Sequencing

 

TrinSeq, the Trinity Genome Sequencing Laboratory is located in the Trinity Translational Medicine Institute (TTMI), Trinity College Dublin. The laboratory now houses an Illumina MiSeq, a benchtop next generation sequencing platform that can undertake a range of ultra-high throughput genomics applications. This is a core facility supported by full-time research staff available to all academic and industry-based researchers in Ireland.

June 2009 press release : Minister Lenihan Opens Ireland’s First ‘Next-Generation’ DNA Laboratory at TCD’s Institute of Molecular Medicine

 

MiSeq Technology

This ultra high-throughput sequencing system enables a wide range of genetic analysis applications including de novo sequencing of novel genomes, resequencing of whole genomes or target regions (e.g. candidate disease genes), transcriptome profiling and the study of DNA regulatory mechanisms. The MiSeq system is powered by Illumina sequencing technology, which uses a massively parallel sequencing-by-synthesis approach to generate billions of bases of high-quality DNA sequence per run.

 

 

Applications

DNA Sequencing	Transcriptome analysis	Gene Regulation and Control
Whole genome resequencing Targeted or amplicon resequencing De novo sequencing 16S Metagenomics Clone Checking / Plasmid Sequencing	Relative expression of transcripts Analysis of splice variants/coding SNPs Analysis of non-coding RNAs Transcript discovery Small RNA sequencing	Genome-wide analysis of Epigenetic modifications DNA-protein interactions (ChIP-seq)

Core Facility

The Trinity Genome Sequencing Laboratory provides the following assistance to researchers interested in undertaking a project using the Illumina MiSeq Benchtop Sequencer:

• Advice on study design and costs
• Advice and guidance on sample library preparation
• Access to automated liquid handling for large scale projects
• Sequencing and primary data analysis
• Advice and guidance on secondary data analysis

How To Get Started

For online information on how to set up your MiSeq sample prep and sequence run: http://www.illumina.com/informatics/research/experimental-design/basespace-prep.html

For more information on MiSeq reagent kits:
http://support.illumina.com/sequencing/sequencing_kits/miseq_reagent_kit.ilmn

For a list of publications using the Illumina Sequencing Technology go to:

For project enquiries/project quotes please contact: Dr Elaine Kenny

 

 

The Trinity Genome Sequencing Laboratory was established with funding from Science Foundation Ireland through a grant awarded to Dr. Derek Morris.