loading. Bone cells sense mechanical stimuli and subsequently coordinate net bone gain by secreting multitargeted paracrine factors. Recent findings by the applicant indicates that these factors are delivered via extracellular vesicles (EV), which are membrane-bound cargoes that facilitate cell-cell communication. Therefore, this research aims to develop a novel EV-based multitargeted therapy that mimics the beneficial effects of physical exercise, by inhibiting osteoclastogenesis and bone resorption, while also enhancing angiogenesis, osteogenesis and bone formation. The identification of EVs and associated components as central to loading-induced bone anabolism will lead to the direct manipulation of multiple cell types via mechanically-activated EV based therapeutics. These therapeutics would therefore be used as a novel multitargeted strategy to treat osteoporosis. Furthermore, these EVs will be incorporated into scaffolds, generating innovative mechanobiomimetic materials for bone regeneration. These therapies have the potential to transform how millions suffering from osteoporosis and bone defects are treated.
  • Funding Agency
    • Science Foundation Ireland
  • Date From
    • 01/01/2020
  • Date To
    • 31/12/2023
  • Title
    • Microphysiological models of human bone: A new platform for investigation and drug screening.
  • Summary
    • This project will combine expertise within the applicant's team, along with collaborators in the University of Birmingham, to develop new materials and cell culture platforms which will facilitate the recapitulation of human bone physiology in a dish, focusing on the establishment of a human osteocyte network within a vascularised and mineralised tissue that is capable of undergoing remodelling, in response to both hormonal and physical cues. This first of its kind platform will then be used to screen novel therapeutics which are being developed by the applicant in the form of extracellular vesicles (EVs) and G-protein coupled receptors (Gpr161) as treatments for osteoporosis and will compare against commercial standards.
  • Funding Agency
    • Trinity College Dublin
  • Date From
    • 01/01/2020
  • Date To
    • 31/12/2023
  • Title
    • How to Promote Spinal Fusion in Osteoporosis: A Bioprinting Strategy
  • Summary
    • Spinal fusion is a surgical procedure whereby two or more vertebrae are joined together to grow into a single solid bone. Indications for fusion procedures include fractured vertebrae, tumour resection and degenerative spinal conditions. The incidence of spinal fusion surgery is rising dramatically worldwide, in part due to an ever increasing aging population. In the setting of osteoporosis, which is also linked to aging, spinal fusion is associated with significant technical challenges, morbidity and healthcare costs. More than 200 million people are suffering from osteoporosis worldwide. Insufficiency fractures, failure of fusion and instrumentation failure are some of the complications encountered. Furthermore, the revision surgery required to address these complications is a major surgical undertaking associated with significant risk and long-term functional restrictions. Our hypothesis is that the increased incidence of complications is secondary to a defective stem cell population, and this may be therapeutically addressed through the use of novel bioprinted materials. We will first track the stem cell contributions to spinal fusion using a transgenic mouse model and compare how this is altered in an induced osteoporotic model (Workpackage 1). In workpackage 2 we will characterise stem cells isolated from osteoporotic and non-osteoporotic patients undergoing spinal fusion surgery. We will compare the regenerative potential of these cells to patient spinal fusion outcomes with the hypothesis that those patients with poor fusion outcomes will correlate with the quality of stem cells. Finally, we will attempt to use novel bioprinted materials previously optimised to enhance stem cell based bone formation to increase spinal fusion rates in the mouse model described earlier. Therefore this project will identify stem cells as critical to spinal fusion success and will demonstrate the applicability of novel materials to target this cell to enhance fusion in osteoporosis addressing the currently unacceptable high failure rates of this procedure.
  • Funding Agency
    • Irish Research Council
  • Date From
    • 01/09/2019
  • Date To
    • 31/08/2022
  • Title
    • Primary Cilium Mediated Mesenchymal Stem Cell Mechanobiology in Bone
  • Summary
    • Every 30 seconds a person suffers an osteoporosis-related bone fracture in the EU, resulting in significant morbidity, mortality, and health-care costs estimated at €36 billion annually. Current therapeutics target bone resorbing osteoclasts, but these are associated with severe side effects. Osteoporosis arises when mesenchymal stem cells (MSC) fail to produce sufficient numbers of bone forming osteoblasts. A key regulator of MSC behaviour is physical loading, yet the mechanisms by which MSCs sense and respond to changes in their mechanical environment are virtually unknown. Primary cilia are nearly ubiquitous 'antennae-like' cellular organelles that have very recently emerged as extracellular mechano/chemo-sensors and thus, are strong candidates to play an important role in regulating MSC responses in bone. However, to date, research on the stem cell primary cilium is almost non-existent. Therefore, the objective of this research program is to determine the role of the understudied primary cilium and associated molecular components in the osteogenic differentiation and recruitment of human MSCs in loading-induced bone adaptation. This will be achieved through ground breaking in vitro and in vivo techniques developed by the applicant. The knowledge generated in this proposal will represent a profound advance in our understanding of stem cell mechanobiology. In particular, the identification of the cilium and associated molecules as central to stem cell behaviour will lead to the direct manipulation of MSCs via novel cilia-targeted therapeutics that mimic the regenerative influence of loading at a molecular level. These novel therapeutics would therefore target bone formation rather than resorption, providing an innovative alternative path to treatment, resulting in an improved supply of bone forming cells, preventing osteoporosis. Furthermore, these novel therapeutics will be incorporated into biomaterials generating novel bioactive osteoinductive scaffolds. These advances will not only improve quality of life for the patient but will significantly reduce the financial burden of bone loss diseases in the EU.
  • Funding Agency
    • European Research Council
  • Date From
    • 01/11/2013
  • Date To
    • 31/10/2018
  • Title
    • Osteoprogenitor Regulation in Loading-induced Bone Formation: An Alternative Approach to Treating Osteoporosis
  • Summary
    • Every 30 seconds a person suffers an osteoporosis-related bone fracture, resulting in significant morbidity, mortality, and health-care costs estimated at 36billion euro annually. Current therapeutics target osteoclasts but are associated with severe side effects. Osteoporosis arises when stem cells (MSCs) fail to produce sufficient numbers of osteoblasts. A key regulator of MSC behaviour is loading, yet the mechanisms by which MSCs proliferate, differentiate and are recruited to sites of loading to replace exhausted osteoblasts remain elusive. Osteocytes are ideally numbered and positioned to be sensors and regulators of bone formation. However research on osteocyte-MSC signalling is almost non-existent. Therefore the objective of this research project is to determine the role of the osteocyte and associated signalling mechanisms in regulating MSC contributions to bone formation and to develop novel 'Bone-on-a-Chip' microfluidic platforms for the validation of novel anabolic therapeutics for osteoporosis.
  • Funding Agency
    • Irish Research Council
  • Date From
    • 2014
  • Date To
    • 2018

Keywords

BIOMATERIALS; BIOMECHANICS; Biomechanics, Biomedical Engineering; BONE ABNORMALITIES; BONE ADAPTATION; BONE CEMENT; BONE MASS; BONE METASTASIS; BONE REGENERATION; bone remodelling and repair; CELL BIOMECHANICS; MECHANOBIOLOGY; MESENCHYMAL STEM CELLS; Promotion of Bone Health throgh Exercise; Stem Cell Biology

Recognition

Awards and Honours

Co-author on paper shortlisted for the New Investigator Recognition Award at the Orthopaedic Research Society Meeting 2022 2022

Fellow of Trinity College Dublin (FTCD) 2021

Senior author on paper awarded 1st prize in the 'Biomechanics' section at the Bioengineering in Ireland Conference, Dublin, Ireland. Awarded to Dr. Mathieu Riffault 2020

Science Foundation Ireland Frontiers for the Future Project Grant (€622K). 2020

European Research Council Proof of Concept Award (€150K) 2018

Co-author on paper awarded 1st prize in the student poster competition at the 2018 Health Research Institute Conference, University of Limerick, Ireland. Awarded to Ms. Anushree Dwivedi. 2018

Co-author on paper awarded 'Lab on a Chip Poster Prize' at Organ on a Chip and Tissue on a Chip Conference, Rotterdam, The Netherlands. Awarded to Mr. Ian Whelan 2018

Senior author on paper awarded 2nd prize in the student competition at the 2017 Bioengineering in Ireland Conference, Belfast, Northern Ireland. Awarded to Ms. Michele Corrigan. 2017

Senior author of paper awarded 1st prize at the Sir Bernard Crossland Symposium (Kian Eichholz) 2016

Senior author on paper awarded 2nd prize at the Thesis in 3 Trinity Centre for Bioengineering competition. Awarded to Mr. Kian Eichholz. 2016

Co-author on paper awarded 3rd prize in the poster competition at the 2016 UL/NUIG Research Day. Awarded to Ms. Gillian Johnson. 2016

Senior author on paper awarded 1st prize at the Sir Bernard Crossland Symposium, Queens University Belfast, Northern Ireland. Awarded to Mr. Kian Eichholz. 2016

Senior author on paper shortlisted for the 'New Investigator Recognition Award' at the 61st Orthopaedic Research Council Meeting, Las Vegas, U.S. 2015

Marie-Skłodowska Curie COFUND Excellence award 2015

University of Limerick Award for Research Excellence (Early Career) 2014

Co-author on paper awarded the 'New Investigator Recognition Award' at the 60th Orthopaedic Research Council Meeting, New Orleans, U.S. 2014

Senior author on paper awarded 'Best poster in the Technology and Health Section' at the 2nd University Hospital Limerick Research Symposium, Limerick, Ireland. 2014

Senior author on thesis awarded 'Best Undergraduate Final Year Project' awarded to Mr. Kian Eichholz at the Materials and Surface Science Institute Research Day, University of limerick, Limerick, Ireland. 2014

Senior author on paper awarded 'Best poster in the Technology and Health Section' of the Inaugural University Hospital Limerick Research Symposium, Limerick, Ireland. 2013

Co-author on paper awarded the 'Harold M. Frost Young Investigator Award' at the 43rd International Bone and Mineral Society Sun Valley Workshop, Idaho, U.S. 2013

Co-author on paper awarded the 'ASBMR Young Investigator Award' at the American Society for Bone and Mineral Research, Baltimore, U.S. 2013

European Research Council Starting Grant (€1.45M) 2013

Finalist for the 'New Investigator Recognition Award' at the Orthopaedic Research Society 2012

'Symington Bequest Fund Award' from the Anatomical Society, U.K. 2012

2nd prize in the poster competition at the 4th Mechanics of Biomaterials and Tissues Meeting, Hawaii, U.S. 2011

'Outstanding Junior Investigator' at the 4th New York Skeletal Biology and Medicine Meeting, New York, U.S. 2011

'Short talk travel award' at the Society for Physical Regulation in Biology and Medicine, Florida, U.S. 2011

'Best presentation in the Postdoctoral Category' at the Society for Physical Regulation in Biology and Medicine, Florida, U.S. 2011

'Best presentation in the Postdoctoral Category' at the Society for Physical Regulation in Biology and Medicine, Arizona, U.S. 2010

'IRCSET Marie-Curie INSPIRE fellowship in Science, Engineering and Technology', jointly from the Irish Research Council and Marie-Cure Foundation. 2009

Senior author on paper awarded the 'Engineers Ireland Biomedical Research Medal' from the Society of Engineers Ireland. Awarded to Mr. Kian Eichholz 2009

1st prize in the PhD poster competition at the American Society of Mechanical Engineers Bioengineering Division Summer Conference, Florida, U.S. 2008

'Engineers Ireland Biomedical Research Medal' from the Society of Engineers Ireland. 2008

1st prize at the Sir Bernard Crossland Symposium, National University of Ireland: Galway. 2007

Memberships

Tissue Engineering and Regenerative Medicine Society (TERMIS) 2015 – Present

Orthopaedic Research Society (ORS) 2010 – Present

Marie-Curie Fellow Association 2015 – Present

Engineers Ireland (Committee member and Treasurer of the Biomedical Division) 2006 – 2020

European Society of Biomechanics (ESB) 2013 – Present