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Cellular and Molecular Methodologies

Researchers use a range of cellular and molecular approaches to investigate functioning of the nervous system. These include:

Electrophysiology

  • In vivo and in vitro analysis of synaptic plasticity (LTP, LTD, PPF)
  • Single cell recording
  • Patch clamp
  • Neuronal ensemble recording with multielectrode arrays in the freely-moving animal
  • Transcranial direct current stimulation

Genetic Manipulation

  • Transgenic and knockout mice
  • Drosophila-based models
  • siRNA and viral gene delivery

Cell culture systems

  • Primary cultures of neurons and glia
  • Primary T cell cultures
  • Endothelial cell cultures
  • Stem cell cultures

Analyses include:

Techniques include:

  • Cell death and viability assays
  • Cytokine, neurotrophin and neurotransmitter release
  • Cell signalling
  • Reactive oxygen and nitrogen species production
  • Lysosomal stability
  • Mitochondrial function
  • ELISA
  • HPLC
  • Real time PCR
  • Flow cytometry and Immunohistochemistry
  • Immunoprecipitation and Western Immunoblotting
  • Proteomics