Dr. Michael Monaghan
Ussher Assistant Professor, Mechanical, Manuf & Biomedical Eng
Biography
Dr. Michael Monaghan is an Ussher Assistant Professor in Biomedical Engineering at Trinity College Dublin, the University of Dublin. His group's primary research areas are in biomaterials (in particular electroconductive biomaterials), advanced processing and rational design of biomaterials, cardiac tissue regeneration, and real-time imaging of extracellular matrix components and metabolism in differentiation and disease. He leads a number of interdiciplinary projects between other academic groups and industry ranging from immunometabolism, evaluation of fibrosis, biomaterial synthesis and induced pluripotent stem cell derivitisation of cardiac organoids.
Dr. Monaghan performed his Postdoctoral research in Germany as a recipient of a prestigious Marie Curie Individual Fellowship. His research was coordinated between the Department of Cell and Tissue Engineering in the Fraunhofer Institute for Interfacial Engineering and Biotechnology and the Department of Women's Health in University Clinic Tubingen. During this period he has published a number of key papers in the field of human valvulogenesis, embryonic stem cell research, cardiomyocyte differentiation, biomaterials and non-invasive optical characterisation (Raman microspectroscopy, fluorescent lifetime imaging (FLIM), multiphoton and second harmonic generation (SHG) imaging). Dr. Monaghan received both his B.Eng (Biomedical) and Ph.D. (Biomedical Engineering) from the National University of Ireland Galway (NUIG). During his Ph.D. Dr. Monaghan received a number of research awards such as travel awards from the European Molecular Biology Organisation (EMBO) and the German Academic Exchange Service (DAAD), and the 2015 Julia Polak European Doctorate Award in recognition of the achievements made during his Ph.D.
Dr. Monaghan is actively involved in the tissue engineering and regenerative medicine international society (TERMIS); previously as Chair of the EU Student and Young Investigator Section, and recently promoted full EU Council Member. He is council member of Matrix Biology Ireland and acts as treasurer of this society. More details of his groups resources and research can be found on the groups website.
Lab Website: www.monaghanlab.com
Publications and Further Research Outputs
Peer-Reviewed Publications
Fitzgerald, H.K.; O'Rourke, S.A.; Desmond, E.; Neto, N.G.B.; Monaghan, M.G.; Tosetto, M.; Doherty, J.; Ryan, E.J.; Doherty, G.A.; Nolan, D.P.; Fletcher, J.M.; Dunne, A, The Trypanosoma brucei-Derived Ketoacids, Indole Pyruvate and Hydroxyphenylpyruvate, Induce HO-1 Expression and Suppress Inflammatory Responses in Human Dendritic Cells, Antioxidants, 11, 2022, p164-
Saha, Uttariyo, Nairn, Rory, Keenan, Orla, Monaghan, Michael G., A Deeper Insight into the Influence of the Electric Field Strength When Melt"Electrowriting on Non"Planar Surfaces, Macromolecular Materials and Engineering, 2021, p2100496
Fitzgerald H., Williams D. G., Campbell N. K., Barry P, O'Rourke S. A. Neto N., Monaghan M. G., Nolan D. P., Dunne A, Aromatic ketoacids represent novel therapies for autoimmune diseases through activation of the Nrf2/HO-1 pathway and suppression of pro-inflammatory responses in primary human immune cells, British Society for Immunology 2021 Congress, Edinburgh, 30th November, 2021
Perottoni S.*, Neto N., di Nitto C., Dmitriev R., Raimoundi M.T., Monaghan M, ., Intracellular label-free detection of mesenchymal stem cell metabolism within a perivascular niche-on-a-chip, European Society for Biomechanics, Milan, Italy (and online), 11-14th July, 2021
Floudas A, Neto N, Orr C, Canavan M, Gallagher P, Hurson C, Monaghan MG, Nagpar S, Mullan RH, Veale DJ, Fearon U., Loss of balance between protective and pro-inflammatory synovial tissue T-cell polyfunctionality predates clinical onset of rheumatoid arthritis., Annals of the rheumatic diseases, 2021
Meenakshi Suku, Lesley Forrester, Manus Biggs, Michael G. Monaghan, Resident macrophages and their potential in cardiac tissue engineering, Tissue Engineering Part B Reviews, 2021
Neto N., Perottoni S., di Nitto C., Dmitriev R., Raimoundi M.T., Monaghan M, ., Intracellular label-free detection of mesenchymal stem cell metabolism within a perivascular niche-on-a-chip, Termis Workshop: Cell-Matrix Interaction and Mechanobiology in Regenerative Medicine, Online, 11-12th June, 2021
Perottoni S, Neto NGB, Di Nitto C, Dmitriev RI, Raimondi MT, Monaghan MG., Intracellular label-free detection of mesenchymal stem cell metabolism within a perivascular niche-on-a-chip., Lab on a chip, 21, (7), 2021, p1395-1408
Neto N., O'Rourke S., Zhang M., Dunne A., Monaghan M, Single-cell metabolic profiling of macrophage polarization by fluorescence lifetime imaging microscopy (FLIM), 6th European Congress for Immunology Meeting, Online, 1-4th September, 2021
Solazzo M, Monaghan MG., Structural crystallisation of crosslinked 3D PEDOT:PSS anisotropic porous biomaterials to generate highly conductive platforms for tissue engineering applications., Biomaterials science, 9, (12), 2021, p4317-4328
Old Drugs, New Tricks - Redefining Therapeutic Strategies For Tissue Regeneration., Advanced Drug Delivery Reviews- Elsevier, 2021, -
ORourke S., Neto N., Devilly E., Shanley L., Dunne A., Monaghan M. G, Targeting metabolism in cardiovascular disease: investigating metabolic reprogramming of macrophages in atherosclerosis as a novel therapeutic strategy, British Society for Immunology 2021 Congress, Edinburgh, 30th November, 2021
Perottoni S.*, Neto N., di Nitto C., Dmitriev R., Raimoundi M.T., Monaghan M, Intracellular label-free detection of mesenchymal stem cell metabolism within a perivascular niche-on-a-chip, European Society for Biomechanics, Milan, Italy (and online), 11-14th July, 2021
Coulter, Fergal B., Levey, Ruth E., Robinson, Scott T., Dolan, Eimear B., Deotti, Stefano, Monaghan, Michael, Dockery, Peter, Coulter, Brian S., Burke, Liam P., Lowery, Aoife J., Beatty, Rachel, Paetzold, Ryan, Prendergast, James J., Bellavia, Gabriella, Straino, Stefania, Cianfarani, Francesca, Salamone, Monica, Bruno, Carmelo M., Moerman, Kevin M., Ghersi, Giulio, Duffy, Garry P., O'Cearbhaill, Eoin D., Soft Tissue Implants: Additive Manufacturing of Multi"Scale Porous Soft Tissue Implants That Encourage Vascularization and Tissue Ingrowth (Adv. Healthcare Mater. 14/2021), Advanced Healthcare Materials, 10, (14), 2021, p2170063
Shanley L., Mahon O. R., O'Rourke S. A., Neto N., Monaghan M. G., Kelly D., Dunne A, Particle size alters macrophage metabolism: Implications for bone tissue engineering, British Society for Immunology 2021 Congress, Edinburgh, 30th November, 2021
Floudas A., Neto N., Vivianna M., Murray K., Moran B., Monaghan M., Candice L., Mullan R.H., Krishna V., Nagpal S., Veale D.J., Fearon U, Pathogenic, Glycolitic PD-1+ cells accumulate in the Hypoxic RA joint, 8th Annual Meeting of the International Cytokine and Interferon Society, Seattle, USA, 1-4th November 2020, 2020
Olvera D, Monaghan MG, Electroactive biomaterial-based biosensors for detection and drug delivery , Advanced Drug Delivery Reviews, 2020
Seeing Is Believing: Noninvasive Microscopic Imaging Modalities for Tissue Engineering and Regenerative Medicine in, editor(s)Heinz Redl, Jeff Gimble, Darja Marolt Presen, Richard Oreffo and Susanne Wolbank , Tissue Engineering and Regeneration. Cell Engineering and Regeneration, Switzerland, Springer, 2020, ppAccepted- - In Press, [Neto N, Dmitriev R, Monaghan MG]
Floudas A, Neto N, Marzaioli V, Murray K, Moran B, Monaghan MG, Low C, Mullan RH, Rao N, Krishna V, Nagpal S, Veale DJ, Fearon U., Pathogenic, glycolytic PD-1+ B cells accumulate in the hypoxic RA joint., JCI insight, 5, (21), 2020
Olvera D., Sohrabi Molina M., Hendy G., Monaghan M.G., Electroconductive Melt Electrowritten Patches Matching the Mechanical Anisotropy of Human Myocardium, Advanced Functional Materials, 2020
Extrusion-based additive manufacturing techniques for biomedical applications in, editor(s)Dermot Brabazon , Encyclopedia of Materials: Composites, Philadelphia, Elsevier, 2020, ppIn Press - [Tadayyon G, Kelly DJ, Monaghan MG]
Ozaki, Ema, Gibbons, Luke, Neto, Nuno GB, Kenna, Paul, Carty, Michael, Humphries, Marian, Humphries, Pete, Campbell, Matthew, Monaghan, Michael, Bowie, Andrew and Doyle, Sarah L, SARM1 deficiency promotes rod and cone photoreceptor cell survival in a model of retinal degeneration, Life Science Alliance, 3, (5), 2020
Ukkelman IA, Neto N, Papkovsky DB, Monaghan MG, Dmitriev RI., A deeper understanding of intestinal organoid metabolism revealed by combining fluorescence lifetime imaging microscopy (FLIM) and extracellular flux analyses., Redox biology, 30, 2020, p101420
Neto N., Greaney C., Olvera D., Duffy C., Hoey D., Monaghan M.G, Discerning the Effect of Substrate directed differentiation on Mesenchymal Stromal Cell Metabolic Activity using FLIM, 25th Bioengineering in Ireland 2019, , Limerick Ireland, 18-19th January, 2019
Solazzo M, O'Brien FJ, Nicolosi V, Monaghan MG., The rationale and emergence of electroconductive biomaterial scaffolds in cardiac tissue engineering., APL Bioeng, 3, (4), 2019, p041501
O'Rourke SA, Dunne A, Monaghan MG., The Role of Macrophages in the Infarcted Myocardium: Orchestrators of ECM Remodeling., Frontiers in cardiovascular medicine, 6, 2019, p101
O'Rourke S., Dunne A., Monaghan, M.G, The Influence of Electroconductive Materials on Macrophage Phenotype, 25th Bioengineering in Ireland 2019, Limerick, Ireland, 2019
Solazzo, M., Wong S., Zhussupbekova A., Krukiewicz K., Fleisher K., Biggs M. J. P., Monaghan, M. G. , Engineered electroconductive biomaterial platforms for cardiac tissue engineering applications, Sir Bernard Crossland Symposium 2019, Belfast, 17-18th April, 2019
Solazzo M, Krukiewicz K, Zhussupbekova A, Fleischer K, Biggs MJ, Monaghan MG., PEDOT:PSS interfaces stabilised using a PEGylated crosslinker yield improved conductivity and biocompatibility., J Mater Chem B, 7, (31), 2019, p4811-4820
Solazzo, M., Zhussupbekova A., Krukiewicz K., Fleisher K., Biggs M. J. P., Monaghan, M. G, Minimally invasive 3D electroconductive biomaterial platforms for cardiac tissue engineering applications, Tissue Engineering and Regenerative Medicine International Society (TERMIS) EU Chapter meeting, Rhodes, Greece, 27- 31st of May, 2019
Dolan, E.B. and Hofmann, B. and de Vaal, M.H. and Bellavia, G. and Straino, S. and Kovarova, L. and Pravda, M. and Velebny, V. and Daro, D. and Braun, N. and Monahan, D.S. and Levey, R.E. and O'Neill, H. and Hinderer, S. and Greensmith, R. and Monaghan, M.G. and Schenke-Layland, K. and Dockery, P. and Murphy, B.P. and Kelly, H.M. and Wildhirt, S. and Duffy, G.P., A bioresorbable biomaterial carrier and passive stabilization device to improve heart function post-myocardial infarction, Materials Science and Engineering C, 103, (109751), 2019
Solazzo, M.*, Monaghan, M. G, Minimally invasive 3D electroconductive biomaterial platforms for cardiac tissue engineering applications, 25th Bioengineering in Ireland 2019, Limerick, Ireland, 18-19th January, 2019
Dolan E. B., Varela C. E., Mendez K., Whyte W., Levey R. E., Robinson S. T., Maye E., Oâ Dwyer J., Beatty R., Rothman A., Fan Y., Hochstein J., Rothenbucher S. E., Wylie R., Starr J. R., Monaghan M., Dockery P., Duffy G. P., Roche E. T., An actuatable soft reservoir modulates host foreign body response , Sci. Robotics , 4 , (33 ), 2019, peaax7043 -
Shen N, Riedl JA, Carvajal-Berrio DA, Davis Z, Monaghan MG, Layland SL, Hinderer S, Schenke-Layland K. , A flow bioreactor system compatible with real-time two-photon fluorescence lifetime imaging microscopy., Biomedical Materials, 13, 2018, p024101-
Solazzo, M., Monaghan, M. G, An electroconductive 3D porous PEDOT:PSS scaffold for cardiac tissue engineering applications, 24th Bioengineering in Ireland 2018, Dublin, 26-27th January, 2018
Monaghan M.G, Holeiter M, Brauchle E, Layland S.L, Lu Y, Deb A, Pandit A, Nsair A, Schenke-Layland K, Exogenous miR-29B delivery via a hyaluronan-based injectable system yields functional maintenance of the infarcted myocardium, Tissue Engineering Part A, 24, 2018, p57 - 67
Neto N., Greaney C., Olvera D., Duffy C., Hoey D., Monaghan M.G, Discerning the Effect of Substrate directed differentiation on Mesenchymal Stromal Cell Metabolic Activity using FLIM, Matrix Biology Ireland Galway Meeting, Galway, Ireland, 21st November , 2018
Lotz C, Schmid F.F, Oechsle E, Monaghan M, Walles H, Groeber-Becker F.K, A crosslinked collagen hydrogel matrix resisting contraction to facilitate full-thickness skin equivalents, ACS Appl. Mater. Interfaces, 9, 2017, p20417 - 20425
Lakner P.H*, Monaghan M. G*, M, Möller Y, Olayioye M.A, Schenke-Layland K. , Applying a phasor approach analysis of multiphoton fluorescence lifetime imaging microscopy measurements to probe the metabolic activity of three-dimensional in vitro cell culture models., Nature Scientific Reports, 7, 2017, p42730-
Monaghan M.G, Holeiter M, Layland S.L, Schenke-Layland K, Cardiomyocyte generation from somatic sources " current status and future directions , Current Opinion in Biotechnology , 40, 2016, p49 - 55
Lakner P, Möller Y, Olayioye M, Brucker S.Y, Schenke-Layland K, Monaghan M.G., Multiphoton Microscopy in the Biomedical Sciences XVI, Proc SPIE9712, SPIE BioS Multiphoton Microscopy in the Biomedical Sciences XVI, San Francisco, 14th March 2016, edited by Ammasi Periasamy; Peter T. C. So; Karsten König , 2016, pp97120X
Brauchle E, Knopf A, Bauer H, Shen N, Linder S, Monaghan MG, Ellwanger K, Layland SL, Brucker SY, Nsair A, Schenke-Layland K, Non-invasive Chamber-Specific Identification of Cardiomyocytes in Differentiating Pluripotent Stem Cells., Stem cell reports, 6, (2), 2016, p188-99
Lakner P, Möller Y, Olayioye M, Brucker S.Y, Schenke-Layland K, Monaghan, M.G, A Phasor approach analysis of multiphoton FLIM measurements of three-dimensional cell culture models, SPIE Photonics West meeting , San Francisco, USA, 13-18th February , 2016
Monaghan MG, Linneweh M, Liebscher S, Van Handel B, Layland SL, Schenke-Layland K, Endocardial-to-mesenchymal transformation and mesenchymal cell colonization at the onset of human cardiac valve development., Development (Cambridge, England), 143, (3), 2016, p473-82
Molina-Arce C, Brauchle E, Monaghan M.G, Holeiter M, Schenke-Layland K. , Characterization of extracellular matrix in murine scar tissues with near infrared Raman microspectroscopy, Innovation Match MX, Guadalajara, Mexico, 6-8th April , 2016
Monaghan MG, Kroll S, Brucker SY, Schenke-Layland K, Enabling Multiphoton and Second Harmonic Generation Imaging in Paraffin-Embedded and Histologically Stained Sections., Tissue engineering. Part C, Methods, 22, (6), 2016, p517-23
Browne, S., Monaghan, M.G., Brauchle, E., Berrio, D.C., Chantepie, S., Papy-Garcia, D., Schenke-Layland, K., Pandit, A., Modulation of inflammation and angiogenesis and changes in ECM GAG-activity via dual delivery of nucleic acids, Biomaterials, 69, 2015, p133-147
Monaghan M, Brauchle E, Carvajal Berrio D, Deb A, Pandit A, Nsair A, Schenke-Layland K, Biomaterial delivery of exogenous miR-29b to the infarcted myocardium yields improved myocardial recovery, the World Congress of the Tissue Engineering and Regenerative Medicine International Society, Boston, MA, USA, 8-11th September , 2015
Optical reprogramming and opticalcharacterization of cells using femtosecond lasers in, editor(s)König K, Ostendorf A , Biomedical Applications in Optically-Induced Nanostructures for Biomedical and Technical Applications, De Gruyter , 2015, pp159 - 178, [1. Uchugonova A, Augspurger C, Monaghan M, Schenke-Layland K, Konig K]
Dash, B.C., Thomas, D., Monaghan, M., Carroll, O., Chen, X., Woodhouse, K., O'Brien, T., Pandit, A., An injectable elastin-based gene delivery platform for dose-dependent modulation of angiogenesis and inflammation for critical limb ischemia, Biomaterials, 65, 2015, p126-139
Groeber, F., Engelhardt, L., Egger, S., Werthmann, H., Monaghan, M., Walles, H., Hansmann, J., Impedance Spectroscopy for the Non-Destructive Evaluation of In Vitro Epidermal Models, Pharmaceutical Research, 32, (5), 2015, p1845-1854
Schesny, M.K., Monaghan, M., Bindermann, A.H., Freund, D., Seifert, M., Eble, J.A., Vogel, S., Gawaz, M.P., Hinderer, S., Schenke-Layland, K., Preserved bioactivity and tunable release of a SDF1-GPVI bi-specific protein using photo-crosslinked PEGda hydrogels, Biomaterials, 35, (25), 2014, p7180-7187
Maurer M, Monaghan M, Möller Y, Olayioye M, Schenke-Layland K, Single-cell transfection within a 3D tumor model using optoporation, Biomedical Engineering Society Annual Meeting, San Antonio, Texas, USA, 22-25th October, 2014
Monaghan M, Browne S, Schenke-Layland K, Pandit A, A Collagen-based Scaffold Delivering Exogenous microRNA-29B to Modulate Extracellular Matrix Remodeling , Meeting of the German Matrix Biology Society (DGMB), Regensburg, Germany, 20-24th March , 2014
Monaghan, Michael, Browne, Shane, Schenke-Layland, Katja, Pandit, Abhay, A Collagen-based Scaffold Delivering Exogenous MicroRNA-29B to Modulate Extracellular Matrix Remodeling, Molecular Therapy, 22, (4), 2014, p786-796
Monaghan, M., Augspurger, C., Brauchle, E., Lakner, P., Breunig, G., Konig, K., Schenke-Layland, K., Fluorescent live time imaging from pluripotency to differentiation in mouse embryonic stem cells reveals endogenous autofluorescence profiles, Journal of Tissue Engineering and Regenerative Medicine, 8, 2014, p300-301
Michael Monaghan, An injectable collagen scaffold delivering exogenous microRNA as a therapy to modulate extracellular matrix remodelling , NUI Galway, 2013
Energy Regeneration systems in cell free protein synthesis in vitro in, editor(s)Bethaz C, Li Puma V , New Research on Protein Synthesis, Nova Science Publishers, Inc, 2013, pp67 - 76, [2. Kahlig A, Schwedhelm I, Monaghan M, Thein M, Hansmann J]
Monaghan M, Browne S, Wang W, Pandit A, Delivery and efficacy of interfering RNA via a crosslinked collagen hydrogel in an excisional in vivo model and a human pre‐clinical model to reduce fibrosis, 3rd International Conference , Wurzburg, Germany, 23-25th May, 2012
Monaghan, M., Greiser, U., Wall, J.G., O'Brien, T., Pandit, A., Interference: An alteRNAtive therapy following acute myocardial infarction, Trends in Pharmacological Sciences, 33, (12), 2012, p635-645
Monaghan M, Browne S, Wang W, Pandit A, Delivery and efficacy of interfering RNA via a crosslinked collagen hydrogel in an excisional in vivo model and a human pre‐clinical model to reduce fibrosis, World Congress of Tissue Engineering and Regenerative Medicine International Society, Vienna, Austria, 5-8th September, 2012
Monaghan, M. Browne, S. Wang, W. Pandit, A.(ed.), Scaffold mediated non-viral inhibition of collagen type I and type III in cardiac fibroblasts, 2011
Monaghan, M. Holladay, C. Pandit, A.(ed.), A crosslinked collagen type i biomaterial reservoir for non-viral gene delivery in vivo, 2011
Monaghan, M., Pandit, A., RNA interference therapy via functionalized scaffolds, Advanced Drug Delivery Reviews, 63, (4), 2011, p197-208
Monaghan M, Greiser U, Cao H, Wang W, Pandit A, A ligand enhanced dendritic PEGylated poly (2-(dimethylamino) ethyl diacrylate) as a vehicle of microRNA, Drug Delivery and Translational Research, 2, 2011, p406 - 414
Monaghan M, Browne S, Wang W, Pandit A, Scaffold-mediated non-viral inhibition of collagen type I and type III in cardiac fibroblasts, European Society of Biomaterials Conference, Dublin, Ireland, 4-9th September , 2011
Monaghan M, Mathew A, Greiser U, Wang W, Pandit A, Scaffold mediated delivery of microRNA immunopolyplexes, European Society of Biomaterials Conference, Tampere, Finland, 11-15th September, 2010
Monaghan M, Mathew A, Greiser U, Wang W, Pandit A, Scaffold mediated delivery of microRNA immunopolyplexes, Tissue Engineering and Regenerative Medicine Conference, Galway, Ireland, 14-17th June, 2010
Dash B.C, Mahor S, Monaghan M, Woodhouse K.A, Pandit A, Elastin like protein based hollow spheres for gene delivery, European Society of Biomaterials Conference, Tampere, Finland, 11-15th September, 2010
Dash, B.C., Réthoré, G., Monaghan, M., Fitzgerald, K., Gallagher, W., Pandit, A., The influence of size and charge of chitosan/polyglutamic acid hollow spheres on cellular internalization, viability and blood compatibility, Biomaterials, 31, (32), 2010, p8188-8197
Dash B C, Mahor S, Monaghan M, Woodhouse K A, Pandit A, Elastin like protein based hollow spheres for gene delivery, Tissue Engineering and Regenerative Medicine Conference, Galway, Ireland, 14-17th June, 2010
Mathew A, Monaghan M, Wang W, Pandit A. , A linear-dendritic hybrid poly (2-(dimethylamino) ethyl methacrylate) as a highly effective gene delivery vector, the European Society of Biomaterials Conference, Tampere, Finland, 11-15th September, 2010
Kelly J.L, Doody J, Zeugolis D, Monaghan M, Kelly G, Devocelle M, Kilcoyne M, Joshi L, Pandit A, Collagen fibers modified with polysialic mimetic peptide are a suitable material for use as a synthetic peripheral nerve graft, Irish Association of Plastic Surgery Meeting, Dublin, IReland, 26th November, 2010
Matthew A, Réthoré G, Dash B C, Monaghan M, Pandit, A, Chitosan/polyglutamic acid nanospheres: DNA encapsulation and release, cellular uptake and blood interaction studies, UK Society for Biomaterials Conference, Antrim, UK, 2009
Réthoré G, Monaghan M, Dash B C, Pandit A, Chitosan-polyglutamic acid biodegradable nanospheres show a blood compatibility response that is dictated by size and surface charge, 2nd World Congress of the Tissue Engineering and Regenerative Medicine International Society, Seoul, Republic of Korea, 11-15th September, 2009
Non-Peer-Reviewed Publications
Suku. M., Forrester. L., Biggs. M, Monaghan, M. G, Tuning of Macrophage Polarization in the generation of functional cardiac organoids, EPSRC-SFI Joint Student Day, Online, 7th September, 2021
Shanley L., Mahon O. R., O'Rourke S. A., Neto N., Monaghan M. G., Kelly D., Dunne A, Particle size alters macrophage metabolism: Implications for bone tissue engineering, 34th Conference of the European Macrophage and Dendritic Cell Society, Online, 24-25th June , 2021
O'Rourke S., Neto N., Devilly E., Shanley L., Dunne A., Monaghan M. G, Inflammation in atherosclerosis: Investigating cholesterol crystals as potent drivers of metabolic reprogramming and M1 macrophage polarisation, Termis Workshop: Cell-Matrix Interaction and Mechanobiology in Regenerative Medicine, Online, 11-12th June, 2021
O'Rourke S., Neto N., Devilly E., Shanley L., Dunne A., Monaghan M. G, Immunometabolism in atherosclerosis: Investigating cholesterol crystals as potent drivers of metabolic reprogramming and M1 macrophage polarisation, 6th European Congress for Immunology Meeting, Online, 1-4th September , 2021
Fitzgerald H., Williams D. G., Campbell N. K., Barry P, O'Rourke S. A. Neto N., Monaghan M. G., Nolan D. P., Dunne A, Trypanosoma brucei secreted aromatic ketoacids activate the Nrf2/HO-1 pathway and suppress pro-inflammatory responses in primary murine and human immune cells, . 34th Conference of the European Macrophage and Dendritic Cell Society. Virtual conference , Online, 24-25th June, 2021
Shanley L., Mahon O. R., O'Rourke S. A., Neto N., Monaghan M. G., Kelly D., Dunne A, Particle size alters macrophage metabolism: Implications for bone tissue engineering, 6th European Congress for Immunology Meeting, Online, 1-4th September , 2021
O'Rourke S., Neto N., Devilly E., Shanley L., Dunne A., Monaghan M. G, ., Immunometabolism in atherosclerosis: Investigating cholesterol crystals as potent drivers of metabolic reprogramming and M1 macrophage polarisation, 34th Conference of the European Macrophage and Dendritic Cell Society, Online, 24-25th June , 2021
O'Rourke S., Neto N., Dunne A., Monaghan M. G., , Immune modulation in atherosclerosis: Investigating cholesterol crystals as potent drivers of metabolic reprogramming and M1 macrophage polarisation, Drexel Immune Modulation and Engineering Symposium, , Philadelphia, U.S.A, 11-13th November , 2020
Solazzo, M.*. Monaghan, M. G, Engineered electroconductive biomaterial platforms for cardiac tissue engineering applications, TBSI Research Symposium 2019, Dublin, 4th September , 2019
Neto N, O'Rourke S., Vishwanathan A., Dunne A., O'Farrelly C., Monaghan M. G., , Metabolic Profiling of Macrophage Polarization by Fluorescence Lifetime Imaging Microscopy, Immunometabolism Symposium, Dublin, 5th April , 2019
Michael Monaghan, Non-invasive, nonlinear microscopy in the evaluation of extracellular matrix and cell metabolic activity, ORS Musculoskeletal Summer Symposium, Dublin, 6-7th July, 2017, Dvid Hoey
Monaghan M, Browne S, Wang W, Pandit A, Scaffold-mediated non-viral inhibition of collagen type I and type III in cardiac fibroblasts, World Conference in Regenerative Medicine, Leipzig, Germany, 2-4th November, 2012
Monaghan M, Pandit A, Development of a 3D co-culture model to interpret the biological response of vascular biomaterials in vitro, , National Centre for Biomedical Engineering Science Research Day, Galway, Ireland, Galway, Ireland, March , 2008
Research Expertise
Description
The focus of the Monaghan lab (www.monaghanlab.com) is the development of novel biomaterial design-based strategies to regenerate or repair damaged tissues and restore biomechanical function using informed and optimised design approaches. Core Research Areas: . Cardiac tissue engineering and cell derivitisation from multi/pluripotent stem cell sources . Macrophage assessment and inclusion in advanced biomaterial culture platforms. . In silico predictive modelling and optimisation of biomaterial geometries . Electroconductive biomaterial synthesis, fabrication and applications . Advanced non-linear microscopy using multiphoton microscopy for non-invasive imaging of extracellular matrix . Advanced fluoresecent lifetime imaging microscopy (FLIM) in the assessment of cellular metabolism during disease, differentiation and regeneration. . Melt-electrowriting development, optimisation and application My lab is pioneering new approaches for biomaterial fabrication and design using advanced biomaterials, tissue engineering, biofabrication and in silico predictive modelling. My labs work will create a paradigm shift in which the scientific community, industry partners and clinical investigators approach biomaterial design. My lab is recognised internationally as world class where I am regularly invited to deliver keynote talks at major international meetings, and our papers have received awards for being most downloaded in our field. Members of my team have also been awarded prizes at national/international level in recognition of the scientific quality of our labs work. I have coordinated and elevated the Med3DP initiative over the past five years (www.med3dp.com) which develops medical devices for healthcare using 3D printing technology, demonstrating research led teaching and student led learning. My lab also has collaborative funding with small-medium enterprises such as Spraybase® (Bioprinting; next generation implantable devices) and I am a funded investigator in the SFI funded centres AMBER and CURAM. My work spans across and engages with several disciplines which includes Physics and Chemistry, Computer Science, Biochemistry and Immunology, Pharmacy, Surgery, Oncology and Endocrinology. This is strongly reflective in our illustrious repertoire of publications. Link to PubMed: http://www.ncbi.nlm.nih.gov/pubmed/?term=monaghan+AND+(pandit+OR+Walles+OR+schenke-layland) Link to Google Scholar: https://scholar.google.com/citations?user=X1mgIbEAAAAJ&hl=en&oi=sraProjects
- Title
- Enabling Melt ElectroWriting of Electroconductive, Geometrically Defined 3D Biomaterials to Promote the Maturation of Cardiomyocytes for Cardiac Regeneration
- Funding Agency
- Enterprise Ireland
- Date From
- December 2017
- Date To
- January 2020
- Title
- The role of macrophage polarization in the generation of functional engineered mature myocardium organoids
- Summary
- It is the purpose of this PhD project to intertwine the fields of bioengineering, biochemistry and immunology, by combining macrophages; key cells of the innate immune system, with cardiac organoids to achieve a more physiologically relevant model and achieve a diseased 'heart-attack-on-a-dish' organoid model as a patient specific pharmacological testing platform.
- Funding Agency
- Trinity College Dublin Faculty of Engineering Maths and Sciences Intrafaculty PhD College Award
- Date From
- September 2018
- Date To
- August 2022
- Title
- Cell-Specific Metabolic FLIM Profiling of the Ulcerative Colitis Microenvironment to Establish an Association with Patient Treatment Response
- Summary
- Non-invasive FLIM could reliably establish a statistically significant relationship between the FLIM characteristics of UC biopsies and UC patient response. The specific aims of this research project are to employ FLIM to characterise the microenvironment of lesions in UC. This includes measurement of the mean fluorescent decay times, contributing lifetimes and ratios of NADH and FAD within cellular populations dominating the UC microenvironment in a spatio-temporal manner. This will pave the way towards launching a FLIM imaging core facility that can reliably categorise cell specific metabolic profiles within tissue biopsies from patients diagnosed with UC, and provide recommendations for treatment regimens.
- Funding Agency
- Trinity College Dublin Provosts PhD Project Award
- Date From
- September 2018
- Date To
- August 2022
- Title
- Recapitulating electrical, mechanical and extracellular-matrix cues via multifunctional biomaterials to engineer cardiac tissue
- Funding Agency
- SFI-HRB-Wellcome Trust
- Date From
- January 2017
- Date To
- Septebmer 2019
- Title
- 3D Electroconductive biomaterial scaffolds as cardioinductive matrices for cardiac tissue engineering
- Summary
- Heart attacks lead to the death of functional cells in heart muscle (cardiomyocytes; CMs) which cannot be replaced. Every sixth man and seventh woman will die from a heart attack or related complications, and there is no way to regenerate this heart muscle. CMs in vivo are exposed to a unique environment defined by the local extracellular matrix (ECM), dynamic mechanical forces via the beating of the heart and electrical action potentials being generated by the heart's electrical conduction system. This proposal is a unique engineering approach to recapitulate a cardio-inductive platform based on mechanical, electrical and ECM cues to generate an efficient culture system to culture CMs in vitro and also to increase efficiencies of generating functional CMs from progenitor cells. This research aims to generate the following outcome: a cardio-inductive biomaterial system that has appropriate mechanical electroconductive and biocompatibility properties that can facilitate the generation of cardiac organoids in vitro using primary cells and efficiently facilitate cardiomyogenic differentiation from progenitor cells. These advances will not only improve quality of life for the patient but provide tools to study disease and perform pharmaceutical research.
- Funding Agency
- Irish Research Council
- Date From
- 01-SEP-19
- Date To
- 31-AUG-21
- Title
- Conductive materials for regeneration of the infarcted myocardium
- Summary
- Every sixth man and seventh woman will die from a heart attack or related complications Heart attacks lead to the death of functional cells in heart muscle (cardiomyocytes; CMs) which cannot be replaced. CMs in vivo are exposed to a unique environment defined by the local extracellular matrix (ECM), dynamic mechanical forces via the beating of the heart and electrical action potentials being generated by the heart's electrical conduction system. Electroconductive biomaterials in heart tissue engineering have the potential to be a building block for advanced therapeutic medical devices and also to achieve functional cardiac tissue in vitro. This project is a unique materials engineering approach to develop a cardio-inductive platform based on mechanical, electrical and ECM cues to generate the next generation of biomaterials. This research aims to generate the following outcome: a cardio-inductive biomaterial system derived from advanced nanomaterials that has appropriate mechanical, electroconductive and biocompatibility properties that can facilitate the generation of cardiac organoids in vitro using primary cells and efficiently facilitate cardiomyogenic differentiation from progenitor cells. These advances will not only improve quality of life for patients, but also provide tools to study disease and perform pharmaceutical research. This PhD program will develop a 3D biomaterial scaffold using synthetic nanomaterials together with naturally derived ECM that is biocompatible, suitably electroconductive and cardio-inductive towards facilitating the culture of cardiac organoids in vitro and enhancing stem cell cardiomyogenesis in vitro. Methods will include materials chemical characterisation techniques, cell culture, biochemical assays, mechanical testing, stem cell culture, immunohistochemistry, instrumentation. Support and training are provided throughout all stages.
- Funding Agency
- SFI
- Date From
- 01-Oct-19
- Date To
- 31-Sep-23
- Title
- Tuning macrophage polarization to model myocardial infarction in the generation of functional cardiac organoids'
- Summary
- It is the purpose of this PhD project to intertwine the fields of bioengineering, biochemistry and immunology, by combining macrophages; key cells of the innate immune system, with cardiac organoids to achieve a more physiologically relevant model and achieve a diseased 'heart-attack-on-a-dish' organoid model as a patient specific pharmacological testing platform. The generation of cardiac organoids can be achieved by recapitulating, as close as possible, the native microenvironment of the myocardium. Therefore, conductive polymers have gained interest as biomaterials for cardiac tissue engineering due to their suitable conductivity properties4. Very few reports have explored the interaction of macrophages and electroconductive biomaterials, and there are no reports regarding their introduction into cardiac organoids, or recapitulation of disease in a dish models
- Funding Agency
- EPSRC-SFI Joint Funding
- Date From
- 01-Oct-19
- Date To
- 31-Sep-23
- Title
- Research Boost Program
- Funding Agency
- TCD Dean of Research
- Date From
- November 2020
- Date To
- September 2021
- Title
- Enhancement of an Olympus FV1000MPE multiphoton microscope in Trinity Biomedical Sciences Institute (TBSI) for the application of Fluorescent Lifetime Imaging Microscopy (FLIM)
- Summary
- Enhancement of an Olympus FV1000MPE multiphoton microscope in Trinity Biomedical Sciences Institute (TBSI) for the application of Fluorescent Lifetime Imaging Microscopy (FLIM)
- Funding Agency
- SFI Funding internally allocated by TCD
- Date From
- 01-Feb-17
- Date To
- N/a
Recognition
Representations
Elected Council Member -Tissue Engineering and Regenerative Medicine International Society (EU Chapter)
Elected Treasurer- Matrix Biology Ireland 2017 Meeting
Reviewer for leading journals in the fields of Biomaterials and Tissue Engineering (selection only): Biomaterials, RSC Biomaterials Science, Advanced Optical Materials, ACS Biomaterials Science and Engineering, Macromolecular Materials and Engineering, Small, Macromolecular Bioscience, Advanced Functional Materials, Advanced NanoBiomed Research, Advanced Materials Technologies, Journal of Controlled Release, Molecular Therapy, Advanced Healthcare Materials, Tissue Engineering Part A, Tissue Engineering Part B, Acta Biomaterialia, ACS Applied Materials and Interfaces, Advanced Drug Delivery Reviews, Lab on a Chip, Nature Scientific Reports, Journal of Tissue Engineering and Regenerative Medicine, npj 2D Materials and Applications
External Examiner PhD Thesis Progress- Royal College of Surgeons in Ireland
Grant Reviewer for National and International Granting Bodies Including: European Research Council ADVANCED GRANTS European Research Council Starting Grants Wellcome Trust (2020-Present) MRC-AMED Regenerative Medicine and Stem Cell Research Initiative (2020- Present) Israel Science Foundation (2020-Present) Marie Sklowdowska-Curie Individual Fellowships (2019-Present) Freiburg Institute for Advanced Studies Deutsche Forschungsgemeinschaft (DFG) (2029-Present) Canada Foundation for Innovation (CFI) through the John R. Evans Leaders Fund (2021)
Awards and Honours
European Doctoral Award, Issued by the European Society of Biomaterials
Deutscher Akademischer Austausch Dienst, Short Term Travel Award to conduct research in University Hospital Tübingen
EMBO Short Term Travel Award to conduct research in Fraunhofer IGB Stuttgart
Roche Best Poster Award, NCBES Research day
NUI Galway College of Engineering and Informatics Research Fellow
University Scholar (Based on end of term results) NUI Galway
Memberships
Royal Academy of Medicine in Ireland (RAMI)- Bioengineering Section
MBI- Matrix Biology Ireland
ESB- European Society for Biomaterials
TERMIS - Tissue Engineering and Regenerative Medicine Society
DGMB-German Matrix Biology Society
Funded Investigator: SFI Funded Centre AMBER (Advanced Materials and BioEngineering Research)
Funded Investigator: SFI Funded Centre CÚRAM (Designing The Next Generation of 'Smart' Medical Devices)