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Conor Buckley
Assistant Professor, Mechanical & Manuf. Eng

Biography

Buckley graduated with a degree in Mechanical Engineering (2001) and completed a PhD in the area of scaffolds and bone tissue engineering (2007). In 2011, he was appointed as Assistant Professor in Biomedical Engineering (Biomaterials and Tissue Engineering) at Trinity College Dublin. He is a Principal investigator (PI) in the Trinity Centre for Bioengineering and a funded Investigator (FI) in the Advanced Materials and Bioengineering Research (AMBER) Centre at Trinity College Dublin. His research focuses on developing novel biomaterials and cell based strategies for tissue regeneration using minimally invasive technologies (http://www.mee.tcd.ie/regenerative/People/CBuckley). He is also the Director of Orthobiologics and Adjunct Assistant Prof in the Academic unit of Orthopaedics and Sports Medicine and Course Director of the award winning MSc in Bioengineering. Buckley founded Med3DP (www.med3dp.com), an initiative to develop medical devices for humanitarian healthcare using 3D printing technology. Buckley is a co-founding member and treasurer of European Society- Educating Students in Engineering and Medicine (ESEM) and also serves as a board member for Common European Masters in Biomedical Engineering (CEMACUBE). In 2014 he was awarded an IRC New Foundations Scholarship and was a visiting Professor in the Department of Orthopaedics, at Mount Sinai, New York, USA. He has served as reviewer for 22 leading journals in the fields of Biomaterials and Tissue Engineering and is an editorial board member of Frontiers- Bioengineering and Biotechnology. He was a co-chair of the 19th Annual Conference of the Section of Bioengineering of the Royal Academy of Medicine in Ireland, Dublin, Ireland, Jan 2013 and has also served as an external grant reviewer for European agencies. He is also a scientific ambassador, education and outreach mentor for the Trinity Science Gallery which involves showcasing of research and mentoring secondary level students interested in pursuing a degree/career in engineering and science. Buckley has previously mentored 1 postdoctoral fellow (injectable biomaterials for minimally invasive repair of the intervertebral disc), supervised 4 PhD and 20 M.Sc. (Bioengineering). His current research group consists of 2 Postdoctoral Fellows, 3 Ph.D. and 2 MSc students in the area of Biomaterials & Tissue Engineering. Prof Buckley has been awarded a number of grants in the area of tissue engineering and regenerative medicine as Principal Investigator (>1.26million) and as co-investigator (>3.45million) and has published 60 international peer reviewed journal articles and over 150 conference publications (h index= 25, citations=1716, May 2017). Most recently he received a Career Development Award (Intraoperative Single Stage Procedure for Intervertebral Disc Regeneration (INSPIRE)) from Science Foundation Ireland.

Publications and Further Research Outputs

Peer-Reviewed Publications

Vedicherla, S. and Buckley, C.T., In vitro extracellular matrix accumulation of nasal and articular chondrocytes for intervertebral disc repair, Tissue and Cell, 49, (4), 2017, p503-513 Journal Article, 2017 TARA - Full Text DOI

Gansau J. and Buckley C.T., Injectable cell-loaded microcapsules as a potential strategy for nucleus pulposus regeneration., BioSpine 2017- 6th International Congress on Biotechnologies for Spinal Surgery, Langenbeck-Virchow-Haus, Berlin, Germany, 2017 Meeting Abstract, 2017

Almeida H.V., Sathy B.N., Dudurych I., Buckley C.T., O'Brien F.J. and Kelly D.J. , Shape-Memory Alginate Scaffolds Functionalized with either Type I or Type II Collagen for Cartilage Tissue Engineering, Tissue Engineering , 23, (1-2), 2017, p55 - 68 Journal Article, 2017

Vedicherla S.V., Withers D., Moran C.J. and Buckley C.T. , Enhanced Integration using Freshly Isolated Human Infrapatellar Fat Pad Cell and Chondrocyte Coculture with a Polyurethane Meniscal Replacement, 63rd Annual Meeting of the Orthopaedic Research Society, San Diego Convention Center, California, USA, 2017 Meeting Abstract, 2017

Gilroy, D.A. and Hobbs, C. and Nicolosi, V. and Buckley, C.T. and O'Brien, F.J. and Kearney, C.J., Development of magnetically active scaffolds as intrinsically-deformable bioreactors, MRS Communications, 7, (3), 2017, p367-374 Journal Article, 2017 DOI

Reina Rodriguez F.S., Buckley C.T. and Kirby B.M., Biomechanical studies of feline ventral abdominal wall and celiotomy closure techniques., Veterinary Surgery, 2017, p1-11 Journal Article, 2017 DOI

Vedicherla S.V. and Buckley C.T. , In Vitro Extracellular Matrix Accumulation of Nasal and Articular Chondrocytes for Intervertebral Disc Repair, Tissue and Cell , 49, (4), 2017, p503-513 Journal Article, 2017 TARA - Full Text DOI

Carroll, S.F., Buckley, C.T. and Kelly, D.J., Cyclic tensile strain can play a role in directing both intramembranous and endochondral ossification of mesenchymal stem cells., Frontiers in Bioengineering and Biotechnology, section Tissue Engineering and Regenerative Medicine. , 2017 Journal Article, 2017

Srujana Vedicherla, Conor Timothy Buckley, Rapid Chondrocyte Isolation for Tissue Engineering Applications: The Effect of Enzyme Concentration and Temporal Exposure on the Matrix Forming Capacity of Nasal Derived Chondrocytes, BioMed Research International, 2017, 2017, p2395138- Journal Article, 2017 DOI TARA - Full Text

Browe D., Mahon O., Dudurych I., Dunne A., Buckley C.T. and Kelly D.J., The Combination of Rapidly Isolated Infrapatellar Fat Pad Stromal Cells and Glyoxal Cross-linked Type II Collagen Scaffolds As a Single-stage Therapy For Articular Cartilage Repair, 63rd Annual Meeting of the Orthopaedic Research Society, San Diego Convention Center, California, USA, 2017 Meeting Abstract, 2017

Carroll S.F., Sathy B.N., Buckley C.T. and Kelly D.J. , Cyclic Tensile Strain Directs Both Intramembranous And Endochondral Ossification Of MSCs, 63rd Annual Meeting of the Orthopaedic Research Society, San Diego Convention Center, California, USA, 2017 Meeting Abstract, 2017

Vedicherla S.V., Romanazzo S., Kelly D.J., Buckley C.T.* and Moran C.J.* , Chondrocyte based Intraoperative Processing Strategies for the Biological Augmentation of a Polyurethane Meniscal Replacement, Connective Tissue Research , 2017 Journal Article, 2017

Vedicherla, S., Buckley, C.T., Cell-based therapies for intervertebral disc and cartilage regeneration- Current concepts, parallels, and perspectives, Journal of Orthopaedic Research, 35, (1), 2017, p8 - 22 Review Article, 2017 TARA - Full Text DOI

Gansau J. and Buckley C.T. , Effect of fibrin concentration on matrix deposition capacity of articular chondrocytes for nucleus pulposus regeneration, Tissue Engineering and Regenerative Medicine International Society EU (TERMIS-EU), Uppsala, Sweden, 2016 Meeting Abstract, 2016

Carroll S.F., Sathy B.N., Buckley C.T. and Kelly D.J. , Cyclic tensile strain enhances both intramembranous and endochondral ossification of MSCs encapsulated within fibrin hydrogels, Tissue Engineering and Regenerative Medicine International Society EU (TERMIS-EU), Uppsala, Sweden, 2016 Meeting Abstract, 2016

Naqvi, S.M. and Buckley, C.T. , Bone Marrow Stem Cells in Response to Intervertebral Disc-Like Matrix Acidity and Oxygen Concentration - Implications for Cell-Based Regenerative Therapy, Spine, 41, (9), 2016, p743 - 750 Journal Article, 2016 TARA - Full Text

Carroll S.F., Sathy B.N., Buckley C.T. and Kelly D.J. , Mechanical cues and cell shape regulate the myogenic versus chondrogenic fate decision of MSCs, Tissue Engineering and Regenerative Medicine International Society EU (TERMIS-EU), Uppsala, Sweden, 2016 Meeting Abstract, 2016

Mesallati T., Buckley C.T. and Kelly D.J. , Engineering cartilaginous grafts using chondrocyte-laden hydrogels supported by a superficial layer of stem cells, Journal of Tissue Engineering and Regenerative Medicine , 5, (18), 2016, p2353-2362 Journal Article, 2016 DOI

Almeida, H.V., Eswaramoorthy, R., Cunniffe, G.M., Buckley, C.T., O'Brien, F.J., Kelly, D.J., Fibrin hydrogels functionalized with cartilage extracellular matrix and incorporating freshly isolated stromal cells as an injectable for cartilage regeneration, Acta Biomaterialia, 36, 2016, p55-62 Journal Article, 2016 DOI

Naqvi S.M., Vedicherla S.V., Gansau J, McIntyre T, Doherty M and Buckley C.T., Living Cell Factories- Electrosprayed Microcapsules and Microcarriers for Minimally Invasive Delivery, Advanced Materials , 28, (27), 2016, p5662-5671- Journal Article, 2016 TARA - Full Text

Luo, L., O'Reilly, A.R., Thorpe, S.D., Buckley, C.T., Kelly, D.J., Engineering zonal cartilaginous tissue by modulating oxygen levels and mechanical cues through the depth of infrapatellar fat pad stem cell laden hydrogels, Journal of Tissue Engineering and Regenerative Medicine, 11, (9), 2016, p2613-2628 Journal Article, 2016 DOI

Browe D.C., Dudurych I., Buckley C.T. and Kelly D.J. , Combining glyoxal cross-linked type II collagen based scaffolds with infrapatellar fat pad stromal cells for articular cartilage repair, Tissue Engineering and Regenerative Medicine International Society EU (TERMIS-EU), Uppsala, Sweden, 2016 Meeting Abstract, 2016

Naqvi, S.M. and Buckley, C.T. , Extracellular matrix production by nucleus pulposus and bone marrow stem cells in response to altered oxygen and glucose microenvironments, Journal of Anatomy, 227 , 2015, p757 - 766 Journal Article, 2015 TARA - Full Text

Almeida, A.V, Cunniffe, G.M., Vinardell, T., Buckley, C.T., O'Brien, F.J., Kelly, D.J. , Coupling freshly isolated CD44+ infrapatellar fat pad derived stromal cells with a tgf-β3 eluting cartilage ecm-derived scaffold as a single stage strategy for promoting chondrogenesis. , Advanced Healthcare Materials, 4, (7), 2015, 1043-1053 Journal Article, 2015 DOI

Vedicherla S.V. and Buckley C.T, Tissue forming capacity of nasal and articular derived chondrocytes for intervertebral disc regeneration- Effect of oxygen and TGFβ stimulation, 4th Tissue Engineering and Regenerative Medicine International Society (TERMIS) World Congress, Boston, USA., 2015 Meeting Abstract, 2015

Naqvi, S.M., Buckley, C.T., Differential response of encapsulated nucleus pulposus and bone marrow stem cells in isolation and coculture in alginate and chitosan hydrogels, Tissue Engineering - Part A, 21, (1-2), 2015, p288-299 Journal Article, 2015 TARA - Full Text

Gilroy, D.A., O'Brien F.J., Buckley C.T. and Kearney C.J. , Magnetically active biomaterials for stem cell activation, UK Society for Biomaterials, University of Ulster, Northern Ireland, 2015 Meeting Abstract, 2015

Luo L., Thorpe S.D., Buckley C.T. and Kelly D.J. , The effects of dynamic compression on the development of cartilage grafts engineered using bone marrow and infrapatellar fat pad derived stem cells, Biomedical Materials, 21, (10(5)), 2015, p055011 Journal Article, 2015 DOI

Guillaume, O., Naqvi, S.M., Lennon, K. and Buckley C.T. , Enhancing cell migration in shape-memory alginate-collagen composite scaffolds: in vitro and ex vivo assessment for intervertebral disc repair, Journal of Biomaterials Applications, 29, (9), 2015, p1230 - 1246 Journal Article, 2015 TARA - Full Text

Naqvi S.M. and Buckley C.T. , Bone Marrow-Derived Stem Cells in Response to Intervertebral Disc-Like Matrix Acidity - Implications for Cell-Based Regenerative Therapy, 4th Tissue Engineering and Regenerative Medicine International Society (TERMIS) World Congress, Boston, USA., 2015 Meeting Abstract, 2015

Mesallati T, Sheehy E.J, Vinardell T, Buckley C.T, Kelly D.J, Tissue engineering scaled-up, anatomically shaped osteochondral constructs for joint resurfacing, European Cells and Materials, 30, 2015, p163 - 186 Journal Article, 2015 URL TARA - Full Text URL

Aisa, J., Calvo, I., Buckley, C.T., Kirby, B.M., Mechanical Comparison of Loop and Crimp Configurations for Extracapsular Stabilization of the Cranial Cruciate Ligament-Deficient Stifle, Veterinary Surgery, 44, (1), 2015, p50-58 Journal Article, 2015

Naqvi S.M. and Buckley C.T. , Effect of Priming and Structured Co-culture of Microencapsulated Bone Marrow and Adipose Tissue Derived Stem Cells for Disc Repair, 61st Annual Meeting of the Orthopaedic Research Society, MGM Grand Hotel in Las Vegas, Nevada, USA, 2015 Meeting Abstract, 2015

Sridharan R, Reilly RB, Buckley CT, Decellularized grafts with axially aligned channels for peripheral nerve regeneration., Journal of the mechanical behavior of biomedical materials, 41, 2015, p124 - 135 Journal Article, 2015 DOI TARA - Full Text

Sheehy E.J., Mesallati T., Vinardell T., Kelly L., Buckley C.T. and Kelly D.J., Tissue engineering whole bones through endochondral ossification: Regenerating the distal phalanx, BioResearch, 4, (1), 2015, p229 - 241 Journal Article, 2015 TARA - Full Text DOI

Buckley C.T. and Vedicherla S.V. , Towards a Single Stage Procedure for Intervertebral Disc Repair using Nasal Septal Chondrocytes, ORS PSRS 3rd International Spine Research Symposium, Philadelphia, USA, 2015 Oral Presentation, 2015

3D Printing and Additive Manufacturing for Biomedical Engineering in, editor(s)K. Kelly and G. O'Donnell , Perspectives on Manufacturing- Essays in honour of John Monaghan., 2015, pp85 - 96, [Buckley C.T. ] Book Chapter, 2015

Sanders, R., Kearney, C, Buckley, C.T., Jenner, F. and Brama, P. , Knot security of 5 metric (USP 2) sutures: influence of knotting technique, suture material, and incubation time for 14 and 28 days in phosphate buffered saline and inflamed equine peritoneal fluid, Veterinary Surgery, 44, (6), 2015, p723 - 730 Journal Article, 2015

Naqvi S.M. and Buckley C.T. , Cryopreservation of Primed Microencapsulated Bone Marrow Derived Stem Cells Using Electrohydrodynamic Spraying For On Demand Minimally Invasive Tissue Regeneration of the Intervertebral Disc, 4th Tissue Engineering and Regenerative Medicine International Society (TERMIS) World Congress, Boston, USA., 2015 Meeting Abstract, 2015

Almeida H.V, Cunniffe G.M, Vinardell T, Buckley C.T, O'Brien F.J, Kelly D.J, Coupling Freshly Isolated CD44<sup>+</sup> Infrapatellar Fat Pad-Derived Stromal Cells with a TGF-β3 Eluting Cartilage ECM-Derived Scaffold as a Single-Stage Strategy for Promoting Chondrogenesis, Advanced Healthcare Materials, 4, (7), 2015, p1043 - 1053 Journal Article, 2015 DOI URL

Kelly, L.J., Guillaume O., Naqvi S.M., Robinson A.J. and Buckley C.T., Cell Microencapsulation Using Electrohydrodynamic Spraying for Minimally Invasive Tissue Repair, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Genova, Italy, 2014 Meeting Abstract, 2014

Baboolal T.G., Boxall S.A., Churchman S.M., Buckley C.T., Jones E. and McGonagle D. , Intrinsic multipotential mesenchymal stromal cell activity in gelatinous Heberden's nodes in osteoarthritis at clinical presentation., Arthritis Research & Therapy , 16, (3), 2014, pR19 Journal Article, 2014 TARA - Full Text

Guillaume, O., Gansau, J., Lennon, K., Quinn, S.J., Kaye-Coyle, R. and Buckley C.T. , Fabrication of Porous Scaffolds Derived from Decellularized Annulus Fibrosus Extracellular Matrix, 60th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, USA, 2014 Meeting Abstract, 2014

Naqvi S.M. and Buckley C.T. , Response of Bone Marrow-Derived Stem Cells Cultured in Intervertebral Disc-Like Microenvironmental Conditions, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Genova, Italy, 2014 Meeting Abstract, 2014

Guillaume, O., Daly, A., Lennon K., Gansau J., Buckley S.F. and Buckley, C.T., Shape-memory porous alginate scaffolds for regeneration of the annulus fibrosus - Effect of TGF-β3 supplementation and oxygen culture conditions, Acta Biomaterialia, 10, 2014, p1985 - 1991 Journal Article, 2014 TARA - Full Text

Sheehy E.J., Mesallati T., Buckley C.T. and Kelly D.J. , Anatomic Hypertrophic Cartilaginous Grafts For Whole Bone Tissue Engineering, 60th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, USA, 2014 Meeting Abstract, 2014

Mesallati,Tariq T., Buckley,Conor Timothy C.T., Kelly,Daniel John D.J., Engineering articular cartilage-like grafts by self-assembly of infrapatellar fat pad-derived stem cells, Biotechnology and Bioengineering, 111, (8), 2014, p1686-1698 Journal Article, 2014 DOI

Liu Y, Buckley CT, Almeida HV, Mulhall KJ, Kelly DJ, Infrapatellar Fat Pad-Derived Stem Cells Maintain Their Chondrogenic Capacity in Disease and Can be Used to Engineer Cartilaginous Grafts of Clinically Relevant Dimensions., Tissue engineering. Part A, 20, (21-22), 2014, p3050-62 Journal Article, 2014 TARA - Full Text DOI

Kelly, L.J., Naqvi S.M. and Buckley C.T. , Microencapsulation of bone marrow derived stem cells using electrohydrodynamic spraying for minimally invasive tissue repair of the IVD, eCM XV: Cartilage & Disc: Repair and Regeneration, Davos, Switzerland, 2014 Meeting Abstract, 2014

Sridharan, R., Reilly, R.B. and Buckley C.T. , Decellularized Grafts with Longitudinal Oriented Channels for Peripheral Nerve Regeneration, 60th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, USA, 2014 Meeting Abstract, 2014

Sheehy EJ, Vinardell T, Toner ME, Buckley CT, Kelly DJ, Altering the architecture of tissue engineered hypertrophic cartilaginous grafts facilitates vascularisation and accelerates mineralisation., PloS one, 9, (3), 2014, pe90716 Journal Article, 2014 TARA - Full Text DOI

Mesallati T, Buckley CT, Kelly DJ, A comparison of self-assembly and hydrogel encapsulation as a means to engineer functional cartilaginous grafts using culture expanded chondrocytes., Tissue engineering. Part C, Methods, 20, (1), 2014, p52-63 Journal Article, 2014 TARA - Full Text DOI

Carroll, S.F., Buckley, C.T., Kelly, D.J., Cyclic Hydrostatic Pressure Promotes A Stable Cartilage Phenotype And Enhances The Functional Development Of Cartilaginous Grafts Engineered Using Multipotent Stromal Cells Isolated From Bone Marrow And Infrapatellar Fat Pad. , Journal of Biomechanics, 47, (9), 2014, p2115 - 2121 Journal Article, 2014 TARA - Full Text

Mesallati T., Sheehy E.J., Vinardell T., Buckley C.T. and Kelly D.J., Tissue Engineering Scaled-Up, Anatomically Accurate, Osteochondral Constructs for Joint Resurfacing, 60th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, USA, 2014 Meeting Abstract, 2014

Naqvi, S.M., Guillaume, O., and Buckley C.T, Oxygen is a More Potent Regulator than Glucose Concentration for Matrix Production of Nucleus Pulposus and Bone Marrow-derived Stem Cells, 60th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, USA, 2014 Meeting Abstract, 2014

Kearney C.M., Buckley C.T., Jenner F and Brama P.A.J. , Elasticity and Breaking Strength of Synthetic Suture Materials Incubated in Various Equine Physiological and Pathological Solutions, Equine Veterinary Journal , 46, (4), 2014, p494 - 498 Journal Article, 2014

Almeida H.V., Liu, Y. Cunniffe G.M., Mulhall K.J., Matsiko A., Buckley C.T., O'Brien F.J. and Kelly D.J. , Controlled release of TGF-β3 from cartilage extra cellular matrix derived scaffolds to promote chondrogenesis of human joint tissue derived stem cells, Acta Biomaterialia , 10, (10), 2014, p4400 - 4409 Journal Article, 2014

Almeida H.V., Liu, Y. Cunniffe G.M., Buckley C.T., O'Brien F.J. and Kelly D.J. , The Composition and Architecture of Cartilage ECM Derived Scaffolds Regulates Stem Cell Infiltration and their Chondrogenic Differentiation, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Istanbul, Turkey, 2013 Meeting Abstract, 2013

O'heireamhoin S, Buckley CT, Jones E, McGonagle D, Mulhall KJ, Kelly DJ, Recapitulating Aspects of the Oxygen and Substrate Environment of the Damaged Joint Milieu for Stem Cell-Based Cartilage Tissue Engineering., Tissue engineering. Part C, Methods, 19, (2), 2013, p117-127 Journal Article, 2013 DOI TARA - Full Text

Luo L., Thorpe S.D., Buckley C.T. and Kelly D.J., Engineering zonal cartilaginous tissue using infrapatellar fat pad derived stem cells by modulating the spatial environment within the developing construct, Annual Meeting of the Orthopaedic Research Society, San Antonio, Texas, USA , 2013 Meeting Abstract, 2013

Guillaume, O., Daly, A., Naqvi, S.M. and Buckley, C.T. , Development of Shape-Memory Porous Scaffolds for Minimal Invasive Repair of the Annulus Fibrosus, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Istanbul, Turkey, 2013 Meeting Abstract, 2013

Carroll, S.F., Buckley, C.T. and Kelly, D.J. , The influence of hydrostatic pressure on the functional development & phenotypic stability of cartilaginous grafts engineered using mesenchymal stem cells isolated from bone marrow & infrapatellar fat pad, Annual Meeting of the Orthopaedic Research Society, San Antonio, Texas, USA, 2013 Meeting Abstract, 2013

Sheehy EJ, Vinardell T, Buckley CT, Kelly DJ, Engineering osteochondral constructs through spatial regulation of endochondral ossification., Acta biomaterialia, 9, (3), 2013, p5484-5492 Journal Article, 2013 DOI TARA - Full Text

Maguire S.C., Naqvi S.M., Tryfon M.E. and Buckley C.T. , Co-Culture of Nucleus Pulposus and Mesenchymal Stem Cells in a Nutrient Limited In Vitro System- Implications for Intervertebral Disc Regeneration Strategies, Annual Meeting of the Orthopaedic Research Society, San Antonio, Texas, USA, 2013 Meeting Abstract, 2013

Liu, Y., Buckley, C.T., Mulhall, K.J., Kelly, D.J., Combining BMP-6, TGF-β3 and hydrostatic pressure stimulation enhances the functional development of cartilage tissues engineered using human infrapatellar fat pad derived stem cells, Biomaterials Science, 1, (7), 2013, p745-752 Journal Article, 2013 DOI TARA - Full Text

Sheehy E.J., Vinardell T., Buckley C.T. and Kelly D.J. , Towards Engineering Whole Bones via Endochondral Ossification, ASME 2013 Summer Bioengineering Conference, Oregon, USA, 2013 Meeting Abstract, 2013

Naqvi, S.M., Tryfon, M.K. and Buckley, C.T. , Determining Optimal Cell Ratios of Nucleus Pulposus and Mesenchymal Stem Cells Co-Cultured in a Nutrient Deprived Microenvironment, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Istanbul, Turkey, 2013 Meeting Abstract, 2013

Mesallati, T., Buckley, C.T., Nagel, T., Kelly, D.J., Scaffold architecture determines chondrocyte response to externally applied dynamic compression. , Biomechanics and Modeling in Mechanobiology, 12, (5), 2013, p889-899 Journal Article, 2013 DOI

Haugh, M.G., Thorpe, S.D., Vinardell, T., Duffy, G.P. and Kelly, D.J. , The application of plastic compression to modulate fibrin hydrogel mechanical properties., Journal of the Mechanical Behavior of Biomedical Materials, 16, 2012, p66-72 Journal Article, 2012 DOI

Liu Y., Mesallati T., Buckley C.T., Downey R., Mulhall K. and Kelly D.J. , Scaffold-free cartilaginous tissue engineered using osteoarthritic human infrapatellar fat pad derived stem cells, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Hofburg Congress Centre, Vienna, Austria, 2012 Meeting Abstract, 2012

Vinardell T, Sheehy EJ, Buckley CT, Kelly DJ, A comparison of the functionality and in vivo phenotypic stability of cartilaginous tissues engineered from different stem cell sources., Tissue engineering. Part A, 18, (11-12), 2012, p1161-1170 Journal Article, 2012 DOI

Sheehy E.J., Vinardell T., Buckley C.T. and Kelly D.J. , Engineering Osteochondral Constructs through Spatial Regulation of Endochondral Ossification, Annual Meeting of the Orthopaedic Research Society, San Francisco, California, USA, 2012 Meeting Abstract, 2012

O'Eireamhoin S. *, Buckley C.T. *, Schepens A, Jones E, McGonagle D, Mulhall K.J. and Kelly D.J., The effects of growth factor supplementation and oxygen tension during expansion on subsequent chondrogenesis of human infrapatellar fat pad derived mesenchymal stem cells, Journal of Bone & Joint Surgery, British Volume 94 (SUPP XXXIX), 2012 Published Abstract, 2012

Cox G., Boxhall S., Giannoudis P.V., Buckley C.T., Roshdy T., Churchman S.M., McGonagle D. and Jones E.: , High abundance of CD271+ multipotential stromal cells (MSCs) in intramedullary cavities of long-bones, Bone, 50, (2), 2012, p510-517 Journal Article, 2012 TARA - Full Text

Liu Y., Buckley C.T., Downey R., Mulhall K. and Kelly D.J., Chemical and physical cues to regulate the development of cartilaginous tissues engineered using osteoarthritic human infrapatellar fat pad derived stem cells, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Hofburg Congress Centre, Vienna, Austria, 2012 Meeting Abstract, 2012

Mesallati T., Buckley C.T. and Kelly D.J. , Engineering functional cartilaginous grafts using chondrocyte agarose constructs supported by a superficial layer of stem cells, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Hofburg Congress Centre, Vienna, Austria, 2012 Meeting Abstract, 2012

Thorpe S.D., Buckley C.T., Steward A.J. and Kelly D.J. , Extrinsic biophysical stimulus can override the influence of local substrate in determining stem cell fate, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Hofburg Congress Centre, Vienna, Austria, 2012 Meeting Abstract, 2012

Liu Y, Buckley CT, Downey R, Mulhall KJ, Kelly DJ, The role of environmental factors in regulating the development of cartilaginous grafts engineered using osteoarthritic human infrapatellar fat pad-derived stem cells., Tissue engineering. Part A, 18, (15-16), 2012, p1531-1541 Journal Article, 2012 DOI TARA - Full Text

Sheehy EJ, Buckley CT, Kelly DJ, Oxygen tension regulates the osteogenic, chondrogenic and endochondral phenotype of bone marrow derived mesenchymal stem cells., Biochemical and biophysical research communications, 417, (1), 2012, p305-310 Journal Article, 2012 DOI

Carroll, S.F., Buckley, C.T. and Kelly, D.J. , Oxygen consumption during chondrogenesis in hydrogels seeded with chondrocytes and stem cells isolated from different tissues, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Hofburg Congress Centre, Vienna, Austria, 2012 Meeting Abstract, 2012

Vinardell T., Rolfe R.A., Buckley C.T., Meyer E.G., Murphy P. and Kelly D.J. , Hydrostatic Pressure Plays a Role in Both the Induction and Maintenance of a Chondrogenic Phenotype in Joint Tissue Derived Stem Cells, Annual Meeting of the Orthopaedic Research Society, San Francisco, California, USA, 2012 Meeting Abstract, 2012

Sheehy E.J., Vinardell T., Buckley C.T. and Kelly D.J. , Modulating Endochondral Ossification to Engineer Osteochondral Constructs, 18th Congress of the European Society of Biomechanics, Lisbon, Portugal, 2012 Meeting Abstract, 2012

A.J. Steward, S.D. Thorpe, C.T. Buckley, D.R. Wagner, D.J. Kelly, Cell-matrix interactions regulate mesenchymal stem cell response to hydrostatic pressure, Acta Biomaterialia, 8, (6), 2012, p2153-2159 Journal Article, 2012 TARA - Full Text DOI

Liu Y, Buckley C.T., Downey R., Mulhall K and Kelly D.J. , The role of environmental factors in regulating the development of cartilaginous tissues engineered using osteoarthrithic human infrapatellar fat pad derived stem cells., Annual Meeting of the Orthopaedic Research Society, San Francisco, California, USA, 2012 Meeting Abstract, 2012

Almeida H.V., Buckley C.T., Cunniffe G.M., Ahearne M., O'Brien F.J. and Kelly D.J., Combining freshly isolated CD271+ positive infrapatellar fat pad stem cells and a TGF-β3 releasing scaffold to develop a single stage therapy for cartilage repair, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Hofburg Congress Centre, Vienna, Austria, 2012 Meeting Abstract, 2012

Vinardell T, Rolfe RA, Buckley CT, Meyer EG, Ahearne M, Murphy P, Kelly DJ, Hydrostatic pressure acts to stabilise a chondrogenic phenotype in porcine joint tissue derived stem cells., European Cells & Materials, 23, 2012, p121-134 Journal Article, 2012 URL TARA - Full Text

Sheehy E.J., Vinardell T., Buckley C.T. and Kelly D.J., Channelled architectures accelerate in vivo mineralisation of engineered hypertrophic cartilaginous constructs, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Hofburg Congress Centre, Vienna, Austria, 2012 Meeting Abstract, 2012

Vinardell T., Sheehy E.J., Buckley C.T. and Kelly D.J. , A comparison of the functionality and in vivo phenotypic stability of cartilaginous tissues engineered from different stem cell sources, Annual Meeting of the Orthopaedic Research Society, San Francisco, California, USA, 2012 Meeting Abstract, 2012

Buckley, C.T., Ahearne, M., Downey R., Mulhall K.J. and Kelly D.J., Engineering Cartilage Grafts using Fibrin Hydrogels Seeded with Diseased Human Infrapatellar Fat Pad Derived Stem Cells, Annual Meeting of the Orthopaedic Research Society, San Francisco, California, USA, 2012 Meeting Abstract, 2012

Thorpe SD, Buckley CT, Steward AJ, Kelly DJ, European Society of Biomechanics S.M. Perren Award 2012: The external mechanical environment can override the influence of local substrate in determining stem cell fate., Journal of biomechanics, 45, (15), 2012, p2483-2492 Journal Article, 2012 DOI TARA - Full Text

Buckley CT, Meyer EG, Kelly DJ, The influence of construct scale on the composition and functional properties of cartilaginous tissues engineered using bone marrow-derived mesenchymal stem cells., Tissue engineering. Part A, 18, (3-4), 2012, p382-396 Journal Article, 2012 TARA - Full Text DOI

C.T. Buckley, D.J. Kelly, Expansion in the presence of FGF-2 enhances the functional development of cartilaginous tissues engineered using infrapatellar fat pad derived MSCs, Journal of the Mechanical Behavior of Biomedical Materials, 11, 2012, p102-111 Journal Article, 2012 TARA - Full Text DOI

Ahearne M, Buckley CT, Kelly DJ, A growth factor delivery system for chondrogenic induction of infrapatellar fat pad-derived stem cells in fibrin hydrogels., Biotechnology and Applied Biochemistry, 58, (5), 2011, p345-352 Journal Article, 2011 DOI

Mesallati T., Buckley C.T., Nagel T. and Kelly D.J., Introducing microchannels into chondrocyte-seeded agarose hydrogels influences matrix accumulation in response to dynamic compression and TGF-â3 stimulation., IFMBE Proceedings , 8th International Conference on Cell & Stem Cell Engineering (ICCE) , 30, 2011, pp26-30 Conference Paper, 2011 DOI

Vinardell T., Buckley C.T., Meyer E.G. and Kelly D.J. , Hydrostatic pressure and TGF-beta3 interact to regulate chondrogenesis of joint derived mesenchymal stem cells., Tissue Engineering and Regenerative Medicine International Society (TERMIS), Granada, Spain, 7th-10th June, 2011 Meeting Abstract, 2011

T. Vinardell, C.T. Buckley, S. D. Thorpe, and D. J. Kelly, Composition-function relations of cartilaginous tissues engineered from chondrocytes and mesenchymal stem cells isolated from bone marrow and infrapatellar fat pad, Journal of Tissue Engineering and Regenerative Medicine, 5, (9), 2011, p673-683 Journal Article, 2011 TARA - Full Text

Cox G., McGonagle D., Boxhall S.A., Buckley C.T., Jones E. and Giannoudis P.V., The use of the reamer-irrigator-aspirator to harvest mesenchymal stem cells., Journal of Bone and Joint Surgery, 93-B, (4), 2011, p517-524 Journal Article, 2011 DOI

Sheehy, E.J., Vinardell, T., Meyer, E.G., Buckley, C.T. and Kelly, D.J. , The oxygen environment regulates mesenchymal stem cell expansion and chondrogenic differentiation in an in vitro cartilage defect model., Annual Meeting of the Orthopaedic Research Society, Long Beach, California, USA, January 13th-16th, 2011 Meeting Abstract, 2011

Mesallati, T., Buckley, C.T. and Kelly, D.J. , Is self assembly using progenitor cells a better approach to engineering functional cartilage tissue than hydrogel encapsulation? , Tissue Engineering and Regenerative Medicine International Society (TERMIS), Granada, Spain, 2011 Meeting Abstract, 2011

Ahearne M.A., Buckley C.T. and Kelly D.J., Engineering a MSC seeded fibrin hydrogel containing TGF-beta 1 loaded gelatin microspheres for cartilage repair, Tissue Engineering and Regenerative Medicine International Society (TERMIS), Granada, Spain, 2011 Meeting Abstract, 2011

Meyer, E.G., Buckley, C.T., and Kelly, D.J. , The Effect of Cyclic Hydrostatic Pressure on the Functional Development of Cartilaginous Tissues Engineered Using Bone Marrow Derived Mesenchymal Stem Cells., ASME 2011 Summer Bioengineering Conference, Pennsylvania, US, June 22nd-25th, ASME, 2011 Meeting Abstract, 2011

Thorpe S.D., Buckley C.T., Steward, A.J and Kelly D.J. , Cell-Matrix Interactions Modulate Mesenchymal Stem Cell Response To Dynamic Compression, ASME 2011 Summer Bioengineering Conference, Pennsylvania, USA, June 22nd-25th, ASME, 2011 Meeting Abstract, 2011

62. Mesallati, T., Buckley, C.T., Nagel, T., and Kelly, D.J. , Scaffold Architecture Determines Chondrocyte Response to Externally Applied Dynamic Compression, Annual Meeting of the Orthopaedic Research Society, Long Beach, California, USA, 2011 Meeting Abstract, 2011

Meyer, E.G., Buckley, C.T., and Kelly, D.J. , The Functional Properties of Cartilaginous Tissues Engineered Using Bone-Marrow Derived MSCs Improve As They Are Scaled Towards Geometries of Clinical Relevance. , Annual Meeting of the Orthopaedic Research Society, Long Beach, California, USA., January 13th-16th, 2011 Meeting Abstract, 2011

Steward A.J., Thorpe S.D., Vinardell T., Buckley C.T., Wagner D.R. and Kelly D.J., Cell-Matrix interactions regulate mesenchymal stem cell response to extrinsic mechanical signals, European Society for Biomaterials, Dublin, Ireland, 2011 Meeting Abstract, 2011

E.G. Meyer, C.T. Buckley, A.J. Steward, D.J. Kelly, The effect of cyclic hydrostatic pressure on the functional development of cartilaginous tissues engineered using bone marrow derived mesenchymal stem cells, Journal of the Mechanical Behavior of Biomedical Materials, 4, (7), 2011, p1257-1265 Journal Article, 2011 URL TARA - Full Text DOI

Sheehy E.J., Buckley C.T. and Kelly D.J., Chondrocytes and bone marrow-derived mesenchymal stem cells undergoing chondrogenesis in agarose hydrogels of solid and channelled architectures respond differentially to dynamic culture conditions., Tissue Engineering & Regenerative Medicine., 5, (9), 2011, p747-758 Journal Article, 2011 DOI

Buckley, C.T., Meyer, E.G. and Kelly, D.J. , Expansion in the Presence of FGF-2 Modulates the Temporal Development of Cartilaginous Tissues Engineered Using Infrapatellar Fat Pad Derived MSCs, Annual Meeting of the Orthopaedic Research Society, Long Beach, California, USA, January 13th-16th, 2011 Meeting Abstract, 2011

Thorpe S.D., Buckley C.T., and Kelly D.J., Can dynamic compression in the absence of growth factors induce chondrogenic differentiation of bone marrow derived MSCs encapsulated in agarose hydrogels?, IFMBE Proceedings, IFMBE, 30, 2011, pp43-46 Conference Paper, 2011

Cox G, McGonagle D, Boxhall S., Buckley C.T., Jones E. and Giannoudis P.V. , The Reamer- Irrigator-Aspirator (RIA): A harvester of mesenchymal stem cells (MSCs)., 4th UK Mesenchymal Stem Cell Meeting, University of Leeds, UK, 14th April, 2010 Meeting Abstract, 2010

Meyer E.G., Buckley C.T., Haugh M.G. and Kelly D.J., The influence of expansion on the resulting functional properties of cartilaginous tissues generated from MSCs., 4th UK Mesenchymal Stem Cell Meeting, University of Leeds, UK, 14th April , 2010 Meeting Abstract, 2010

McAlinden A.B., Buckley C.T., and Kirby B.M. , Biomechanical evaluation of different numbers, sizes and placement configurations of ligaclips required to secure cellophane bands, Veterinary Surgery, 39, (1), 2010, p59-64 Journal Article, 2010 DOI

Meyer EG, Buckley CT, Thorpe SD, Kelly DJ., Low oxygen tension is a more potent promoter of chondrogenic, Journal of Biomechanics, 43, (13), 2010, p2516-2523 Journal Article, 2010 TARA - Full Text

Thorpe, S. D., Buckley, C. T., Vinardel, T., O'Brien, F. J., Campbell, V. A., Kelly, D. J. , The response of bone marrow derived mesenchymal stem cells to dynamic compression following TGF-â3 induced chondrogenic differentiation, Annals of Biomedical Engineering , 38, (9), 2010, p2896-2909 Journal Article, 2010 DOI

Sheehy, E.J., Buckley C.T., and Kelly D.J. , Cartilaginous tissue engineering using chondrocytes and bone marrow derived MSCs by a combination of rotational culture and modified scaffold architecture, Tissue Engineering and Regenerative Medicine International Society - EU Meeting, Galway, Ireland,, 2010 Oral Presentation, 2010

Meyer EG, Haugh MG, Buckley CT, Kelly DJ., Monolayer expansion and cell seeding density influence the functional properties of cartilaginous tissues engineered using MSCs., Orthopedic Research Society Meeting, New Orleans, LA, 2010 Meeting Abstract, 2010

Meyer E.G., Haugh M.G., Buckley C.T. and Kelly D.J. , Monolayer expansion and cell seeding density influence the functional properties of cartilaginous tissues engineered using MSCs., 56th Annual Meeting of the Orthopaedic Research Society, Morial Convention Center Hall H, New Orleans, Louisiana, March 6 - 9, 2010 Meeting Abstract, 2010

Mesallati, T.S., Buckley, C.T., Kelly, D.J., The effect of introducing microchannels into dynamically compressed chondrocyte-seeded hydrogels, 8th International Conference on Cell & Stem Cell Engineering (ICCE), , Dublin, 11-12th June 2010. , 2010 Oral Presentation, 2010

Buckley C.T., Vinardell T. and Kelly D.J., Infrapatellar Fat Pad Derived MSCs as a Potential Alternative to Chondrocytes for Cartilage Repair- Chondrogenesis in a Physiologically Relevant Low Oxygen Tension Environment., 4th UK Mesenchymal Stem Cell Meeting, University of Leeds, UK, 14th April, 2010 Meeting Abstract, 2010

Buckley C.T., O'Kelly K.U., Maintaining cell depth viability: on the efficacy of a trimodal scaffold pore architecture and dynamic rotational culturing., Journal of Materials Science: Materials in Medicine, 21, (5), 2010, p1731-1738 Journal Article, 2010 DOI

Vinardell T, Buckley C, Thorpe S, Kelly D, Functional properties of cartilaginous tissues generated from mesenchymal stem cells isolated from different tissue sources., 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, USA , 2010 Meeting Abstract, 2010

Buckley C.T., O'Kelly K.U, Fabrication and characterization of a porous multidomain hydroxyapatite scaffold for bone tissue engineering investigations, Journal of Biomedical Materials Research, 93B, (2), 2010, p459 - 467 Journal Article, 2010 DOI

Meyer E.G., Buckley C.T., Thorpe S.D. and Kelly D.J., Low oxygen tension: A more potent chondrogenic stimulus than dynamic compression for bone marrow derived MSCs in agarose hydrogel culture., 56th Annual Meeting of the Orthopaedic Research Society, Morial Convention Center Hall H, New Orleans, Louisiana, USA, March 6 - 9, 2010 Meeting Abstract, 2010

Buckley, C T; Vinardell, T; Thorpe, S D; Jones, E; McGonagle, D; Kelly, D J. , Functional Properties of Cartilaginous Tissues Engineered From Infrapatellar Fat Pad Derived Mesenchymal Stem Cells. , Journal of Biomechanics , 43, (5), 2010, p920-926 Journal Article, 2010 TARA - Full Text

Thorpe S.D., Buckley C.T., Vinardell T., O'Brien F.J., Campbell V.A. and Kelly D.J., Dynamic Compression Post TGF-â3 Induced Differentiation Augments Chondrogenesis of Mesenchymal Stem Cells., 56th Annual Meeting of the Orthopaedic Research Society, Morial Convention Center Hall H, New Orleans, Louisiana, USA, March 6 - 9, 2010 Meeting Abstract, 2010

Meyer EG, Buckley CT, Thorpe SD, Kelly DJ., Low oxygen tension: A more potent chondrogenic stimulus than dynamic compression for bone marrow derived MSCs in agarose hydrogel culture., . 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, LA, March 6 - 9, 2010 Meeting Abstract, 2010

Cox G., Giannoudis P.V., Boxall S., Buckley C.T., Jones E., and McGonagle D, Extraction of high numbers of mesenchymal stem cells (MSCs) from intramedullary cavities of long bones, Injury Extra, 41, (12), 2010, pp155 - 156 Conference Paper, 2010

Meyer E.G., Buckley C.T., Kelly D.J, The Effect of Cyclic Hydrostatic Pressure and Low Oxygen Tension on the Chondrogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cells., 8th International Conference on Cell & Stem Cell Engineering (ICCE), , Dublin, 11-12th June, 2010 Oral Presentation, 2010

Buckley CT, Vinardell T, Kelly DJ., Oxygen tension differentially regulates the functional properties of cartilaginous tissues engineered from infrapatellar fat pad derived MSCs and articular chondrocytes., Osteoarthritis Cartilage., 18, (10), 2010, p1345-1354 Journal Article, 2010 TARA - Full Text

Thorpe S.D., Buckley C.T., Vinardell T., O'Brien F.J., Campbell V.A. and Kelly D.J., Dynamic compression of mesenchymal stem cells following cytokine induced differentiation augments glycosaminoglycan synthesis., 4th UK Mesenchymal Stem Cell Meeting, University of Leeds, UK, 14th April, 2010 Meeting Abstract, 2010

Vinardell T, Buckley C, Thorpe S, Kelly D , Functional properties of cartilaginous tissues generated from mesenchymal stem cells isolated from different tissue sources., European Cells and Materials , X European Cells and Materials Conference, Stem Cells for musculoskeletal regeneration, Davos, Switzerland, June 29- July 1, 18, (1), 2009, pp63 Meeting Abstract, 2009

Vinardell T, Thorpe S, Buckley C, Kelly D. , Chondrogenesis of bone marrow mesenchymal stem cells and chondrocytes- an in vitro cartilage defect repair model., 3rd UK Mesenchymal Stem Cell Meeting, Sheffield, UK, 2009 Meeting Abstract, 2009

Vinardell T, Thorpe S, Buckley C, Kelly D., Chondrogenesis of bone marrow mesenchymal stem cells and chondrocytes- an in vitro cartilage defect repair model., 55th Annual Meeting of the Orthopaedic Research Society, Las Vegas, USA, 2009 Meeting Abstract, 2009

Buckley, C.T., Thorpe, S.D., Kelly, D.J. , Engineering of large cartilaginous constructs through the use of microchanneled hydrogels and rotational culture, Tissue Engineering A, 15, (11), 2009, p3213-3220 Journal Article, 2009 DOI TARA - Full Text

Thorpe S.D., Buckley C.T., Vinardell T., O'Brien F.J., Campbell V.A. and Kelly D.J., Dynamic compression can inhibit chondrogenesis of mesenchymal stem cells, 3rd UK Mesenchymal Stem Cell Meeting, University of Sheffield, United Kingdom, 2009 Meeting Abstract, 2009

Vinardell, T; Thorpe, S D; Buckley, C T; Kelly, D J, Chondrogenesis and integration of mesenchymal stem cells within an in vitro cartilage defect repair model, Annals of Biomedical Engineering, 37, (12), 2009, p2556-2565 Journal Article, 2009 TARA - Full Text

Vinardell T, Buckley C.T. and Kelly D.J. , Mechanical Behaviour of Cartilaginous Tissues Engineered from Mesenchymal Stem Cells Isolated from Different Tissue Sources, Third International Conference on Mechanics of Biomaterials and Tissues, Clearwater Beach, Florida, USA, 13 - 17 December , 2009 Meeting Abstract, 2009

Thorpe, S., Vinardell, T., Buckley, C., Kelly, D.J., Dynamic Compressive Loading can Inhibit Chondrogenesis of Bone Marrow Derived Mesenchymal Progenitor Cells, Trans. 55th Annual Meeting of the Orthopaedic Research Society, Las Vegas, 2009 Meeting Abstract, 2009

Haut Donadue, T., Thorpe, S., Buckley, C., Kelly, D.J., The Response of Inner vs. Outer Zone Meniscal Cells to Unconfined Compression. , Trans. 55th Annual Meeting of the Orthopaedic Research Society, Las Vegas, 2009 Meeting Abstract, 2009

Vinardell, T., Thorpe, S., Buckley, C., Kelly, D.J., Chondrocytes demonstrate superior integration than mesenchymal stem cells in a cartilage explant defect model., Trans. 55th Annual Meeting of the Orthopaedic Research Society, Las Vegas, 2009 Meeting Abstract, 2009

Thorpe, S., Buckley, C., Kelly, D.J., Chondrogenesis of Mesenchymal Stem Cells is Differentially Regulated by Temporal Application of Dynamic Compression, ECM X: Stem Cells for Musculoskeletal Regeneration, Davos, Switzerland, 2009 Meeting Abstract, 2009

Vinardell T, Buckley C, Thorpe S, Kelly D, Functional properties of cartilaginous tissues generated from mesenchymal stem cells isolated from different tissue sources., 3rd International Conference on Mechanics of Biomaterials and Tissues, Clearwater Beach, USA, 2009 Oral Presentation, 2009

Buckley, C.T., Thorpe, S.D., O'Brien, F.J., Robinson, A.J., Kelly, D.J. , The effect of concentration, cooling rate and cell seeding density on the initial mechanical properties of agarose hydrogels., Mechanical Behaviour of Biomedical Materials , 2, (5), 2009, p512 - 521 Journal Article, 2009 TARA - Full Text DOI

Buckley, C., Thorpe, S., Kelly, D.J. , The Effect of Dynamic Rotational Culturing on Chondrocytes in a Microchannel Agarose Hydrogel Environment., Trans. 55th Annual Meeting of the Orthopaedic Research Society, Las Vegas, 2009 Meeting Abstract, 2009

Buckley CT, Thorpe SD, O'Brien FJ, Robinson AJ, Kelly DJ, The effect of concentration, thermal history and cell seeding density on the initial mechanical properties of agarose hydrogels., Journal of the Mechanical Behavior of Biomedical Materials, 2, (5), 2009, p512-21 Journal Article, 2009 DOI URL

Buckley, C.T., O'Brien F.J., Robinson, A., Kelly, D.J., Cell/Hydrogel mixing and delivery - Thermally regulated injection device, 2008, EP 08394020.5 Patent, 2008

Thorpe S.D., Buckley C.T., O'Brien F.J., Robinson A.J. and Kelly D.J., Fabrication Methodologies Regulate the Initial Mechanical Properties Of Cell Seeded Hydrogels, 16th Conference of the European Society of Biomechanics, Lucerne, Switzerland, 2008 Meeting Abstract, 2008

Kelly, K., Buckley, C., O'Donnell, G.E., Ervine, P., Surface Finish in Micro-drilling of PTFE, 25th International Manufacturing Conference, Dublin Institute of Technology, 3 - 5 Sept, edited by Robert Simpson , 2008, pp65 - 72 Conference Paper, 2008 TARA - Full Text

Thorpe, S. D., Buckley, C. T., Vinardell, T., O'Brien, F. J., Campbell, V. A., Kelly, D. J. , Dynamic compression can inhibit chondrogenesis of mesenchymal stem cells. , Biochemical and Biophysical Research Communications, 337, (2), 2008, p458-462 Journal Article, 2008 URL DOI TARA - Full Text

S. D. Thorpe, C.T. Buckley, T Vinardell, F.J. O'Brien, V.A.Campbell and D.J. Kelly , Dynamic compression can inhibit chondrogenesis of mesenchymal stem cells, Biochemical and Biophysical Research Communications, 377, (2), 2008, p458 - 462 Journal Article, 2008

Buckley, C.T. and O'Kelly, K.U. , Development of a Hydroxyapatite Bone Tissue Engineering Scaffold with a Trimodal Pore Structure, Key Engineering Materials , 361-363, 2008, p931 - 934 Journal Article, 2008

Regular Scaffold Fabrication Techniques for Investigations in Tissue Engineering in, editor(s)P.J. Prendergast & P.E McHugh , Topics in Biomechanical Engineering, 2004, pp147 - 166, [C.T. Buckley and K.U. O'Kelly] Book Chapter, 2004

Non-Peer-Reviewed Publications

Irwin, P.P. and Buckley, C.T. , Glucose mapping of bovine intervertebral disc organ culture models. An experimental and numerical analysis. , Proceedings of the 23rd Annual Conference of the Section of Bioengineering of the Royal Academy of Medicine in Ireland, 2017 Poster, 2017

Buckley C.T. , Cellular microencapsulation and microenvironmental challenges for intervertebral disc regeneration, AOSpine Masters Symposium-Novel and Emerging Technologies in Translational Medicine , Bern, Switzerland, 22 - 23 September , 2017 Invited Talk, 2017

Buckley, C. T., Engineering Injectable Cellular Microcapsules for the Intervertebral Disc Microenvironment Niche., ORS Musculoskeletal Summer Symposium, Dublin, Ireland, July 6th-7th, 2017 Invited Talk, 2017

Buckley C.T. , Engineering solutions to regenerate-Injectable cellular microcapsules for intervertebral disc repair. Cell Matrix and Regenerative Medicince (CMRM) Seminar Series, Cell Matrix and Regenerative Medicince (CMRM) Seminar Series, Manchester University, UK, 9th June , 2017, Wellcome Trust Centre for Cell-Matrix Research and the Division of Cell Matrix Biology and Regenerative Medicine Invited Talk, 2017

Mandal, P. and Buckley, C.T., Comparison of bone marrow stem cells and nasal chondrocytes for nucleus pulposus regeneration, Proceedings of the 23rd Annual Conference of the Section of Bioengineering of the Royal Academy of Medicine in Ireland, 2017 Poster, 2017

Buckley C.T. , Cells and Injectable Biomaterials - Engineering for the Disc Microenvironment, Orthopaedic Research Society Spine Section Meeting, San Diego Convention Center, USA, March 19th, 2017 Invited Talk, 2017

Buckley C.T. , SCIENCE & SPORTS INJURIES- How tissue engineering will reduce the need for complex surgery?, A World of Science in Sport, Public Lecture, Global Room, Trinity College Dublin, Jul , 2014 Invited Talk, 2014

Buckley C.T. , Regenerating the Intervertebral Disc- Biomaterial Strategies, Stem Cells and Microenvironmental Effects, Icahn School of Medicine at Mount Sinai, New York, USA, Nov , 2014 Invited Talk, 2014

Buckley C.T. , Regenerative Medicine & Tissue Engineering, Trinity Centre for Bioengineering Winter Symposium, Trinity College Dublin, Dec , 2010 Invited Talk, 2010

Vinardell T, Buckley C, Thorpe S, Kelly D, Functional properties of cartilaginous tissues generated from mesenchymal stem cells isolated from different tissue sources., 16th Annual Conference of the Section of Bioengineering, Dublin, Ireland, 2010 Oral Presentation, 2010

Buckley C.T. , Articular Cartilage Repair- Biophysical and Biochemical Environments for Mesenchymal Stem Cell Based Therapies, Seminar Series, University of Sheffield, UK, May , 2010 Invited Talk, 2010

Buckley C.T. , Mesenchymal Stem Cell Based Therapies for Articular Cartilage Repair, Mesenchymal Stem Cell Group Seminar Series, University of Leeds, July , 2010 Invited Talk, 2010

Sheehy E.J., Buckley C.T. and Kelly D.J., Rotational culture differentially regulates chondrogenesis of bone marrow derived MSCs and chondrocytes., Proceedings of the 16th Annual Conference of the Section of Bioengineering of the Royal Academy of Medicine in Ireland. , 2010 Oral Presentation, 2010

Vinardell T, Thorpe S, Buckley C, Kelly D. , Chondrogenesis of bone marrow mesenchymal stem cells and chondrocytes- an in vitro cartilage defect repair model., 15th Annual Conference of the Section of Bioengineering, Limerick, Ireland, edited by McGloughlin and M. Walsh , 2009 Oral Presentation, 2009

Buckley C.T. , Regenerative Medicine & Tissue Engineering, Trinity Centre for Bioengineering Winter Symposium, Trinity College Dublin, Dec, 2009 Invited Talk, 2009

Buckley C.T. , Mechanobiology of mesenchymal stem cells, Stem Cell Workshop, Trinity College Dublin, Mar, 2009 Invited Talk, 2009

Buckley C.T. & O'Kelly K.U., Fabrication of a hydroxyapatite scaffold with a tri-modal pore distribution for bone tissue engineering, Proceedings of the 12 Annual Conference of the Section of Bioengineering of the Royal Academy of Medicine in Ireland, Galway, edited by McHugh P.E., O'Mahoney, D. and Fitzpatrick D. , 2006, pp89 Oral Presentation, 2006

Buckley C.T., Prina-Mello A., O'Kelly K.U., Development of biodegradable elastomeric scaffolds for mechanobiology investigations, Proceedings of the 11th Annual Conference of the Section of Bio-engineering of the Royal Academy of Medicine in Ireland, Galway, edited by FitzPatrick D.P. and Senouci M. , 2005, pp13- Oral Presentation, 2005

Buckley C.T. and O'Kelly K.U., A New technique for fabricating regular 3D scaffolds for bone tissue engineering applications, Proceedings of the tenth Annual Conference of the Section of Bioengineering of the Royal Academy of Medicine in Ireland, Limerick, edited by D.P. Fitzpatrick, B.A.O. McCormack and T.M. McGlouhlin , 2004, pp19- Oral Presentation, 2004

Daniel John Kelly, Grainne Cunniffe, Henrique Almeida, Rajalakshmanan Eswaramoorthy, Conor Buckley, Peyno Pedro Diaz, 'A method for making a porous scaffold suitable for use in repair of osseous, chondral, or osteochondral defects in a mammal', 0, 17 August 2015 Patent, URL

Research Expertise

Description

The overall goal of my laboratory is to develop novel biomaterial and cell based strategies to regenerate or repair damaged tissues and restore biomechanical function using minimal invasive strategies (MIS). *Intervertebral Disc, Cartilage, Nerve Tissue Engineering *Cellular Microencapsulation for Therapeutic Regeneration *Cryopreservation strategies *Biomimetics & Decellularisation of Native Tissues as Scaffold Matrices *Scaffold Fabrication Technology (Injectable Biomaterials, Bio Printing) *Strategies for Innovative Intraoperative Clinical Intervention *Bioreactor Systems and Biophysical Stimulation *3D Printing of Medical Devices

Projects

  • Title
    • A Bioactive Collagen-Based Conduit For Peripheral Nerve Repair
  • Summary
    • Peripheral nerve injury remains a major clinical problem. It is estimated that 5% of multiple trauma patients have root, plexus or peripheral nerve injuries. These injuries are typically caused by transection or burns or as a result of degenerative conditions such as amyotrophic lateral sclerosis, Parkinson's disease and multiple sclerosis. Ideally, prompt surgical intervention is required but if the injury size is >5mm or direct suturing is not possible, autografts are utilised and are currently the accepted clinical 'gold-standard' for treatment. However, autografts are hampered by limited availability of donor tissue and the relatively poor prognosis for functional recovery at both the donor and recipient sites. As a result, new approaches are currently being investigated to develop artificial nerve grafts which mimic the properties of autologous grafts. Commercially available nerve guidance conduits have been developed and present promising alternatives to autografts. It is estimated that the worldwide market for peripheral nerve repair conduits is over $100 million. However, challenges arising with their use have created a major gap in the market for a drug and cell free conduit that can facilitate repair of large defects. This is the focus of the current project which will be centred on two approaches which will be investigated in parallel: (i) the development of a bioactive collagen-based conduit containing inductive macromolecules and (ii) the development of a decellularized nerve graft.
  • Funding Agency
    • Science Foundation Ireland
  • Date From
    • 1 March 2017
  • Date To
    • 28-Feb 2020
  • Title
    • Intraoperative Single Stage Procedure for Intervertebral Disc Regeneration (INSPIRE)
  • Summary
    • 97% of individuals over the age of 50 exhibit degeneration of the intervertebral disc (IVD). Recent investigations have shown that cell-based strategies can retard degenerative changes. However, the challenge for clinical translation remains in identifying suitable cell sources and biomaterial delivery systems to support such cells within the harsh and complex biochemical (low pH) and biomechanical environment of the disc. Intraoperative single stage cell therapies are highly attractive, avoiding the need for two hospital visits and costly in vitro cell expansion. In addition, developing injectable biomaterial systems is pivotal for minimally invasive therapeutic approaches. The overall aim of this proposal is to develop an autologous single stage therapy for intervertebral disc (IVD) regeneration. This consists of tissue harvesting from nasal septum, rapid cell isolation, development of biomimetic injectable cellular microcapsules (Aim 1) combined with pH buffering biomaterials to regulate the acidic microenvironment that exists in native disc tissue (Aim 2) and then evaluating the proposed strategy in both ex-vivo organ culture and in-vivo models (Aim 3). If successful, the strategies outlined will create a paradigm shift in which the scientific community and clinical investigators approach early stage disc degeneration.
  • Funding Agency
    • Science Foundation Ireland
  • Date From
    • Dec 2016
  • Date To
    • Dec 2020
  • Title
    • Towards a Single Stage Procedure for Intervertebral Disc Repair using Nasal Derived Chondrocytes (NasDisc)
  • Summary
    • Autologous Disc Cell Transplantation (ADCT) has been in clinical use for the treatment of degenerated IVD in a similar manner to those currently used for cartilage repair of the knee joint. Although these therapies have made it to the clinical setting there are significant concerns regarding its effectiveness due to issues of cell leakage following cell injection into the disc, the limited tissue forming capacity of culture expanded NP cells derived from degenerated tissue and questions as to whether the number of nucleus pulposus (NP) cells that can be isolated from a degenerated disc is sufficient to meet the requirements for successful treatment. Therefore, there is a need to identify an alternative cell source for the development and clinical translation of next generation therapies for IVD regeneration. The objective of this proposal is to determine the feasibility and the subsequent tissue forming capacity of freshly isolated nasal septal derived chondrocytes on porous microcarriers to augment tissue regeneration as part of a single stage procedure.
  • Funding Agency
    • Research Capacity Building Grant by TCD Research Committee
  • Date From
    • May 2015
  • Date To
    • May 2016
  • Title
    • Over-the-wire Drug Delivery Device
  • Summary
    • A recently developed and patented technology (Delivering drugs to desired locations within a mammal, PCT/US11/26040) at the Mayo Clinic may offer significant clinical and commercial opportunity to sustainably deliver pharmacologic agents over long periods of time. This is an Over-the-Wire Drug Delivery system that allows the placement of an anchored device on the posterior wall of the vertebral body, near the dorsal root ganglion and disc. This device could potentially provide a durable therapy and prolong the residence for short acting agents (e.g. Clonidine, dexmedetomidine, infliximab, etanercept). The objective of this project is to assess the commercial feasibility for further development and identify key tasks for continued investment.
  • Funding Agency
    • Enterprise Ireland
  • Date From
    • Apr 2015
  • Date To
    • Jan 2016
  • Title
    • Microencapsulation of Mesenchymal Stem Cells for Minimal Invasive Regeneration of the Intervertebral Disc
  • Summary
    • The primary objective of this proposal is to develop a biomimetic hydrogel that can be combined with cells to generate microbeads formed through electrohydrodynamic (EHD) spraying for minimal invasive delivery to the IVD. The second objective is to investigate if the combination of microencapsulated mesenchymal stem cells (MSCs) in a biomimetic hydrogel will augment or improve regeneration of the nucleus pulposus in an ex vivo full organ culture model of the IVD. The third and final objective of this proposal is to investigate the combined effects of biophysical stimuli and challenging biochemical nutrient environments that normally exist in vivo on the regenerative potential of microencapsulated MSCs. If successful, the approaches outlined will create a paradigm shift in the manner in which the scientific community and clinical investigators approach biological treatment of disc degeneration.
  • Funding Agency
    • Irish Research Council
  • Date From
    • Oct 2014
  • Date To
    • Oct 2018
  • Title
    • Injectable Biomimetic Hydrogels for Intervertebral Disc Regeneration (BIODISC)
  • Summary
    • : Support funding to facilitate the preparatory work leading to a proposal for the coordination of a research project (ERC-STG 2014) under the Horizon 2020 Programme.
  • Funding Agency
    • Enterprise Ireland
  • Date From
    • April 2014
  • Date To
    • Jun 2014
  • Title
    • Nucleus Pulposus Regeneration of the Intervertebral Disc (NuGenDisc)
  • Summary
    • Personal award to develop international collaborations with world-class renowned researchers, to, learn new techniques, and build on and establish new collaborations and networks. International laboratories: Prof. James Iatridis, School of Medicine at Mount Sinai in New York City and Prof. Benjamin Gattenbein, University of Bern, Switzerland.
  • Funding Agency
    • Irish Research Council
  • Date From
    • Mar 2014
  • Date To
    • Nov 2014
  • Title
    • Natural ECM Derived Injectable Microparticles for Regeneration of the Intervertebral Disc (DiscReGen)
  • Summary
    • Recent evidence suggests that degeneration initiates within the central region of the disc called the nucleus pulposus (NP). The objective of this grant is to develop an injectable microparticle encapsulated Extracellular Matrix (ECM) derived from NP tissue fabricated using electrospraying technology. It is hypothesised that these microparticles will provide key biochemical cues to stimulate repair and regeneration of the disc by the patient's own cells. This technology and approach for regeneration or repairing the intervertebral disc is highly attractive, and could be performed at an earlier stage of tissue degeneration to alleviate back pain thus reducing the number of patients requiring spinal fusion or artificial implant replacement surgery.
  • Funding Agency
    • Enterprise Ireland
  • Date From
    • Sep 2012
  • Date To
    • May 2014
  • Title
    • Injectable Hydrogel Composites for Stem Cell Repair of the Nucleus Pulposus
  • Summary
    • Autologous cell implantation therapies have been proposed for the treatment of degenerated IVD similar to those currently used for cartilage repair in the knee. Recent investigations have demonstrated that reimplantation of extracted nucleus pulposus (NP) cells can retard degenerative changes, and injection of autologous. NP cells have been clinically tested in humans with herniated discs. However, it is believed that the number of NP cells that can be isolated from a degenerated disc is insufficient to meet the requirements for successful treatment. As an alternative, mesenchymal stem cells (MSCs) possess significant potential and perhaps provide a more readily available and clinically feasible source of cells for IVD repair therapies. A key challenge with using MSCs is developing suitable biomaterials that can be injected into the NP compartment whilst providing the optimal biochemical and biophysical environment to facilitate differentiation. The proposed project will utilise state of the art tissue engineering and biomaterial strategies using a combination of MSCs, injectable growth factor releasing hydrogel based systems.
  • Funding Agency
    • Higher Education Authority (HEA).
  • Date From
    • Apr 2012
  • Date To
    • Apr 2016
  • Title
    • Isolation and characterization of different mesenchymal stem cell populations
  • Summary
    • Awarded €15,000 to acquire expertise and training in the isolation, purification and characterisation of mesenchymal stem cells (MSCs) from different tissue sources (bone marrow, intramedullary adipose tissue, infrapatellar fat pad and synovium membrane) using magnetic bead separation technology and FACS. International Laboratory Collaborators: Prof. Dennis McGonagle and Dr. Elena Jones, Leeds Institute of Molecular Medicine, University of Leeds, United Kingdom.
  • Funding Agency
    • Science Foundation Ireland (SFI)
  • Date From
    • Apr 2009
  • Date To
    • Jan 2010

Keywords

Biomaterials; Tissue Engineering

Recognition

Representations

Quality and Qualifications Ireland (QQI) panel expert. QQI is the national agency responsible for the National Framework of Qualifications (NFQ). Dec 2016-Jan 2017

Visceral- Living Art Experiment, TCD Science Gallery, Ireland. Exhibiting demonstrating ten years of SymbioticA's challenging work at the frontier between fine art and biotechnology revolving around the nature of the living and non-living. Contribution: Chief Scientific Advisor. 28th Jan- 25th Feb 2011

Transformations, TCD Science Gallery, Ireland, HEA celebration of 10 years of PRTLI funding. Contribution: Co-organised the Exploring Bioengineering exhibit demonstrating the research undertaken by TCBE since its inception. 27th Nov- 2nd Dec 2008

Techno Threads- Victimless leather, TCD Science Gallery, Ireland. Contribution: Setup, installation and management of exhibition piece which demonstrated how tissue cultures could be used to create semi-living clothes 26th Apr- 25th Jul 2008

Awards and Honours

Science Foundation Ireland, Career Development Award- Principal Investigator 2016

Irish Research Council (IRC) New foundations Award- Principal Investigator 2014

Global Medical Discovery Series. Contribution- Principal Investigator 2014

Engineers Ireland Biomedical Research Medal- Co-Investigator 2014

Best overall MSc Thesis in Bioengineering. Principal Investigator 2012-2016

Engineers Ireland Biomedical Research Medal. Co-Investigator 2012

European Society of Biomechanics S.M. Perren Award. Co-Investigator 2012

Science Foundation Ireland Travel Fellowship- Investigator 2009

Memberships

Educating Students in Engineering and Medicine (ESEM), Co- Founding member and Treasurer 2015 – Present

Tissue Engineering and Regenerative Medicine International Society (TERMIS) 2009 – Present

European Society of Biomechanics (ESB) 2009 – Present

Orthopaedic Research Society (ORS) 2009 – Present