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Grass organelle genomes: phylogenomics and sequence evaluation

Kerstin Diekman

Kerstin Diekmann

diekmank@tcd.ie

Supervisors: Dr. Trevor Hodkinson and Dr. Susanne Barth

We are working on the phylogenomics of perennial ryegrass (Lolium perenne). Lolium is the most important forage grass species of Ireland and most temperate regions of the world. We are sequencing its entire chloroplast and mitochondrial genomes (outsourced to a private company) and investigating them in a phylogenomic context. More specifically we are investigating their sequence structure and variation in comparison to other sequenced grasses, comparing them phylogenetically to other grasses, developing molecular markers for both genomes applicable in plant breeding, and to gaining first insights into the horizontal gene transfer between nuclear and organellar sequences of the species.

Publications:

Diekmann, K., Hodkinson, T.R., Fricke, E. and Barth, S. (2008) An optimized chloroplast DNA extraction protocol for grasses (Poaceae) proves suitable for whole plastid genome sequencing and SNP detection. PLoS ONE,  3, (7) (In Press)

Diekmann, K., Hodkinson, T.R., and Barth, S. (2008) Features of the complete chloroplast genome of perennial ryegrass (Lolium perenne L.). Proceedings of the Agricultural Research Forum 2008, 138. ISBN 1 84170 504 7

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Last updated 14 December 2009 botany@tcd.ie.