Dr. Joanna Mcgouran

• Ying S, Chen Z, Medhurst AL, Neal JA, Bao Z, Mortusewicz O, McGouran J, Song X, Shen H, Hamdy FC, Kessler BM, Meek K, Helleday T, DNA-PKcs and PARP1 bind to unresected stalled DNA replication forks where they recruit XRCC1 to mediate repair., Cancer research, 2015Journal Article, 2015
• Watson AS, Riffelmacher T, Stranks A, Williams O, De Boer J, Cain K, MacFarlane M, McGouran J, Kessler B, Khandwala S, Chowdhury O, Puleston D, Phadwal K, Mortensen M, Ferguson D, Soilleux E, Woll P, Jacobsen SE, Simon AK, Autophagy limits proliferation and glycolytic metabolism in acute myeloid leukemia., Cell death discovery, 1, 2015Journal Article, 2015
• Bursomanno S, McGouran JF, Kessler BM, Hickson ID, Liu Y, Regulation of SUMO2 target proteins by the proteasome in human cells exposed to replication stress., Journal of proteome research, 14, (4), 2015, p1687-99Journal Article, 2015
• Bowles EJ, Schiffner T, Rosario M, Needham GA, Ramaswamy M, McGouran J, Kessler B, LaBranche C, McMichael AJ, Montefiori D, Sattentau QJ, Hanke T, Stewart-Jones GB, Comparison of neutralizing antibody responses elicited from highly diverse polyvalent heterotrimeric HIV-1 gp140 cocktail immunogens versus a monovalent counterpart in rhesus macaques., PloS one, 9, (12), 2014, pe114709Journal Article, 2014
• Maniam S, Coutts AS, Stratford MR, McGouran J, Kessler B, La Thangue NB, Cofactor Strap regulates oxidative phosphorylation and mitochondrial p53 activity through ATP synthase., Cell death and differentiation, 22, (1), 2015, p156-63Journal Article, 2015
• Blackledge NP, Farcas AM, Kondo T, King HW, McGouran JF, Hanssen LL, Ito S, Cooper S, Kondo K, Koseki Y, Ishikura T, Long HK, Sheahan TW, Brockdorff N, Kessler BM, Koseki H, Klose RJ, Variant PRC1 complex-dependent H2A ubiquitylation drives PRC2 recruitment and polycomb domain formation., Cell, 157, (6), 2014, p1445-59Journal Article, 2014
• Kaisaki PJ, Otto GW, McGouran JF, Toubal A, Argoud K, Waller-Evans H, Finlay C, Caldérari S, Bihoreau MT, Kessler BM, Gauguier D, Mott R, Genetic control of differential acetylation in diabetic rats., PloS one, 9, (4), 2014, pe94555Journal Article, 2014
• McGouran JF, Gaertner SR, Altun M, Kramer HB, Kessler BM, Deubiquitinating enzyme specificity for ubiquitin chain topology profiled by di-ubiquitin activity probes., Chemistry & biology, 20, (12), 2013, p1447-55Journal Article, 2013
• Zheng S, Moehlenbrink J, Lu YC, Zalmas LP, Sagum CA, Carr S, McGouran JF, Alexander L, Fedorov O, Munro S, Kessler B, Bedford MT, Yu Q, La Thangue NB, Arginine methylation-dependent reader-writer interplay governs growth control by E2F-1., Molecular cell, 52, (1), 2013, p37-51Journal Article, 2013
• Lercher L, McGouran JF, Kessler BM, Schofield CJ, Davis BG, DNA modification under mild conditions by Suzuki-Miyaura cross-coupling for the generation of functional probes., Angewandte Chemie (International ed. in English), 52, (40), 2013, p10553-8Journal Article, 2013
• Bogani D, Morgan MA, Nelson AC, Costello I, McGouran JF, Kessler BM, Robertson EJ, Bikoff EK, The PR/SET domain zinc finger protein Prdm4 regulates gene expression in embryonic stem cells but plays a nonessential role in the developing mouse embryo., Molecular and cellular biology, 33, (19), 2013, p3936-50Journal Article, 2013
• New M, Olzscha H, Liu G, Khan O, Stimson L, McGouran J, Kerr D, Coutts A, Kessler B, Middleton M, La Thangue NB, A regulatory circuit that involves HR23B and HDAC6 governs the biological response to HDAC inhibitors., Cell death and differentiation, 20, (10), 2013, p1306-16Journal Article, 2013
• Farcas AM, Blackledge NP, Sudbery I, Long HK, McGouran JF, Rose NR, Lee S, Sims D, Cerase A, Sheahan TW, Koseki H, Brockdorff N, Ponting CP, Kessler BM, Klose RJ, KDM2B links the Polycomb Repressive Complex 1 (PRC1) to recognition of CpG islands., eLife, 1, 2012, pe00205Journal Article, 2012
• McGouran JF, Kramer HB, Mackeen MM, di Gleria K, Altun M, Kessler BM, Fluorescence-based active site probes for profiling deubiquitinating enzymes., Organic & biomolecular chemistry, 10, (17), 2012, p3379-83Journal Article, 2012
• Cho EC, Zheng S, Munro S, Liu G, Carr SM, Moehlenbrink J, Lu YC, Stimson L, Khan O, Konietzny R, McGouran J, Coutts AS, Kessler B, Kerr DJ, Thangue NB, Arginine methylation controls growth regulation by E2F-1., The EMBO journal, 31, (7), 2012, p1785-97Journal Article, 2012
• Altun M, Kramer HB, Willems LI, McDermott JL, Leach CA, Goldenberg SJ, Kumar KG, Konietzny R, Fischer R, Kogan E, Mackeen MM, McGouran J, Khoronenkova SV, Parsons JL, Dianov GL, Nicholson B, Kessler BM, Activity-based chemical proteomics accelerates inhibitor development for deubiquitylating enzymes., Chemistry & biology, 18, (11), 2011, p1401-12Journal Article, 2011
• Boutureira O, McGouran JF, Stafford RL, Emmerson DP, Davis BG, Accessible sugars as asymmetric olefin epoxidation organocatalysts: glucosaminide ketones in the synthesis of terminal epoxides., Organic & biomolecular chemistry, 7, (20), 2009, p4285-8Journal Article, 2009
• Bush J., Walport L., McGouran J., Leung I., Berridge G., Van Berkel S., Basak A., Kessler B., Schofield C., The Ugi four-component reaction enables expedient synthesis and comparison of photoaffinity probes, Chemical Science, 4, (11), 2013, p4115-4120Journal Article, 2013, DOI
• Ghari F, Quirke AM, Munro S, Kawalkowska J, Picaud S, McGouran J, Subramanian V, Muth A, Williams R, Kessler B, Thompson PR, Fillipakopoulos P, Knapp S, Venables PJ, La Thangue NB, Citrullination-acetylation interplay guides E2F-1 activity during the inflammatory response., Science advances, 2, (2), 2016, pe1501257Journal Article, 2016, DOI
• Grijzenhout A, Godwin J, Koseki H, Gdula M, Szumska D, McGouran JF, Bhattacharya S, Kessler BM, Brockdorff N, Cooper S, Functional analysis of AEBP2, a PRC2 Polycomb protein, reveals a Trithorax phenotype in embryonic development and in ES cells., Development (Cambridge, England), 2016Journal Article, 2016, DOI
• Doherty W, Adler N, Knox A, Nolan D, McGouran J, Nikalje A.P, Lokwani D, Sarkate A, Evans P, Synthesis and Evaluation of 1,2,3-Triazole-Containing Vinyl and Allyl Sulfones as Anti-Trypanosomal Agents, European Journal of Organic Chemistry, 2017, (1), 2017, p175 - 185Journal Article, 2017, DOI , URL , TARA - Full Text
• Biochemical and mass spectrometry-based approaches to profile sumoylation in human cells in, Activity-Based Proteomics, Humana Press, New York, NY, Springer, 2017, pp131-144 , [Kessler, B.M., Bursomanno, S., McGouran, J.F., Hickson, I.D., Liu, Y.]Book Chapter, 2017
• Raza, F., McGouran, J.F., Kessler, B.M., Possee, R.D., King, L.A., Phosphorylation induces structural changes in the Autographa californica nucleopolyhedrovirus P10 protein, Journal of Virology, 91, (13), 2017Journal Article, 2017
• Abdullah, U.B., Mcgouran, J.F., Brolih, S., Ptchelkine, D., El-Sagheer, A.H., Brown, T., Mchugh, P.J., RPA activates the XPF-ERCC1 endonuclease to initiate processing of DNA interstrand crosslinks, EMBO Journal, 2017Journal Article, 2017
• Mills, E.L. and Ryan, D.G. and Prag, H.A. and Dikovskaya, D. and Menon, D. and Zaslona, Z. and Jedrychowski, M.P. and Costa, A.S.H. and Higgins, M. and Hams, E. and Szpyt, J. and Runtsch, M.C. and King, M.S. and McGouran, J.F. and Fischer, R. and Kessler, B.M. and McGettrick, A.F. and Hughes, M.M. and Carroll, R.G. and Booty, L.M. and Knatko, E.V. and Meakin, P.J. and Ashford, M.L.J. and Modis, L.K. and Brunori, G. and Sévin, D.C. and Fallon, P.G. and Caldwell, S.T. and Kunji, E.R.S. and Chouchani, E.T. and Frezza, C. and Dinkova-Kostova, A.T. and Hartley, R.C. and Murphy, M.P. and O'Neill, L.A., Itaconate is an anti-inflammatory metabolite that activates Nrf2 via alkylation of KEAP1, Nature, 556, (7699), 2018, p113-117Journal Article, 2018, DOI , URL
• Turnbull AP, Ioannidis S, Krajewski WW, Pinto-Fernandez A, Heride C, Martin ACL, Tonkin LM, Townsend EC, Buker SM, Lancia DR, Caravella JA, Toms AV, Charlton TM, Lahdenranta J, Wilker E, Follows BC, Evans NJ, Stead L, Alli C, Zarayskiy VV, Talbot AC, Buckmelter AJ, Wang M, McKinnon CL, Saab F, McGouran JF, Century H, Gersch M, Pittman MS, Marshall CG, Raynham TM, Simcox M, Stewart LMD, McLoughlin SB, Escobedo JA, Bair KW, Dinsmore CJ, Hammonds TR, Kim S, Urbé S, Clague MJ, Kessler BM, Komander D., Molecular basis of USP7 inhibition by selective small-molecule inhibitors., Nature, 550, (7677), 2017, p481-486Journal Article, 2017, DOI , TARA - Full Text
• Unsworth, A.J., Bombik, I., Pinto-Fernandez, A., McGouran, J.F., Konietzny, R., Zahedi, R.P., Watson, S.P., Kessler, B.M., Pears, C.J., Human Platelet Protein Ubiquitylation and Changes following GPVI Activation, Thrombosis and Haemostasis, 119, (1), 2019, p104-116Journal Article, 2019
• Dürr, E.-M., Doherty, W., Lee, S.Y., El-Sagheer, A.H., Shivalingam, A., McHugh, P.J., Brown, T., McGouran, J.F., Squaramide-Based 5"-Phosphate Replacements Bind to the DNA Repair Exonuclease SNM1A, ChemistrySelect, 3, (45), 2018, p12824-12829Journal Article, 2018, TARA - Full Text
• Doherty W., Durr E.-M., Baddock H.T., Lee S.Y., McHugh P.J., Brown T., Senge M.O., Scanlan E.M., McGouran J.F., A hydroxamic-acid-containing nucleoside inhibits DNA repair nuclease SNM1A, Organic and Biomolecular Chemistry, 17, (35), 2019, p8094 - 8105Journal Article, 2019, DOI , TARA - Full Text
• Howells A., McGouran J.F., Kessler B.M., Luhn K., Dengue virus stimulation of dendritic cells induces phosphorylation- and proteolysis-dependent signalling, Malaysian Journal of Microbiology, 14, (Specialissue6), 2018, p474 - 482Journal Article, 2018, DOI
• Berney M., McGouran J.F., Methods for detection of cytosine and thymine modifications in DNA, Nature Reviews Chemistry, 2, (11), 2018, p332 - 348Journal Article, 2018, DOI , TARA - Full Text
• Thomas A.M., Brolih S., McGouran J.F., El-Sagheer A.H., Ptchelkine D., Jones M., McDonald N.Q., McHugh P.J., Brown T., Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors, Chemical Communications, 55, (78), 2019, p11671 - 11674Journal Article, 2019, DOI , TARA - Full Text
• Taylor, N.C. Hessman, G. Kramer, H.B. McGouran, J.F., Probing enzymatic activity - a radical approach, Chemical Sciences, (11), 2020, p2967 - 2972Journal Article, 2020
• Joanna McGouran, Holger Kramer & Benedikt Kessler, 'Active Site probes', Isis Innovation Limited, WO 2013/144656 Al, 2013Patent, 2013, URL
• Taylor, N.C. McGouran, J.F., Strategies to Target Specific Components of the Ubiquitin Conjugation/Deconjugation Machinery, Frontiers in Chemistry, 7, (914), 2020, p1-9Journal Article, 2020, TARA - Full Text
• Berney, M. and Manoj, M.T. and Fay, E.M. and McGouran, J.F., 5†-Phosphorylation Increases the Efficacy of Nucleoside Inhibitors of the DNA Repair Enzyme SNM1A, ChemMedChem, 17, (5), 2022Journal Article, 2022, DOI
• McKenna, S.M. and Fay, E.M. and McGouran, J.F., Flipping the Switch: Innovations in Inducible Probes for Protein Profiling, ACS Chemical Biology, 16, (12), 2021, p2719-2730Journal Article, 2021, DOI
• Berney, M. and Doherty, W. and Jauslin, W.T. and T Manoj, M. and DÃŒrr, E.-M. and McGouran, J.F., Synthesis and evaluation of squaramide and thiosquaramide inhibitors of the DNA repair enzyme SNM1A, Bioorganic and Medicinal Chemistry, 46, (116369), 2021Journal Article, 2021, DOI
• Taylor, N.C. and McGouran, J.F., Investigating eosin Y as a photocatalyst for the radical-dependent activity-based probing of deubiquitinating enzymes, Organic and Biomolecular Chemistry, 19, (10), 2021, p2177-2181Journal Article, 2021, DOI
• DÃŒrr, E.-M. and McGouran, J.F., Probing the binding requirements of modified nucleosides with the dna nuclease snm1a, Molecules, 26, (2), 2021Journal Article, 2021, DOI
• Ghari, F. and Quirke, A.-M. and Munro, S. and Kawalkowska, J. and Picaud, S. and McGouran, J. and Subramanian, V. and Muth, A. and Williams, R. and Kessler, B. and Thompson, P.R. and Fillipakopoulos, P. and Knapp, S. and Venables, P.J. and Thangue, N.B.L., Immunology: Citrullination-acetylation interplay guides E2F-1 activity during the inflammatory response, Science Advances, 2, (2), 2016Journal Article, 2016, DOI , URL
• Calvert, S.H. and McGouran, J.F., Rapid capture of small peptide binders, Nature Reviews Chemistry, 7, (8), 2023, p534-535Journal Article, 2023, DOI , URL
• Fay, E.M. and Newton, A. and Berney, M. and El-Sagheer, A.H. and Brown, T. and McGouran, J.F., Two-Step Validation Approach for Tools To Study the DNA Repair Enzyme SNM1A, ChemBioChem, 24, (13), 2023Journal Article, 2023, DOI , URL
• Arbour, C.A. and Fay, E.M. and McGouran, J.F. and Imperiali, B., Deploying solid-phase synthesis to access thymine-containing nucleoside analogs that inhibit DNA repair nuclease SNM1A, Organic and Biomolecular Chemistry, 21, (28), 2023, p5873-5879Journal Article, 2023, DOI , URL
• Fay, E.M. and McGouran, J.F., Exploring the binding pockets of the DNA damage repair enzyme SNM1A through nucleobase modification, Results in Chemistry, 5, (100939), 2023Journal Article, 2023, DOI , URL
• McKenna, S.M. and Florea, B.I. and Zisterer, D.M. and van Kasteren, S.I. and McGouran, J.F., Probing the metalloproteome: an 8-mercaptoquinoline motif enriches minichromosome maintenance complex components as significant metalloprotein targets in live cells, RSC Chemical Biology, 5, (8), 2024, p776-786Journal Article, 2024, DOI , URL
• Calvert, S.H. and Pawlak, T. and Hessman, G. and McGouran, J.F., Rapid diazotransfer for selective lysine labelling, Organic and Biomolecular Chemistry, 2024Journal Article, 2024, DOI , URL

• Joanna McGouran, Activatable probes targeting deubiquitinating enzymes, Activity-Based Protein Profiling 2019, Leuven, Belgium, 27th March, 2019, Prof. Martin Witte & Prof. Steven VerhelstInvited Talk
• Joanna McGouran, Latent probes for the covalent capture of active enzymes, RSC Ireland Regional Meeting , UCD, Ireland, 7th June, 2019, Prof. Pat GuiryInvited Talk
• Joanna McGouran, 5' oligonucleotide modification for enhanced nuclease binding, Young Investigators Workshop, Merton College, Oxford, UK, 23-25th August, 2018, Prof. Veronique GrouvernerInvited Talk
• Joanna McGouran, Chemical proteomics: Protein modification to unravel biological function, RSC Organic Symposium , TCD, Ireland, 18th May, 2018, Prof. Eoin ScanlanInvited Talk
• Joanna McGouran, DNA modification to examine 5' exonuclease activity, The 18th RSC Chemical Biology and Bioorganic Group (CBBG) Firbush conference, Edinburgh, UK, 6-8th September, 2019Oral Presentation
• Joanna McGouran, Proteins and DNA-New Tools through Chemical Modification, Research seminar, Nuffield Department of Medicine, University of Oxford, Target Discovery Institute, 28/02, 2020, Dr Nicola TernetteInvited Talk
• Joanna McGouran & Neil Taylor, 'Protein Labelling', Barker Brettell LLP, 2020, 1 November 2022Patent

• Title

  Activity based probes to examine DNA damage repair function and activity in cell systems
  Summary
  Funding Agency

  Wellcome Trust ISSF/Trinity College Dublin
  Date From

  04/2017
  Date To

  04/2019
• Title

  Oligonucleotide probes to study the 5' exonuclease SNM1A and its function in DNA damage repair
  Summary
  Funding Agency

  Irish research Council
  Date From

  10/2017
  Date To

  10/2021
• Title

  New Probes for active enzymes
  Summary
  Funding Agency

  Trinity College Dublin
  Date From

  01/2020
  Date To

  01/2024
• Title

  A New Approach to Inhibiting Nucleotide Excision Repair Using Cyclic Peptides
  Summary
  Funding Agency

  Irish research Council
  Date From

  10/2019
  Date To

  10/2023
• Title

  New Probes for deubiquitinating enzymes
  Summary
  Funding Agency

  Trinity College Dublin
  Date From

  09/2016
  Date To

  09/2020
• Title

  Activity based probes for DNA processing enzymes based on modified Cytosine
  Summary
  Funding Agency

  Irish research Council
  Date From

  10/2019
  Date To

  10/2023
• Title

  Probing DNA damage repair
  Summary
  Funding Agency

  Trinity College Dublin
  Date From

  09/2016
  Date To

  09/2020
• Title

  Activity Based Probes to reveal new insights into P53 deubiquitination
  Summary
  Funding Agency

  Irish research Council
  Date From

  09/2020
  Date To

  09/2024
• Title

  Disulfide modification for enhanced stability and selective delivery
  Summary
  Funding Agency

  Science Foundation Ireland
  Date From

  09/2020
  Date To

  09/2024

Biochemistry and cell biology, Chemical Sciences,

• Scholarship, University of Oxford 2002-5
• MA University of Oxford 2009

• American Chemical Society Present
• Visiting assistant professor, University College Dublin 2026
• Royal Society of Chemistry Present
• The Irish Mass Spectrometry Society Present

• Swiss National Foundation evaluation committee of the SNSF special call on coronaviruses 2020
• Member of the Royal Society of Chemistry Chemical Biology and Bioorganic Group Committee. 2021
• External Examiner, PhD thesis: University of Oxford, University of Glasgow. Masters thesis: University College Dublin. 2019
• Reviewer for several international chemistry journals including publications from the American Chemical Society, Royal Society of Chemistry, Wiley, Elsevier, MDPI and Sage 2019
• Reviewer for UKRI grants including EPSRC and MRC 2020
• Member of the Early Career Advisory Board for the American Chemical Society's new journal ACS Bio & Med Chem Au 2022