Richard Porter
Associate Professor, Biochemistry

Biography

Dr. Porter did his undergraduate degree in Biochemistry at Trinity College. This was followed by a Ph.D with Prof. John M. Scott and Dr. Martin Brand in the area of carbon-one metabolism and a three year postdoctoral position at the Department of Biochemistry University of Cambridge UK. Dr. Porter's research focuses on understanding mammalian energy metabolism. His research achievements are based ideas founded in solid science, a substantial grant base, the quality of the researchers that he recruits, the advanced technology in the laboratory and his collaborations with prominent international laboratories, both academic and industrial. Dr. Porter has made several discoveries in the following areas of mammalian energy metabolism: (a) the molecular basis of basal metabolism in mammals, (b) discovery of a novel mitochondrial transporters related to folic acid metabolism, (c) the characterization of energy dysfunction in the brain of aged mammals, (d) mitochondrial function in immune cells and (e) his recent contributions to the elucidation of the mechanism of UCP function. His research has biotechnological relevance in that it has implications (both immediate and long-term) for anti-obesity, anti-type II and immunomodulatory strategies, with recent advances including a potential drug target for anti-obesity/anti-type 2 diabetes strategies.

Publications and Further Research Outputs

Peer-Reviewed Publications

Martin DS, Leonard S, Devine R, Redondo C, Kinsella GK, Breen CJ, McEneaney V, Rooney MF, Munsey TS, Porter RK, Sivaprasadarao A, Stephens JC, Findlay JB., Novel mitochondrial complex I inhibitors restore glucose-handling abilities of high-fat fed mice., J Mol Endocrinol , 56, (3), 2016, p261 - 271 Journal Article, 2016 DOI

Clarke, K.J., Carroll, A.M., O'Brien, G., Porter, R.K. , Detection of UCP1 protein and measurements of dependent GDP-sensitive proton leak in non- phosphorylating thymus mitochondria, Methods in Molecular Biology, 1241, 2015, p123-135 Journal Article, 2015 DOI

Lynam-Lennon N, Connaughton R, Carr E, Mongan AM, O'Farrell NJ, Porter RK, Brennan L, Pidgeon GP, Lysaght J, Reynolds JV, O'Sullivan J, Excess visceral adiposity induces alterations in mitochondrial function and energy metabolism in esophageal adenocarcinoma., BMC cancer, 14, 2014, p907 Journal Article, 2014 TARA - Full Text DOI

Breen EP, Pilgrim W, Clarke KJ, Yssel C, Farrell M, Zhou J, Murphy PV, Porter RK. , Lack of activation of UCP1 in isolated brown adipose tissue mitochondria by glucose-O-ω-modified saturated fatty acids of various chain lengths., Journal of Chemical Biology, 6, (3), 2013, p121 - 133 Journal Article, 2013 URL TARA - Full Text

Clarke, KJ, Porter, RK, Uncoupling protein 1 dependent reactive oxygen species production by thymus mitochondria, INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 45, (1), 2013, p81-89 Journal Article, 2013 DOI

Orlagh M. Kelly, Yvonne M. McNamara, Lars H. Manzke, Mary J. Meegan, Richard K. Porter, The Preservation of in vivo Phosphorylated and Activated Uncoupling Protein 3 (UCP3) in Isolated Skeletal Muscle Mitochondria following Administration of 3,4-Methylenedioxymethamphetamine (MDMA aka Ecstasy) to Rats/Mice, Mitochondrion, 12, (1), 2012, p110-9 Journal Article, 2012 TARA - Full Text

Porter, R.K., Studies on the function and regulation of mitochondrial uncoupling proteins, Advances in Experimental Medicine and Biology, 748, 2012, p171 - 184 Journal Article, 2012

Clarke, KJ, Adams, AE, Manzke, LH, Pearson, TW, Borchers, CH, Porter, RK, A role for ubiquitinylation and the cytosolic proteasome in turnover of mitochondrial uncoupling protein 1 (UCP1), BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1817, (10), 2012, p1759-1767 Journal Article, 2012 DOI

Orlagh M. Kelly, Richard K. Porter, Absence of mitochondrial uncoupling protein 3: Effect on thymus and spleen in the fed and fasted mice, Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1807, (9), 2011, p1064-1074 Journal Article, 2011 TARA - Full Text

Andrea Dlaskovà, Kieran J. Clarke, Richard K. Porter, R.K. , The role of UCP 1 in the production of reactive oxygen species by mitochondria isolated from brown adipose tissue, Biochimica Biophysica Acta (Bioenergetics), 1797, (8), 2010, p1470 - 1476 Journal Article, 2010 TARA - Full Text

Alison E. Adams, Orlagh M. Kelly, Richard K.Porter, R. K., Absence of Mitochondrial Uncoupling Protein 1 Affects Apoptosis in Thymocytes, Thymocyte/T-Cell profile and Peripheral T-Cell Number, Biochimica et Biophysica Acta (BBA) - Bioenergetics, 16th European Bioenergetics Conference, Warsaw, 17-22 July, edited by L. Wojtczak and K. Zabłocki , 1797, (6-7), Elsevier, 2010, pp807-816 Conference Paper, 2010 TARA - Full Text

Niaobh O'Donoghue, Trevor Sweeney, Robin Donagh, Kieran Clarke and Richard K. Porter , Control of Choline Oxidation in Rat Kidney Mitochondria, Biochimica Biophysica Acta (Bioenergetics), 1787, 2009, p1135 - 1139 Journal Article, 2009 TARA - Full Text

Murphy, James E.J. and Porter, Richard K. , The Control of Oxidative Phosphorylation in the Adrenal Gland Cell Line (Y1), Advances in Experimental Medicine and Biology, 645, 2009, p35 - 41 Journal Article, 2009

Porter R.K. & Fallon P.G., mitochondrial UCP 1 and thymus function, EBEC 2008 Short Reports, EBEC 2008, Trinity College Dublin, 19-24 July 2008, edited by Richard K. Porter & Gavin Davey , 1777, 2008, ppS34- Meeting Abstract, 2008

Adams AE, Carroll AM, Fallon PG, Porter RK., Mitochondrial uncoupling protein 1 expression in thymocytes., Biochimica et Biophysica Acta (BBA) - Bioenergetics , 1777, (7-8), 2008, p772 - 776 Journal Article, 2008 DOI TARA - Full Text

Davey G. & Porter Richard K., Bioenergetics: Going from Strength to strength, Biochimica Biophysica Acta (Bioenergetics), EBEC 2008, Trinity College Dublin, 19-24 July 2008, edited by Richard K. Porter & Gavin Davey , 1777, (7-8), Elsevier, 2008, pp549 - 549 Meeting Abstract, 2008

Dlaskova, A. & Porter R.K., regulation of H2O2 generation by uncoupling protein UCP 1 in BAT and thymus mitochondria, EBEC 2008 Short reports, EBEC 2008, Trinity College Dublin, 19-24 July 2008, edited by Richard K. Porter & Gavin Davey , 1777, Elsevier, 2008, ppS65- Meeting Abstract, 2008

Adams, Alison E., Carroll, Audrey M., Fallon, Padraic G. & Porter, Richard K., Mitochondrial uncoupling protein 1 expression in thymocytes, Biochimica Biophysica Acta (Bioenergetics), 1777, (7-8), 2008, p772 - 775 Journal Article, 2008

Clarke K.J. & Porter R.K. , The effect of preserving covalent modification of mitochondria by phosphorylation on oxygen consumption by mitochondria isolated from brown adipose tissue and thymus, EBEC 2008 Short Reports, EBEC 2008, Trinity College Dublin, 19-24 July 2008, edited by Richard K. Porter & Gavin Davey , 1777, Elsevier, 2008, ppS54- Meeting Abstract, 2008

Alison E. Adams, Orla Hanrahan, Derek N. Nolan, H. Paul Voorheis, Padraic Fallon and Richard K. Porter, Images of mitochondrial UCP 1 in mouse thymocytes using confocal microscopy , Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1777, (2), 2008, p115 - 117 Journal Article, 2008 TARA - Full Text DOI

Porter Richard K., Preface, EBEC Short reports, EBEC 2008, Trinity College Dublin, 19-24 July 2008, edited by Richard K. Porter & Gavin Davey , 1777, Elsevier, 2008, ppS1- Meeting Abstract, 2008

Adams A.E. Porter R.K. , Apoptosis in thymocytes from UCP 1 wild-type and UCP 1 knock-out mice, EBEC 2008 Short reports, EBEC 2008, Trinity College Dublin, 19-24July 2008, edited by Richard K. Porter & Gavin Davey , 1777, 2008, ppS44- Meeting Abstract, 2008

Porter Richard K., uncoupling protein 1: A short-circuit in the Chemiosmotic process, the Journal of Bioenergetics and Biomembranes, 40, 2008, p457 - 461 Journal Article, 2008

Carroll, Audrey M., Porter, Richard K. & Morrice, Nicolas A., Identification of serine phosphorylation in mitochondrial uncoupling protein 1, Biochimica Biophysica Acta (Bioenergetics), 1777, (7-8), 2008, p1060 - 1065 Journal Article, 2008

Beck V, Jabùrek M, Demina T, Rupprecht A, Porter RK, Jezek P, Pohl EE., Polyunsaturated fatty acids activate human uncoupling proteins 1 and 2 in planar lipid bilayers., FASEB J, 21, (4), 2007, p1137-1144 Journal Article, 2007

Adams, A.E., Hanrahan, O., Nolan, D.N., Voorheis, H.P. Fallon, P. and Porter, R.K., Images of Mitochondrial UCP 1 in Mouse Thymocytes using Confocal Microscopy, Biochimica Biophysica Acta (Bioenergetics), 1777, 2007, p115 - 117 Journal Article, 2007

Beck, V., Jabùrek, M., Breen, E.P., Porter, R.K., Je ek, P. and Pohl, E.E. , A new automated technique for the reconstitution of hydrophobic proteins into planar bilayer membranes. Studies of human recombinant uncoupling protein 1., Biochimica Biophysica Acta (Bioenergetics), 1757, 2006, p474 - 479 Journal Article, 2006

Brennan, C.M., Breen, E.P. and Porter, R.K., Cold acclimation and oxygen consumption in the thymus, Biochimica Biophysica Acta (Bioenergetics), 1757, (11), 2006, p1463-1468 Journal Article, 2006

Porter, R.K., A New Look at Mitochondrial Uncoupling Protein 1, Biochimica Biophysica Acta, 1757, 2006, p446 - 448 Journal Article, 2006

Brennan, C.M. & Porter R.K., The effects of acute and endurance exercise on UCP 3 protein expression in mouse gastrocnemius, plantaris, soleus and EDL muscles, EBEC Short Reports, EBEC 2006, Moscow, July, edited by V. Borisov , 14, Elsevier/Biochimica Biophysica Acta (Bioenergetics), 2006, pp363- Meeting Abstract, 2006

Breen, E.P., Gouin, S., Brennan, C.M., Murphy, A.F., Haines, L.R., Pearson, T.W., Murphy, P.V. and Porter, R.K., On the mechanism of mitochondrial uncoupling protein 1 function, Journal of Biological Chemistry, 281, 2006, p2114 - 2119 Journal Article, 2006

Carroll, A.M., Haines, L.R., Pearson, T.W., Fallon, P.G., Walsh, C.M., Brennan, C., Breen, E. & Porter, R.K., Identification of a functioning mitochondrial uncoupling protein 1 in thymus, Journal of Biological Chemistry, 280, 2005, p155534 - 155543 Journal Article, 2005 TARA - Full Text DOI

Carroll, A.M., Haines, L.R., Pearson, T.W., Fallon, P., Walsh, C., Brennan, C., Breen, E.P. & Porter, R.K., Detection of a functioning UCP 1in Thymus, Journal of Biological Chemistry, 280, 2005, p15534 - 15543 Journal Article, 2005

Gouin, S.G., Breen, E.P., Pilgrim, W., Murphy, P.V., Porter R.K., Elucidation of the mechanism of mitochondrial uncoupling protein function using a synthetic glycolipid, Mitochondria in Physiology, MiP2005, 2005 Journal Article, 2005 URL

Gouin, S.G., Breen, E.P., Pilgrim, W., Murphy, P.V. & Porter R.K., Elucidation of the mechanism of mitochondrial uncoupling protein using a synthetic glycolipid, Bioscience 2005, Glasgow, Scotland. UK, 2005 Meeting Abstract, 2005 URL

Gouin, S.G, Pilgrim, W., Porter, R.K. and Murphy, P.V., Synthesis of a glycolipid for studying mechanisms of mitochondrial uncoupling proteins, Carbohydrate Research, 340, 2005, p1547 - 1552 Journal Article, 2005 URL URL

Carroll, A.M., Haines, L.R., Pearson, T.W., Brennan, C., Breen, E.P. & Porter R.K., Immunodetection of UCP 1 in Rat Thymus, Biochemical Society Transactions, 32, (6), 2004, p1066 - 1067 Journal Article, 2004 URL

Carroll, A.M., Haines, L.R., Pearson, T.W., Brennan, C., Breen, E. & Porter, R.K., Identification of a functioning uncoupling protein 1 in rat thymus, EBEC Short Report, 13, 2004, pp203 Conference Paper, 2004

Carroll, A.M. & Porter, R.K., Starvation sensitive UCP 3 expression in rat thymus and spleen, Biochim. Biophys. Acta, 1700, 2004, p145 - 150 Journal Article, 2004 URL

Cunningham, O., McElligott, A.M., Carroll, A.M., Breen, E., Reguenga, C., Oliveira, M.E.M, Azevedo, J.E. & Porter, R.K., Selective Detection of UCP 3 Expression in Skeletal Muscle: Effect of Thyroid Status and Temperature Acclimation, Biochim. Biophys. Acta, 1604, 2003, p170 - 179 Journal Article, 2003 URL

Joyce, O.J.P., Farmer, M.K. Tipton, K.F. & Porter, R.K., Oxidative Phosphorylation by in situ Synaptosomal Mitochondria from Whole Brain of Young and Old Rats, J. Neurochem., 86, 2003, p1032 - 1041 Journal Article, 2003 URL

Brennan, C.M, McElligott, A.M. Warmington, S.A. & Porter, R.K., Skeletal muscle uncoupling protein-3 expression following endurance swimming training in mice, Proceedings of the Journal of Physiology, 543, 2002, pp119 Conference Paper, 2002

Porter, R.K., Allometry of mammalian cellular oxygen consumption, Cellular and Molecular Life Sciences, 58, 2001, p815 - 822 Journal Article, 2001 URL

Porter, R.K., Mitochondrial Proton Leak: A Role for Uncoupling Proteins 2 & 3 ?, Biochim. Biophys. Acta, 1504, (1), 2001, p120 - 127 Journal Article, 2001 URL

Porter, R.K., Mammalian mitochondria; inner membrane cationic and neutral amino acid carriers, Biochim. Biophys. Acta, 1459, 2000, p356 - 362 Journal Article, 2000 URL

Melia, H.P. Andrews, J.F., McBennett, S.M. & Porter, R.K., Leptin and Efficiency of Oxidative Phosphorylation, FEBS Letts., 458, 1999, p261 - 264 Journal Article, 1999 URL

Porter, R.K., Joyce, O.J.P., Farmer, M.K., Heneghan, R., Tipton, K.F, Andrews, J. F., McBennett, S.M., Lund, M.D., Jensen, C.H. & Melia, H.P, Indirect measurement of mitochondrial proton leak and its application, International Journal of Obesity, 23, 1999, pS12 - S18 Journal Article, 1999 URL

Porter, R.K. & Andrews, J.F., Effects of leptin on mitochondrial 'proton leak' and uncoupling proteins: implications for mammalian energy metabolism, Proceedings of the Nutrition Society, 57, 1998, p455 - 460 Journal Article, 1998 URL

Porter, R.K., Hulbert, A.J. and Brand, M.D., Allometry of Mitochondrial Proton Leak: Influence of Membrane Surface Area and Fatty Acid Composition, The American Journal of Physiology, 271, 1996, pR1550 - R1560 Journal Article, 1996 URL

Porter, R.K. & Brand M.D., Cellular oxygen consumption depends on body mass, American Journal of Physiology, 269, (1 part 2), 1995, pR226 - R228 Journal Article, 1995 URL

Porter, R.K. and Brand, M.D., Causes of the Differences in Respiration Rate of Hepatocytes from Mammals of Different Body Mass, The American Journal of Physiology, 269, 1995, pR1213 - R1224 Journal Article, 1995 URL

Porter, R.K. and Brand, M.D., Mitochondrial Proton Conductance and H+/O Ratio are Independent of Electron Transport Rate in Isolated Hepatocytes, Biochemical Journal, 310, 1995, p379 - 382 Journal Article, 1995 URL

Brand, M.D., Chien, L.-F., Ainscow, E.K., Rolfe, D.F.S. & Porter, R.K., Causes and Functions of Mitochondrial Proton Leak, Biochimica Biophysica Acta, 1187, 1994, p132 - 139 Journal Article, 1994 URL

R. K. Porter, J. M. Scott and M. D. Brand, Characterization of betaine efflux from rat liver mitochondria, Biochimica et biophysica acta, 1141, (02-Mar), 1993, p269-74 Journal Article, 1993 URL

Porter, R.K. and Brand, M.D., Body Mass Dependence of Proton Leak in Mitochondria and its Relevance to Metabolic Rate, Nature, 362, 1993, p628 - 630 Journal Article, 1993 URL

Kaplan, C.P., Porter, R.K. and Brand, M.D., The Choline Transporter is the Major Site of Control of Choline Oxidation in Isolated Rat Liver Mitochondria, FEBS Letters, 321, 1993, p24 - 26 Journal Article, 1993 URL

R. K. Porter, J. M. Scott and M. D. Brand, The nature of betaine efflux from rat liver mitochondria, Biochemical Society transactions, 20, (3), 1992, p247S Journal Article, 1992 URL

R. K. Porter, J. M. Scott and M. D. Brand, Choline transport into rat liver mitochondria. Characterization and kinetics of a specific transporter, The Journal of biological chemistry, 267, (21), 1992, p14637-46 Journal Article, 1992 URL

R. K. Porter, J. M. Scott and M. D. Brand, Choline transport into rat liver mitochondria, Biochemical Society transactions, 20, (3), 1992, p248S Journal Article, 1992 URL

Non-Peer-Reviewed Publications

Brennan, C.M. McElligott, A.M., Warmington, S.A. and Porter, R.K., Skeletal muscle uncoupling protein-3 expression following endurance swimming training in mice, Proceedings of the Physiological Society, The Journal of Physiology, University of Liverpool, July, 543, (P), 2002, pp119p Meeting Abstract, 2002 URL

Cadenas, S., Jekabsons, M.B., Carroll, A.M., Porter, R.K. & Brand, M.D., Rat muscle mitochondria treated with Dowex are GDP insensitive, 674th Biochemical Society Meeting, 29, 2001, pp123 Meeting Abstract, 2001

Maguire, D.J., Joyce, O. & Porter, R.K., Mitoprotein separation on 2-D Native Blue Gels; Miniaturization and resolution of extraction artefacts, Human Genetics Society of Australasia Meeting, 2001 Meeting Abstract, 2001

Cunningham, O, Carroll, A.M. & Porter R.K., RT-PCR evidence for the expression of UCP-2 in spleen and murine macrophage cell line (J774), 674th Biochemical Society Meeting, 29, 2001, pp119 Meeting Abstract, 2001

Brennan, C.M., McElligott, A.M., Warmington, S.A. and Porter, R.K., Skeletal muscle uncoupling protein 3 expression following endurance swimming training in mice, Proceedings of the 674th Biochemical Society Meeting, Trinity College Dublin, July, 2001 Meeting Abstract, 2001

Breen, E.P. & Porter, R.K., Discriminatory antibody raised to UCP-3 used to detect protein in a number of systems, 674th Biochemical Society Meeting, 29, 2001, pp120 Meeting Abstract, 2001

Porter, R.K. and Brand, M.D., Mitochondrial Properties Affecting Proton Leak Kinetics in Mammals of Different Body Mass, 646th Biochemical Society Meeting, 1993 Meeting Abstract, 1993

Brennan, C.M., McElligott, A.M., Warmington, S.A. & Porter, R.K., Skeletal muscle uncoupling protein 3 expression following endurance swimming training in mice, 674th Biochemical Society Meeting, 29, pp120 Meeting Abstract,

Research Expertise

Description

Bioenergetics of Immune cell Function. Anti-Obesity and anti-type 2 diabetes strategies. Senescence and mitochondrial dysfunction. Energy Metabolism. Efficiency of energy transduction in mammals. Mitochondrial function. Mitochondrial Uncoupling proteins. Mitochondrial Transporters.Metabolic control/elasticity analysis.

Projects

  • Title
    • Mitochondrial Proteome
  • Summary
    • Investigation and identification of novel mitochondrial transporters/ transporter regulators. [Euros 1.2M] Collaborators: Keith Tipton, Gavin Davey and Caroline Jefferies
  • Funding Agency
    • Marie Curie
  • Date From
    • January 2007
  • Date To
    • January 2009
  • Title
    • The mechanism of function mitochondriual uncoupling proteins
  • Funding Agency
    • Science Foundation Ireland (SFI)
  • Date From
    • January 2007
  • Date To
    • September 2010
  • Title
    • The role of Mitochondrial Efficiency in Thymus Function
  • Funding Agency
    • The Health Research Board of Ireland
  • Date From
    • 1 October 2008
  • Date To
    • 30 september 2011
  • Title
    • An examination of the biochemical control of cellular energetic efficiency in muscle of beef cattle divergently selected for residual feed intake (RFI)
  • Summary
    • (i) To establish and physiologically characterise two populations of cattle, genetically and phenotypically divergent in energetic efficiency (based on residual feed intake - RFI) (ii) To examine changes in temporal gene expression in muscle tissue of cattle divergent in energetic efficiency (iii) To examine the premise that efficiency of energy transduction in cattle is a function of mitochondrial abundance and mitochondrial functional efficiency. (iv) To examine the expression of cellular proteins differentially expressed between energetically efficient and inefficient animals will be examined using targeted and global approaches. (v) To: i) conduct a bioinformatical analysis of the mitochondrial data (Task 2) transcription profiles (Task 3) and proteomic data (Task 4); ii) integrate physiological, mitochondrial, transcriptional and proteomic data important to the regulation of energetic efficiency and iii) identify potential biomarkers for selection of cattle which have a superior ability for muscle energetic efficiency.
  • Funding Agency
    • Teagasc
  • Date From
    • 2011
  • Date To
    • 2014

Keywords

(HUMAN); (RAT LIVER); (SKELETAL MUSCLE); Ageing, memory and other cognitive processes; Analytical Techniques; Basic Skills Education; Biochemistry, Carbohydrates; Biochemistry, Lipids; Biochemistry, metabolism; Biochemistry, Proteins; BODY-MASS; BRAIN; BROWN-ADIPOSE-TISSUE; Diabetes & Obesity; Diabetes and diabetic complications; Drug discovery; Energy Chemical Sciences; Immune system; Innate immunology; Life Sciences; Lipids, steroids, membranes; Membrane Transport; Membrane transport, structure and dynamics; Metabolic Biology; Metabolism and metabolic diseases; Mitochondria; Neuroscience; Photosynthesis; Proteins, enzymology and protein engineering; Thermal Effects

Recognition

Representations

Chairman of European Bioenergetics Conference 2008 (EBEC 2008)[www.tcd.ie/Biochemistry/ebec2008/][ebec2008@tcd.ie] 2008

Scientific advisor to Iperboreal Pharma Pescara Italy

Awards and Honours

Lucker-Cobbe Award

Fellow of Trinity College Dublin 2007

Memberships

Treasurer of the Irish Area Section of the Biochemical Society (IASBS) 2009 – 2012

Member of The European Bioenergetics Conference Committee 2004

Member of the Biochemical Society 1986 – Present

Member of the Irish Area Section of the Biochemical Society 2006

Member of the International Society for Oxygen Transport in Tissues (ISOTT) 2007 – 2008

Appointed to the Bioenergetics and Metabolism Theme Panel III of the Biochemical Society. 2004 – 2007

Editor of Elsevier's Biochimica Biophysica Acta (Bioenergetics) 2008