Research Activities – Professor C.J. Dorman
PROKARYOTIC GENE REGULATION
Charles J. Dorman, B.Sc. (N.U.I.), M.A., Ph.D., Sc.D., F.T.C.D., M.R.I.A., Professor of Microbiology and Final-year Project Co-Ordinator.
E-mail: email@example.com *This chair was formerly the Professorship of Bacteriology and Preventive Medicine, established in 1919.
- Phase-variable expression of type 1 fimbriae in Escherichia coli K-12. Molecular biology of the site-specific recombination system: interplay of recombinases, accessory factors, DNA topology. Structure-function analysis of the FimB and FimE site-specific recombinases. Analysis of protein-DNA interactions in vivo and in vitro. Influence of transcriptionally- induced variations in DNA supercoiling on recombination.
- Stationary-phase control of spv virulence gene transcription in Salmonella typhimurium. Role of the SpvR regulatory protein, RpoS sigma factor, H-NS nucleoid-associated protein. Isolation of novel regulators. Influence of DNA topology. Microbial Evolution.
- The virulence gene regulatory cascade of Shigella flexneri. Structure and functions of the VirF and VirB regulatory proteins. Influence of DNA topology. Genetic instability within the S. flexneri virulence plasmid.
- Nucleoid-associated protein H-NS and its homologue StpA in enteric bacteria. Regulation of hns and stpA gene expression.